FastQCFastQC Report
Thu 26 May 2016
SRR1926670_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926670_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670175
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7120.10624090722572463No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAC408.837626E-519.288143
TAGCACT556.674058E-515.6779974
TGTAGGA5900.015.6706582
TAGGACC3150.015.3980334
GCCTTAT707.9517034E-515.3581451
CTGTAGG6200.015.172361
AGGACCT3950.014.5924865
GGACCTG4000.014.404086
GTCCTAG751.4311212E-414.3342681
GTAGGAC6400.013.7772423
CTAATAC500.00863591613.2261523
TCCTATA853.3015956E-412.9877962
TAGGACG5050.012.5928464
GGTTTGC550.002467059812.4453248
GTCCTAC6700.012.4355121
TCCTACA6900.012.3995812
TTAGGAC3500.012.2814273
ATGGGTT600.002817208212.2267345
AGGACGT4900.012.1910885
CTACACT1151.709268E-712.1845294
Produced by FastQC (version 0.11.5)