FastQCFastQC Report
Thu 26 May 2016
SRR1926671_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926671_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641551
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7350.1145661062020011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGCG300.002011143616.46989416
GTATAAT500.00246608415.9722331
TCCTAGG603.8376756E-415.9163412
ATTTAGA1551.2732926E-1114.5982791
CCTAGGG450.00457596214.5569573
GTCCTAT658.497202E-414.3340551
CTATACC754.3240216E-614.2639394
TGTAGGA5850.014.22559452
CTGTAGG5750.014.120381
ATAGGGG550.001075570413.8952783
CAGTACT851.0350504E-613.8444114
TAGGACC2100.013.7545124
GACCATC450.00730175413.56647
GTCCTAC5500.013.5521971
GAAATGT1700.013.460096
ACAACAC652.478051E-413.4371913
GCCTTAG600.00827791513.3101941
GTATTAA600.00827791513.3101941
GTTTAGG700.001487137913.3101941
GAATAAT600.00827791513.3101941
Produced by FastQC (version 0.11.5)