##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926672_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 678819 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.919118351136312 31.0 28.0 31.0 16.0 34.0 2 28.42093989708597 31.0 28.0 31.0 20.0 34.0 3 28.753211091616468 31.0 28.0 33.0 23.0 34.0 4 32.520343419969095 35.0 32.0 37.0 26.0 37.0 5 32.138801359419816 35.0 32.0 37.0 22.0 37.0 6 32.249707212084516 35.0 33.0 37.0 23.0 37.0 7 32.047956819122625 35.0 32.0 37.0 19.0 37.0 8 32.03910173404103 35.0 32.0 37.0 19.0 37.0 9 33.20782712328323 37.0 33.0 39.0 17.0 39.0 10 33.076009952579405 37.0 33.0 39.0 17.0 39.0 11 33.3451229267301 37.0 33.0 39.0 19.0 39.0 12 33.139110720236175 37.0 33.0 39.0 18.0 39.0 13 33.28410813486364 37.0 33.0 39.0 19.0 39.0 14 33.84823200293451 38.0 33.0 40.0 17.0 41.0 15 33.98779645236801 38.0 33.0 40.0 17.0 41.0 16 33.95965493010655 38.0 33.0 40.0 17.0 41.0 17 33.89312173053494 38.0 33.0 40.0 17.0 41.0 18 33.863798744584344 38.0 33.0 40.0 17.0 41.0 19 33.898018470313886 39.0 33.0 40.0 18.0 41.0 20 33.883303207482406 39.0 33.0 40.0 16.0 41.0 21 33.78977312066987 39.0 33.0 40.0 11.0 41.0 22 33.72914576639723 39.0 33.0 40.0 10.0 41.0 23 33.36709196413182 38.0 33.0 40.0 2.0 41.0 24 33.44755081987982 38.0 33.0 40.0 2.0 41.0 25 33.2280490086459 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5852975474758288 1101 2 0.8575193075173253 1101 3 0.8042134448112712 1101 4 0.5351782738066291 1101 5 0.5460076473436857 1101 6 0.20894381340522017 1101 7 0.4950889535288212 1101 8 0.5159699939962827 1101 9 0.9281509864811639 1101 10 0.8246662003858205 1101 11 0.9561031048148791 1101 12 0.5478114482873977 1101 13 0.3985815028805604 1101 14 0.9020043870491747 1101 15 0.9402110096761973 1101 16 1.0237426548468491 1101 17 0.9316353965779385 1101 18 1.0664843580890846 1101 19 1.0927038456881704 1101 20 0.9787503249234675 1101 21 1.0179324191130306 1101 22 0.9078071014036908 1101 23 1.0500109264836084 1101 24 1.0021114007629492 1101 25 1.0072625534361563 1102 1 0.7196133706276626 1102 2 0.7976411966883887 1102 3 0.7671682610924897 1102 4 0.5084919662308778 1102 5 0.8339417451320159 1102 6 0.41726113110284757 1102 7 0.6449382769469025 1102 8 1.0008649351291048 1102 9 1.134082067034818 1102 10 1.034539534296826 1102 11 0.7782395646965412 1102 12 1.0260261103889619 1102 13 0.9018205494818048 1102 14 1.297697606714081 1102 15 1.0330372639133998 1102 16 0.959886000896411 1102 17 0.9880919010738012 1102 18 1.0637270766219729 1102 19 1.115861899460711 1102 20 1.2093028995341086 1102 21 1.1398082592395866 1102 22 1.2404190830988426 1102 23 1.4606140549575173 1102 24 1.2878873684961647 1102 25 1.267664569962072 1103 1 0.0338334600415493 1103 2 -0.11251425222675238 1103 3 -0.029681745059843223 1103 4 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0.7448632396952632 2202 22 0.48370712684424433 2202 23 0.6764576217413207 2202 24 0.7083719648183973 2202 25 0.8746206695371512 2203 1 -0.15547804373536422 2203 2 -0.07416278305767321 2203 3 -0.44038355198806656 2203 4 -0.6045971775734884 2203 5 -0.38724734968586816 2203 6 -0.39625821159595986 2203 7 -0.34105065814251745 2203 8 -0.19555231833071218 2203 9 0.0073509982238846305 2203 10 -0.1730215653221947 2203 11 -0.02726549530610356 2203 12 -0.06298768244229791 2203 13 -0.24156824770251717 2203 14 0.0574362835216462 2203 15 0.07523529470291379 2203 16 0.20124545726245202 2203 17 0.29430389881716934 2203 18 0.23177627670276024 2203 19 0.13597654177807073 2203 20 0.22294482454066866 2203 21 0.013841008217482909 2203 22 0.15314651622995967 2203 23 0.2537448024542144 2203 24 0.1374640184238487 2203 25 0.3901632988671153 2204 1 0.20846870134345608 2204 2 0.3275851545393209 2204 3 0.12279540741731054 2204 4 -0.03792234297412733 2204 5 0.10372708468479175 2204 6 -0.1387396295490788 2204 7 0.36772986950593634 2204 8 0.16952160146018613 2204 9 0.7007431406726354 2204 10 0.35336902521934377 2204 11 0.22969766731743846 2204 12 0.26800962213991397 2204 13 0.20294698549195545 2204 14 0.570921540403134 2204 15 0.5290292994403174 2204 16 0.7254307059760023 2204 17 0.7854743635725399 2204 18 0.5913286739910077 2204 19 0.7996700809433079 2204 20 0.5776127980765651 2204 21 0.8006089572495867 2204 22 0.5802313810506732 2204 23 0.8974922499752509 2204 24 0.7667378892071497 2204 25 0.6845846008355849 2205 1 0.4682045286560772 2205 2 0.449527148836264 2205 3 0.5213999314484532 2205 4 0.12811565770551425 2205 5 0.09650961500998534 2205 6 0.1920036885662526 2205 7 0.1764986888549558 2205 8 0.160736529876484 2205 9 0.5080256192861725 2205 10 0.6536054213601759 2205 11 0.4541816588662755 2205 12 0.5935175044737377 2205 13 0.6821374764571928 2205 14 1.0254692111624095 2205 15 0.9248775922169372 2205 16 0.8297316382632829 2205 17 0.8907164479955085 2205 18 0.8155504135711098 2205 19 0.8286345180988732 2205 20 0.8460524357994856 2205 21 0.8475055191632137 2205 22 0.8325244236167535 2205 23 1.0822668610297228 2205 24 0.9444863637133025 2205 25 0.9095916188460222 2206 1 0.022998661855190505 2206 2 0.32844449955774024 2206 3 0.3288266035064993 2206 4 0.2589741877252223 2206 5 0.5481512051652544 2206 6 0.29606814759507216 2206 7 0.26273483692183675 2206 8 0.2614657798998117 2206 9 0.5197900424038764 2206 10 0.5731461550213268 2206 11 0.3289204550240683 2206 12 0.39904682575375716 2206 13 0.3979752656900786 2206 14 0.6652221031378289 2206 15 0.5566416760170654 2206 16 0.685736569678717 2206 17 0.7435558291933617 2206 18 0.7354375339242267 2206 19 0.634126373063232 2206 20 0.6259965049524041 2206 21 0.790133986211238 2206 22 0.5767588524745833 2206 23 0.8655659327133876 2206 24 0.7450361995684247 2206 25 0.5017212890576133 2207 1 -1.2301259533721058 2207 2 -0.17081082395741376 2207 3 0.104072736301692 2207 4 0.22683171069680697 2207 5 -0.003178604084872916 2207 6 0.2820003357752725 2207 7 0.34089004266611056 2207 8 0.4509134254212803 2207 9 0.7180722431402771 2207 10 0.29126471475230886 2207 11 0.1265430553467013 2207 12 0.11296584140343668 2207 13 -0.17542980144906295 2207 14 -5.319320207773124 2207 15 -2.358527811794378 2207 16 -1.160198526252728 2207 17 -0.8942621887080904 2207 18 -0.5743075369852519 2207 19 -0.4206555212462746 2207 20 -0.12989916938501977 2207 21 0.12878892170159162 2207 22 -0.015177911587841209 2207 23 0.23309803797398132 2207 24 -0.036513770971460247 2207 25 -0.15190988704429031 2208 1 0.695685099403228 2208 2 1.150095977050146 2208 3 0.9972960072191128 2208 4 1.081599901849323 2208 5 1.175765889253718 2208 6 1.1028292433450986 2208 7 1.360970363951246 2208 8 1.322810080049873 2208 9 1.5518253917548819 2208 10 1.3939990778975897 2208 11 1.0327957740299993 2208 12 1.2711077288524777 2208 13 0.9691181637113289 2208 14 1.5097208936603153 2208 15 1.3308756458649782 2208 16 1.4355021606257665 2208 17 1.4068273078170535 2208 18 1.4947970931220809 2208 19 1.2386102572328852 2208 20 1.2321373899505588 2208 21 1.303545378258299 2208 22 1.2494432936360766 2208 23 1.7093526787031479 2208 24 1.3947043003060955 2208 25 1.5097554139582101 2209 1 -0.08792404004079657 2209 2 0.30097317003260216 2209 3 0.6493500638531202 2209 4 0.8398226059083456 2209 5 0.9257307742606429 2209 6 0.7756475113236334 2209 7 0.9243648587385493 2209 8 0.9741850195361685 2209 9 1.0905916622698797 2209 10 1.000835932957898 2209 11 0.921507837905196 2209 12 0.6623664820315796 2209 13 0.7530348150364929 2209 14 0.9881684700439521 2209 15 0.8644176236232113 2209 16 1.0008494581349936 2209 17 1.1518433652556297 2209 18 1.0923833635007085 2209 19 1.1188933590800048 2209 20 1.1260028347518372 2209 21 0.9447287876172936 2209 22 0.8859744777954646 2209 23 1.3027046945764909 2209 24 1.348550926198456 2209 25 1.463586357426479 2210 1 -0.34684639620166635 2210 2 -0.2541767768275278 2210 3 0.3563526023441277 2210 4 0.5318476002813313 2210 5 0.5841971560240466 2210 6 0.5893449285618217 2210 7 0.9259061386567424 2210 8 0.8755453406709357 2210 9 1.1766424827703972 2210 10 0.9756366025352108 2210 11 0.6927186966246808 2210 12 0.5912589218598043 2210 13 0.7191490158291955 2210 14 0.9097286507642366 2210 15 0.8535168692307806 2210 16 0.8089481835233272 2210 17 0.9760182066185763 2210 18 0.9405717633570134 2210 19 1.158233261982346 2210 20 1.099082607167098 2210 21 1.165407576833104 2210 22 1.2431452304438864 2210 23 0.9969141625666111 2210 24 1.0402420262758199 2210 25 0.9913897652351338 2211 1 -1.0892080788702465 2211 2 -0.30504448052298017 2211 3 0.3958235666313925 2211 4 0.6204112724402862 2211 5 0.9376226996403574 2211 6 0.6130606119123314 2211 7 0.7773221307054925 2211 8 0.9336747376376486 2211 9 0.9094853975469519 2211 10 1.1782523376786784 2211 11 0.8153581695722067 2211 12 0.8998908020662739 2211 13 0.8873418839615752 2211 14 1.1134717790437278 2211 15 0.7722072625140228 2211 16 1.0812357465768727 2211 17 1.0456892717457436 2211 18 0.9394581003133098 2211 19 0.8479766541847198 2211 20 0.818045924174335 2211 21 0.9788247756044157 2211 22 1.0650399984396515 2211 23 1.410794065449089 2211 24 1.332880637278656 2211 25 1.3799475639657075 2212 1 0.9342604534509995 2212 2 0.17465738055891578 2212 3 0.47560926023116323 2212 4 0.4853517513526029 2212 5 0.7634217759584345 2212 6 0.8131970302823177 2212 7 0.8128198672275104 2212 8 0.7379654383250802 2212 9 1.106211356667167 2212 10 0.6668800755725002 2212 11 0.685712704911424 2212 12 0.8792878176286365 2212 13 0.6709465298072956 2212 14 0.9705114242035435 2212 15 0.9001844600691626 2212 16 0.38726711518083334 2212 17 0.9551257648028866 2212 18 0.7969108111520953 2212 19 0.5731378412531782 2212 20 0.7412263774796202 2212 21 0.8812879274022407 2212 22 0.9625166396517173 2212 23 1.2782281807111815 2212 24 1.037232307069992 2212 25 0.8061726618973353 2213 1 0.28652618887961623 2213 2 0.019606245612546758 2213 3 -0.5356071694686797 2213 4 -1.2156002732586906 2213 5 -1.9073190571670366 2213 6 -0.5728136124382246 2213 7 -0.29765656080940417 2213 8 -0.08307887862844154 2213 9 -0.5954099952909289 2213 10 -0.5139131396931518 2213 11 -0.5775241787669714 2213 12 -0.8065108021435421 2213 13 -0.7387940538794098 2213 14 -7.378952282456474 2213 15 -3.4525385797578956 2213 16 -2.0212138027538167 2213 17 -1.042313330565868 2213 18 -5.008269200488382 2213 19 -2.9017312033963627 2213 20 -5.253227767361153 2213 21 -2.1167747648734725 2213 22 -1.9198937702593142 2213 23 -4.462451124186135 2213 24 -2.0600321811648143 2213 25 -1.5949705990987582 2214 1 0.5758675576312022 2214 2 0.30297663548635967 2214 3 -1.5161602870939426 2214 4 -2.0567535118052938 2214 5 -0.35693776486894535 2214 6 -0.1091768055418072 2214 7 -0.5181634552762802 2214 8 -0.5015515640574222 2214 9 -5.365870081496144 2214 10 -1.419355575283035 2214 11 -0.7817661774797386 2214 12 -0.9992155395296933 2214 13 -1.2944422507861653 2214 14 -2.503670945300467 2214 15 -2.2170345132901623 2214 16 -1.582366846265998 2214 17 -1.9421885085347768 2214 18 -1.1940248613625286 2214 19 -4.4914928713307525 2214 20 -2.089529524621309 2214 21 -3.967618288755439 2214 22 -6.167275433122807 2214 23 -6.120948774021333 2214 24 -5.799274082360377 2214 25 -3.4729804678405927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27540.0 3 14.0 4 475.0 5 3177.0 6 3842.0 7 3079.0 8 2238.0 9 1846.0 10 1674.0 11 1516.0 12 1446.0 13 1429.0 14 1475.0 15 1431.0 16 1498.0 17 1583.0 18 1747.0 19 2029.0 20 2736.0 21 3428.0 22 4420.0 23 5476.0 24 6603.0 25 7761.0 26 8910.0 27 9711.0 28 11176.0 29 13414.0 30 16211.0 31 20589.0 32 26202.0 33 33485.0 34 43744.0 35 57229.0 36 76778.0 37 110861.0 38 162046.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.820174753101526 20.466401460306038 8.875664613234171 30.837759173358258 2 12.554299455973972 22.052799405874065 41.30147765249527 24.09142348565669 3 18.289964030420155 25.93906243322935 29.305408404297488 26.46556513205301 4 11.556632272629479 18.697742136084855 38.92936401976994 30.816261571515724 5 12.084576564629609 39.92853722326593 35.75054333812208 12.236342873982393 6 30.354037524251172 39.6726979132163 18.17802725595041 11.795237306582122 7 27.2255281583582 32.16558581992055 22.468080206554166 18.140805815167088 8 23.411630962750802 40.71161767528383 20.016535514446428 15.860215847518935 9 26.189653594134338 15.761452122994605 19.986770963445927 38.062123319425126 10 14.412463240277459 30.66158660561164 34.63104730585857 20.29490284825233 11 33.51418957938033 22.755919199538013 25.824091642997725 17.905799578083936 12 23.740866234531527 25.381555686505596 32.360636417206834 18.51694166175604 13 29.353627402886485 23.23343925258427 25.672528317563298 21.740405026965952 14 19.71113161983895 24.42289717559435 27.74457395083606 28.121397253730642 15 21.507921893299354 34.19414715349543 22.967818919735127 21.33011203347009 16 20.25636266138679 29.174695493839163 30.20371123328678 20.365230611487263 17 20.03638827914377 30.776970786252004 28.7517494364973 20.434891498106925 18 20.030347672362993 28.64113140836771 31.87271655863288 19.455804360636417 19 23.12483518879446 27.465980757417057 27.625525370245153 21.783658683543333 20 23.112653025146948 29.011947378500615 28.57544821083956 19.299951385512884 21 21.645124319036917 27.531532752519823 27.269459560589144 23.553883367854123 22 22.36929137222146 30.46438004828975 27.06273690041086 20.10359167907793 23 20.821192131027757 30.03441275864812 28.36047364683906 20.783921463485058 24 22.907453293538435 27.92812169460265 29.339406671046852 19.825018340812065 25 21.494684149972233 29.129709097712354 28.82520966561042 20.550397086704997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 169.0 1 169.0 2 159.5 3 150.0 4 150.0 5 150.0 6 263.0 7 376.0 8 376.0 9 376.0 10 649.5 11 923.0 12 923.0 13 923.0 14 1710.0 15 2497.0 16 2497.0 17 2497.0 18 4868.5 19 7240.0 20 7240.0 21 7240.0 22 12278.5 23 17317.0 24 17317.0 25 17317.0 26 26807.5 27 36298.0 28 36298.0 29 36298.0 30 49183.0 31 62068.0 32 62068.0 33 62068.0 34 70316.5 35 78565.0 36 78565.0 37 78565.0 38 83559.5 39 88554.0 40 88554.0 41 88554.0 42 90295.5 43 92037.0 44 92037.0 45 92037.0 46 92373.0 47 92709.0 48 92709.0 49 92709.0 50 85322.5 51 77936.0 52 77936.0 53 77936.0 54 65140.5 55 52345.0 56 52345.0 57 52345.0 58 42959.0 59 33573.0 60 33573.0 61 33573.0 62 26536.5 63 19500.0 64 19500.0 65 19500.0 66 14634.0 67 9768.0 68 9768.0 69 9768.0 70 7028.0 71 4288.0 72 4288.0 73 4288.0 74 2977.5 75 1667.0 76 1667.0 77 1667.0 78 1130.0 79 593.0 80 593.0 81 593.0 82 380.0 83 167.0 84 167.0 85 167.0 86 113.5 87 60.0 88 60.0 89 60.0 90 37.0 91 14.0 92 14.0 93 14.0 94 9.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08912537804628332 2 0.025780068029916663 3 0.027989788146766663 4 0.03137802565927 5 0.02121331312176 6 0.37756751063243665 7 0.2088922083795533 8 0.21935155026597664 9 0.22406561984858997 10 0.012816376677729998 11 0.0016204614190233333 12 0.0027989788146766665 13 0.0 14 0.07616168669409666 15 5.892586978266666E-4 16 0.00220972011685 17 0.004272125559243333 18 0.0027989788146766665 19 0.0017677760934799998 20 0.00132583207011 21 1.4731467445666666E-4 22 0.0 23 1.4731467445666666E-4 24 7.365733722833333E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 678819.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.03616226855205 #Duplication Level Percentage of deduplicated Percentage of total 1 92.42376227380326 74.89666997089999 2 5.701013616396335 9.239765290270363 3 0.8911670562457711 2.1665027453496046 4 0.32183878394708026 1.0432231968099626 5 0.15713232841233635 0.636670043142875 6 0.10051929724635118 0.4887418849665679 7 0.06621362265101215 0.3755988508675266 8 0.04392577825983498 0.2847661195869139 9 0.039106165638200276 0.2852112225922264 >10 0.20935210475544136 3.2099218164977112 >50 0.023531979588148472 1.290062895378161 >100 0.0193359452373911 3.321555242439668 >500 0.0016417311981556148 0.8215934147976778 >1k 0.0014593166205827685 1.9397173064007067 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2207 0.3251234865258633 No Hit GTCCTACAGTGGACATTTCTAAATT 2076 0.3058252641720399 No Hit CTTTAGGACGTGAAATATGGCGAGG 1904 0.2804871401654933 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1838 0.2707643716513533 No Hit CTGTAGGACCTGGAATATGGCGAGA 1504 0.22156127038282666 No Hit GTCCTACAGTGTGCATTTCTCATTT 1496 0.2203827529871733 No Hit GTATCAACGCAGAGTACTTTTTTTT 1055 0.15541698155178332 No Hit CTGAAGGACCTGGAATATGGCGAGA 1042 0.15350189078384666 No Hit GTACATGGGAAGCAGTGGTATCAAC 745 0.10974943247021665 No Hit CCCATGTACTCTGCGTTGATACCAC 738 0.10871822974901998 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.4731467445666666E-4 0.0 8 0.0 0.0 0.0 1.4731467445666666E-4 0.0 9 0.0 0.0 0.0 1.4731467445666666E-4 0.0 10 0.0 0.0 0.0 1.4731467445666666E-4 0.0 11 0.0 0.0 0.0 1.4731467445666666E-4 0.0 12 0.0 0.0 0.0 1.4731467445666666E-4 0.0 13 0.0 0.0 0.0 2.946293489133333E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGAG 25 0.006018899 19.00532 7 ACCGAGG 25 0.006024038 19.00251 8 TATGTCG 60 2.6294452E-5 14.211991 16 TGTCGAG 60 2.6294452E-5 14.211991 18 TAGGACC 500 0.0 13.924289 4 GCTATAC 55 1.8925632E-4 13.873298 3 CTAGGAC 110 2.575689E-9 13.006216 3 CTAGACA 60 3.9542024E-4 12.716245 4 CCAACGA 60 4.17986E-4 12.632881 19 GGCGAGG 545 0.0 12.516983 19 CTACACT 170 0.0 12.342238 4 TCCAACG 100 1.4886427E-7 12.3170595 18 CTGGACT 110 3.597961E-8 12.138234 4 GTCCTAT 80 2.7569677E-5 11.917946 1 TAAAGTA 65 7.754723E-4 11.738073 5 CCTACAC 155 2.3646862E-11 11.691608 3 TTTAGTA 90 7.1085524E-6 11.659152 2 GTCCTAC 1250 0.0 11.593778 1 AGGACCT 875 0.0 11.444621 5 ATGTCGA 75 2.1225316E-4 11.369594 17 >>END_MODULE