FastQCFastQC Report
Thu 26 May 2016
SRR1926671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926671_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641551
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14890.23209378521738722No Hit
CTGTAGGACGTGGAATATGGCAAGA14280.22258557776388782No Hit
CTTTAGGACGTGAAATATGGCGAGG13840.21572719861710138No Hit
GTCCTAAAGTGTGTATTTCTCATTT13710.21370085932373267No Hit
GTCCTACAGTGTGCATTTCTCATTT12260.19109938259000453No Hit
CTGTAGGACCTGGAATATGGCGAGA9570.1491697464426055No Hit
CCCATGTACTCTGCGTTGATACCAC9170.14293485630916325No Hit
GTACATGGGAAGCAGTGGTATCAAC7820.12189210210879571No Hit
GAGTAAGCAGTGGTATCAACGCAGA7630.11893052929541066No Hit
CTGAAGGACCTGGAATATGGCGAGA7460.1162807009886977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGACG350.002166716416.2875218
CGGTAGG400.00515698214.2985131
TAGGACC4600.013.889514
AGGACCT7050.012.5795135
GTAATAC550.002977480212.1377833
TGGCGAA550.003125811712.05926618
GGCGAGG4250.012.03919
TCTAGGA1353.45608E-1012.01028252
CTAGGAC1353.45608E-1012.0093413
GGACCTG6850.011.9723066
TGGCGAG9650.011.88074418
TAGGACA2500.011.8247414
AACTGAC657.9993985E-411.694527
TCCAACG658.190215E-411.66170918
GTCCTAC8350.011.530141
GACCTGG7200.011.48133857
TGTAGGA10750.011.4450932
GTAGGAC10500.011.4441963
GGACTGG1104.7132744E-711.2699146
TACTGCA1104.938029E-711.2293977
Produced by FastQC (version 0.11.5)