FastQCFastQC Report
Thu 26 May 2016
SRR1926672_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926672_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678819
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10560.15556429622624No Hit
GTCCTAAAGTGTGTATTTCTCATTT9110.13420366843002332No Hit
CTGTAGGACGTGGAATATGGCAAGA7630.11240109661043665No Hit
GTCCTACAGTGTGCATTTCTCATTT7210.10621388028325665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGC250.003981943720.4113677
CGTGCAA250.00726828118.3936259
TAGGACC2950.016.3681854
GTGTAGG500.002397561716.0400831
AAAGGCG400.002807134515.6665665
AGGACGG507.314815E-414.6221285
ATAAGAC450.00444663714.620123
GTATAGA658.230085E-414.3949471
CTAGGAC857.838007E-714.1901173
TGTAGGA6850.014.0169772
GATATAC1801.0913936E-1113.3667361
GTCCTAC7400.013.1861051
CCTAGAC500.00893564213.1581083
CCAACTT552.9663972E-413.15572113
GTCCTAA4500.013.0696981
TGTAGAA2750.012.9684342
CTGTAGG6600.012.9616831
TCCCACA853.474439E-412.9097532
CCAGTAC600.002005897512.7926043
CTCTAGG1152.528599E-612.7855731
Produced by FastQC (version 0.11.5)