FastQCFastQC Report
Thu 26 May 2016
SRR1926672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926672_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678819
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA22070.3251234865258633No Hit
GTCCTACAGTGGACATTTCTAAATT20760.3058252641720399No Hit
CTTTAGGACGTGAAATATGGCGAGG19040.2804871401654933No Hit
GTCCTAAAGTGTGTATTTCTCATTT18380.2707643716513533No Hit
CTGTAGGACCTGGAATATGGCGAGA15040.22156127038282666No Hit
GTCCTACAGTGTGCATTTCTCATTT14960.2203827529871733No Hit
GTATCAACGCAGAGTACTTTTTTTT10550.15541698155178332No Hit
CTGAAGGACCTGGAATATGGCGAGA10420.15350189078384666No Hit
GTACATGGGAAGCAGTGGTATCAAC7450.10974943247021665No Hit
CCCATGTACTCTGCGTTGATACCAC7380.10871822974901998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAG250.00601889919.005327
ACCGAGG250.00602403819.002518
TATGTCG602.6294452E-514.21199116
TGTCGAG602.6294452E-514.21199118
TAGGACC5000.013.9242894
GCTATAC551.8925632E-413.8732983
CTAGGAC1102.575689E-913.0062163
CTAGACA603.9542024E-412.7162454
CCAACGA604.17986E-412.63288119
GGCGAGG5450.012.51698319
CTACACT1700.012.3422384
TCCAACG1001.4886427E-712.317059518
CTGGACT1103.597961E-812.1382344
GTCCTAT802.7569677E-511.9179461
TAAAGTA657.754723E-411.7380735
CCTACAC1552.3646862E-1111.6916083
TTTAGTA907.1085524E-611.6591522
GTCCTAC12500.011.5937781
AGGACCT8750.011.4446215
ATGTCGA752.1225316E-411.36959417
Produced by FastQC (version 0.11.5)