##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926670_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 670175 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.144596560599844 27.0 7.0 30.0 2.0 31.0 2 16.394613347260044 16.0 2.0 30.0 2.0 31.0 3 20.19430298056478 25.0 15.0 31.0 2.0 31.0 4 22.45018838363114 28.0 7.0 35.0 2.0 37.0 5 22.78754952064759 29.0 2.0 35.0 2.0 37.0 6 23.668613421867423 31.0 2.0 35.0 2.0 37.0 7 23.589963815421346 31.0 7.0 35.0 2.0 37.0 8 23.807311523109636 31.0 10.0 35.0 2.0 37.0 9 26.523684112358712 32.0 17.0 37.0 2.0 39.0 10 27.21843399112172 32.0 24.0 37.0 2.0 39.0 11 24.259576976162943 30.0 8.0 37.0 2.0 39.0 12 25.409485582124073 31.0 15.0 37.0 2.0 39.0 13 21.96778901033312 27.0 2.0 37.0 2.0 39.0 14 25.451314955049053 31.0 17.0 38.0 2.0 40.0 15 26.556396463610252 31.0 17.0 38.0 2.0 40.0 16 26.932501212369903 31.0 17.0 39.0 2.0 40.0 17 26.265120304398106 31.0 16.0 38.0 2.0 40.0 18 26.370031708135933 31.0 17.0 38.0 2.0 40.0 19 26.765544820382736 31.0 18.0 39.0 2.0 40.0 20 27.001147461483942 31.0 19.0 39.0 2.0 40.0 21 24.838415339277056 30.0 8.0 38.0 2.0 40.0 22 25.80447793486776 31.0 10.0 38.0 2.0 40.0 23 25.809391576826947 31.0 2.0 38.0 2.0 40.0 24 24.788544783079047 31.0 2.0 39.0 2.0 40.0 25 24.6019293468124 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.784247751651744 1101 2 10.685210159262997 1101 3 7.49410664382475 1101 4 8.872203758713589 1101 5 8.660447443263234 1101 6 8.024269446053339 1101 7 7.764620571771452 1101 8 6.498677898975959 1101 9 6.154531107748049 1101 10 5.5588201036825176 1101 11 8.45209841085374 1101 12 6.119054220872378 1101 13 10.706081145321637 1101 14 7.845849396690763 1101 15 6.917071192612369 1101 16 6.681432325691539 1101 17 2.561358764676612 1101 18 5.052438657335792 1101 19 5.63537857416388 1101 20 3.5414619765914424 1101 21 0.8340969493279644 1101 22 4.840177302568602 1101 23 5.962973468779612 1101 24 7.345839409840938 1101 25 7.597309851445477 1102 1 5.3840056313698454 1102 2 10.660241898244756 1102 3 6.954503862585931 1102 4 8.0722737220418 1102 5 7.721501845646703 1102 6 6.543476737961569 1102 7 6.534634603742333 1102 8 6.636651084940844 1102 9 5.599484511385356 1102 10 5.022264264692502 1102 11 7.733931135610931 1102 12 5.378269924102089 1102 13 9.72818955703617 1102 14 7.252896040219742 1102 15 5.201873035242045 1102 16 5.404228972168415 1102 17 6.067508069563434 1102 18 6.146296427392276 1102 19 4.8494922999469345 1102 20 5.259402067543771 1102 21 4.890095125564802 1102 22 5.4097353937489 1102 23 5.758809471593867 1102 24 7.2393725186103275 1102 25 6.9517597942563505 1103 1 5.134534606720042 1103 2 9.811168081936163 1103 3 6.992065779282559 1103 4 8.721776724641426 1103 5 7.601728191094594 1103 6 7.4717901283628265 1103 7 7.5828320294362115 1103 8 7.318398249738475 1103 9 6.205938213563513 1103 10 5.136656858144814 1103 11 6.165941875007682 1103 12 4.701815353487227 1103 13 8.795304293757816 1103 14 7.295810611207951 1103 15 6.469474908143489 1103 16 6.075082531825551 1103 17 3.6842555874171126 1103 18 2.307197458884062 1103 19 4.44696539261238 1103 20 4.891399171672578 1103 21 7.239860038936211 1103 22 2.500603903503393 1103 23 4.942293915619874 1103 24 6.422690579902483 1103 25 2.6301582021764958 1104 1 5.348208416077263 1104 2 10.223634680417483 1104 3 6.8718438965021384 1104 4 7.540121204261688 1104 5 7.346542553727975 1104 6 7.0831525015274615 1104 7 7.136973739138 1104 8 7.223366949203818 1104 9 6.088561405125297 1104 10 4.964282616484091 1104 11 8.21361868588296 1104 12 5.078693758987757 1104 13 9.434602482548428 1104 14 7.138109060213981 1104 15 6.251682462566642 1104 16 5.673122818832944 1104 17 6.135063473752503 1104 18 3.7706151801045102 1104 19 4.486146287926378 1104 20 3.879344358198292 1104 21 2.6529484815194024 1104 22 4.684437057463658 1104 23 5.092971222555363 1104 24 6.418626974134348 1104 25 5.604602846652263 1105 1 3.314826730721162 1105 2 9.80615579997976 1105 3 6.364352088787861 1105 4 5.701307056409718 1105 5 2.4256484672054093 1105 6 5.138529346670715 1105 7 6.122093090970367 1105 8 3.230261997756262 1105 9 3.8346997570478543 1105 10 3.575191885040745 1105 11 6.822769243559655 1105 12 3.956812088663373 1105 13 5.162205975919438 1105 14 3.691249185879137 1105 15 4.207151752768969 1105 16 4.662481866423356 1105 17 5.303423212801896 1105 18 2.915467568644331 1105 19 2.641179658337947 1105 20 1.6764905867405169 1105 21 2.848898936980426 1105 22 4.366250183158876 1105 23 2.102892155724348 1105 24 5.092955807973748 1105 25 5.717773637019938 1106 1 6.632005031814515 1106 2 11.60750146860886 1106 3 8.008675049258844 1106 4 9.251706551783982 1106 5 8.97241045206664 1106 6 7.866872492171137 1106 7 7.549279802492656 1106 8 7.632238448555253 1106 9 6.266659386988984 1106 10 5.502000127261102 1106 11 8.15622172463619 1106 12 5.574516041564969 1106 13 4.685481837988405 1106 14 5.979474585374511 1106 15 6.047921307016239 1106 16 5.971018451789213 1106 17 6.390513204391468 1106 18 6.5145634475341545 1106 19 6.250410104090683 1106 20 5.680338357976851 1106 21 6.030816514776895 1106 22 6.3776463143615665 1106 23 6.6991915669943225 1106 24 7.675293907216808 1106 25 6.718446471334488 1107 1 3.7713380408333883 1107 2 -14.028326958640932 1107 3 -5.903933077229469 1107 4 1.618474294881544 1107 5 4.349315385854013 1107 6 4.464865335505976 1107 7 4.412028835576983 1107 8 4.887780751027183 1107 9 3.1607042927226807 1107 10 2.7079286996014247 1107 11 5.355982885473942 1107 12 2.9082483856919232 1107 13 4.465850506942292 1107 14 3.7611509129245526 1107 15 3.2255721167859477 1107 16 2.949656955101613 1107 17 3.592813804184111 1107 18 -1.107052347620133 1107 19 1.6105275097073743 1107 20 1.9426402058601298 1107 21 -1.0570788498428563 1107 22 1.905946060346757 1107 23 -1.1022017560044404 1107 24 2.217669420813195 1107 25 3.090129280446117 1108 1 5.228230605706745 1108 2 10.769331904235322 1108 3 0.13143777671312762 1108 4 6.094263462821896 1108 5 7.401043462015696 1108 6 7.56209640929325 1108 7 7.803309213678244 1108 8 7.438115763181244 1108 9 5.90368257333202 1108 10 5.038895610101292 1108 11 7.158892528071245 1108 12 2.7373794972667618 1108 13 3.345843964617398 1108 14 4.889876795151423 1108 15 4.795731501356215 1108 16 5.031314757670042 1108 17 0.03418667722353064 1108 18 3.718480485262944 1108 19 4.982936664450275 1108 20 5.26266000203287 1108 21 5.29662122968023 1108 22 2.1468925542810062 1108 23 4.968190275895033 1108 24 5.695694823081578 1108 25 6.310779057386679 1109 1 3.352485336483216 1109 2 -14.028326958640932 1109 3 -5.829423101539055 1109 4 2.049265706954575 1109 5 1.233395892194217 1109 6 2.312316479078536 1109 7 3.575395822721376 1109 8 3.5801425769471855 1109 9 3.17328760598053 1109 10 2.302826606640984 1109 11 0.34068609682133655 1109 12 0.9835847412980954 1109 13 2.2741070059239163 1109 14 2.819969890461909 1109 15 3.081569576324725 1109 16 2.8470989734542584 1109 17 3.4879358266248524 1109 18 3.307626846148434 1109 19 1.7471992134954348 1109 20 0.6442805553925552 1109 21 -0.3277095120418174 1109 22 2.1785579902942693 1109 23 2.5383302808176467 1109 24 3.6890746316787784 1109 25 4.008414031108607 1110 1 2.912452002412401 1110 2 9.064518015805405 1110 3 6.160492604560638 1110 4 7.29338688957661 1110 5 7.906010330566744 1110 6 3.8822877911282134 1110 7 6.143063248991869 1110 8 6.806314984891316 1110 9 4.918010571827857 1110 10 4.474024347003972 1110 11 2.146497434830291 1110 12 4.991068682683228 1110 13 9.707275881341516 1110 14 7.033427415333403 1110 15 6.2842665386468965 1110 16 6.0786217947556125 1110 17 6.537568193122638 1110 18 6.378974294838304 1110 19 5.394785074539556 1110 20 5.680721627097682 1110 21 3.317682347660096 1110 22 5.623657808136848 1110 23 5.930654594777394 1110 24 7.092062898380842 1110 25 7.353096435586583 1111 1 4.843286472172132 1111 2 9.60093322423521 1111 3 7.497992126624304 1111 4 8.079134757272502 1111 5 8.305984982239664 1111 6 7.8394207962077935 1111 7 7.553135456321357 1111 8 7.798205644386812 1111 9 5.96803882317564 1111 10 5.434048335867704 1111 11 7.058456605614289 1111 12 6.534257842095883 1111 13 8.39502980714597 1111 14 6.413956784698183 1111 15 5.5905330982558255 1111 16 5.442530308536462 1111 17 6.688656967495618 1111 18 6.481952935686497 1111 19 5.485824511721731 1111 20 4.8099421589593945 1111 21 7.642458142415116 1111 22 7.253820051591241 1111 23 7.047857328998063 1111 24 7.238717839676102 1111 25 7.818911596718394 1112 1 -2.0771804702623875 1112 2 8.049771513345487 1112 3 5.996539899805949 1112 4 -0.15269300213486403 1112 5 4.310865960326424 1112 6 3.113735716321205 1112 7 5.690316555638713 1112 8 5.809036659661302 1112 9 4.477952494007049 1112 10 3.382742672465369 1112 11 -0.4331715678682464 1112 12 3.970631537173414 1112 13 8.176056160334873 1112 14 5.779347072419739 1112 15 3.8047499613047684 1112 16 4.1680523833720144 1112 17 5.199854920547342 1112 18 5.0030751443132 1112 19 4.840136101169165 1112 20 4.555547726252254 1112 21 -1.4178770129054108 1112 22 2.9773063274127267 1112 23 4.178824478187906 1112 24 5.959626377612526 1112 25 5.887652449735821 1113 1 -18.784311200313322 1113 2 -14.028326958640932 1113 3 -7.68724354196819 1113 4 -5.906777421759362 1113 5 -1.1750093671427209 1113 6 -0.5647720638485154 1113 7 -21.190837943512392 1113 8 -9.957369186651533 1113 9 -5.2935565229754005 1113 10 -4.382253116948057 1113 11 -5.421170266277262 1113 12 -3.3391169876148155 1113 13 -2.1426551139437926 1113 14 -2.4549460838329473 1113 15 -2.8213424185127387 1113 16 -3.2087371159222045 1113 17 -2.8684138278614455 1113 18 -3.8355260671908127 1113 19 -3.2940580799357733 1113 20 -3.936763245527022 1113 21 -1.3451438375506761 1113 22 -2.4067556608578897 1113 23 -2.525905690250621 1113 24 -2.3936335504082855 1113 25 -2.4925620114823417 1114 1 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2204 7 6.088417375636546 2204 8 6.055543240113806 2204 9 4.448566228067666 2204 10 3.900081833683444 2204 11 5.398515147203579 2204 12 5.269315145645521 2204 13 3.443032583892048 2204 14 4.999319618747808 2204 15 5.154400468836467 2204 16 5.173022340866986 2204 17 -0.5360118568972965 2204 18 3.494050351720876 2204 19 4.38754184958275 2204 20 4.769135487694296 2204 21 6.1582166185651666 2204 22 5.696019075308545 2204 23 4.427146252080426 2204 24 6.238443702324446 2204 25 6.115835510338599 2205 1 6.13495963302001 2205 2 10.510735541359068 2205 3 7.476247443193991 2205 4 8.343865724992092 2205 5 8.01446551701384 2205 6 7.364882316019376 2205 7 7.756210667598719 2205 8 7.402987093541704 2205 9 6.1525038758218 2205 10 5.305112862052237 2205 11 7.602378917889158 2205 12 6.81694738559824 2205 13 10.228345822662735 2205 14 7.501905316147333 2205 15 6.621322011389658 2205 16 6.014020420063204 2205 17 6.868335808587332 2205 18 6.665389742973833 2205 19 5.2980582502919695 2205 20 5.648605050342049 2205 21 -0.7186118388816425 2205 22 4.145542568216346 2205 23 5.088733870283736 2205 24 7.1320422543339 2205 25 7.448245380314958 2206 1 3.9266058302543634 2206 2 8.618834613411469 2206 3 6.110700530287634 2206 4 1.704518169193939 2206 5 5.796691984747948 2206 6 6.088024904568623 2206 7 6.148900047753983 2206 8 6.394747767971591 2206 9 4.808679579363773 2206 10 4.061003509796052 2206 11 3.618910637927975 2206 12 4.946128456680238 2206 13 8.164596429164433 2206 14 0.43100223511870794 2206 15 2.3083375681582154 2206 16 3.184489094250491 2206 17 4.380572738475738 2206 18 -1.3693954037995795 2206 19 0.7585226789673705 2206 20 2.2349117582508313 2206 21 3.0961056829524196 2206 22 -2.4689930031082525 2206 23 -3.1767645224867636 2206 24 0.9371057853867875 2206 25 2.469798934414907 2207 1 1.7262895064004589 2207 2 -14.028326958640932 2207 3 -6.218101913892779 2207 4 -3.8907454597350366 2207 5 1.5971240368410875 2207 6 2.2857512304284846 2207 7 3.2844270741554524 2207 8 3.570745731154581 2207 9 1.3332741447656566 2207 10 0.8901577678237338 2207 11 -0.9706174994645913 2207 12 -1.8191901045862906 2207 13 0.9247288222701151 2207 14 -4.968145233518939 2207 15 -1.0234714956774802 2207 16 0.11646017762111427 2207 17 1.1658150734086945 2207 18 1.0309147478815675 2207 19 0.49412531448513874 2207 20 0.19598309313749596 2207 21 -3.445892095065389 2207 22 -4.142931003632889 2207 23 -0.9788977796966343 2207 24 0.6592955179774016 2207 25 1.1561676908764014 2208 1 4.6949591147778875 2208 2 6.443480669883115 2208 3 6.278991535233793 2208 4 7.839110853516139 2208 5 3.3175853345926143 2208 6 5.947930616185211 2208 7 6.169029386504192 2208 8 6.354116861369231 2208 9 4.811785592239712 2208 10 4.303013981455223 2208 11 6.628420584555823 2208 12 5.971500142646331 2208 13 8.52965179281199 2208 14 2.9302324305751952 2208 15 4.392919751854002 2208 16 4.817256842119587 2208 17 5.854641260494468 2208 18 0.9117490920617257 2208 19 3.1949410239238105 2208 20 4.374221758468085 2208 21 6.92057503922781 2208 22 5.930277746491637 2208 23 1.708227547729841 2208 24 3.373089621663919 2208 25 4.8055474118240795 2209 1 3.3746407647521792 2209 2 4.93062500642457 2209 3 5.377076076139161 2209 4 0.05822995958694932 2209 5 4.890141766407339 2209 6 5.736933201511853 2209 7 6.2746642398937205 2209 8 5.142346021246702 2209 9 4.822653378490408 2209 10 3.75620001634627 2209 11 -0.20379678565281267 2209 12 3.6229843518767453 2209 13 2.8650331103871416 2209 14 4.210914898874169 2209 15 4.5004773061494845 2209 16 3.9556681335518 2209 17 1.798039987384037 2209 18 3.4489452075541323 2209 19 4.266819622198813 2209 20 4.448885557377469 2209 21 6.645450661118357 2209 22 5.138867258900483 2209 23 0.6939778355918378 2209 24 -2.69520862509356 2209 25 0.5990565763363058 2210 1 -1.923551706642435 2210 2 -14.028326958640932 2210 3 -7.721680211517683 2210 4 -1.9704504418754958 2210 5 -0.37666493305648174 2210 6 -0.8355929954132151 2210 7 -1.1085594624997341 2210 8 -21.392151795347186 2210 9 -13.264084304809101 2210 10 -6.99884471781515 2210 11 -9.856711020828946 2210 12 -4.232010633790953 2210 13 -4.84769144878593 2210 14 -3.7701775148753676 2210 15 -5.502533662208894 2210 16 -4.993653944513852 2210 17 -4.366549350645133 2210 18 -4.656508174948613 2210 19 -10.15910250166704 2210 20 -6.7245834919525045 2210 21 -5.015670496205331 2210 22 -9.98227821990903 2210 23 -7.715683637753234 2210 24 -10.308322485971503 2210 25 -7.670428645202424 2211 1 0.7666914152054218 2211 2 5.384925002998127 2211 3 1.1769877500330885 2211 4 1.8382903411404996 2211 5 1.3622356661952786 2211 6 0.577485544247601 2211 7 -21.190837943512392 2211 8 -13.197731568668894 2211 9 -8.44151706043704 2211 10 -6.78811494236508 2211 11 -9.741917495459676 2211 12 -4.54728706054145 2211 13 -6.122503010040937 2211 14 -4.208883093229783 2211 15 -4.685545087564133 2211 16 -11.704383719963921 2211 17 -7.423785697999897 2211 18 -5.316568778047188 2211 19 -8.184298350260196 2211 20 -6.118040123057156 2211 21 -3.7280454156035248 2211 22 -5.371360675753429 2211 23 -7.465006109615519 2211 24 -22.41049246788832 2211 25 -22.22015739746282 2212 1 -1.103011814887509 2212 2 -14.028326958640932 2212 3 -8.028100455791474 2212 4 -20.07714121945235 2212 5 -20.364006705208382 2212 6 -21.20629823953618 2212 7 -10.381355941756466 2212 8 -9.702950741791435 2212 9 -5.561007306014119 2212 10 -5.77497840953102 2212 11 -5.85816608991496 2212 12 -3.140927913153103 2212 13 -6.568582525791076 2212 14 -4.25218879662215 2212 15 -4.319449773070396 2212 16 -4.348775740067516 2212 17 -4.4633277181417625 2212 18 -3.7300419527143944 2212 19 -4.192094387255587 2212 20 -4.310735806647514 2212 21 -2.185804826151177 2212 22 -3.6190832914558797 2212 23 -3.6062295784041964 2212 24 -2.5018006329453897 2212 25 -2.977839574811373 2213 1 -18.784311200313322 2213 2 -14.028326958640932 2213 3 -9.084154084841467 2213 4 -6.173616173070162 2213 5 -20.364006705208382 2213 6 -11.287003248812619 2213 7 -21.190837943512392 2213 8 -11.3633948380188 2213 9 -8.990781658088732 2213 10 -7.965942719271197 2213 11 -7.028454286604763 2213 12 -5.792049148621508 2213 13 -19.486931955115043 2213 14 -12.963456207249902 2213 15 -7.8401372882392835 2213 16 -6.92544277051606 2213 17 -5.610085199967603 2213 18 -5.756999004191702 2213 19 -6.719281924414275 2213 20 -6.71174912920856 2213 21 -4.89221167654398 2213 22 -5.786686289235725 2213 23 -5.527841642497133 2213 24 -4.39750545490131 2213 25 -5.447430124735547 2214 1 -18.784311200313322 2214 2 -14.028326958640932 2214 3 -12.413363891931457 2214 4 -20.07714121945235 2214 5 -20.364006705208382 2214 6 -21.20629823953618 2214 7 -16.66073041663067 2214 8 -15.13838835448697 2214 9 -13.805975066830529 2214 10 -13.705202998162815 2214 11 -14.773013107200448 2214 12 -12.26100400329065 2214 13 -19.486931955115043 2214 14 -16.848609916827574 2214 15 -24.18336548861034 2214 16 -18.439983836209198 2214 17 -23.918990580301553 2214 18 -17.992680373513622 2214 19 -15.053638658310179 2214 20 -13.595895397686624 2214 21 -11.107237510924653 2214 22 -12.163950039310535 2214 23 -12.103744848354257 2214 24 -10.893288166813052 2214 25 -10.668544494237015 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 115785.0 3 3.0 4 638.0 5 2053.0 6 2763.0 7 2813.0 8 2500.0 9 2321.0 10 2140.0 11 1989.0 12 1918.0 13 2070.0 14 3217.0 15 6942.0 16 10016.0 17 10871.0 18 11419.0 19 12921.0 20 15707.0 21 17891.0 22 18595.0 23 19751.0 24 20848.0 25 21502.0 26 17706.0 27 14015.0 28 14670.0 29 17185.0 30 16621.0 31 19304.0 32 24941.0 33 31097.0 34 38130.0 35 44465.0 36 43997.0 37 46507.0 38 34864.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24027591360058 19.042224190178363 12.891592522381657 25.825907373839406 2 13.540331082428697 20.669857822605923 42.536399122432115 23.253411972533264 3 18.074655252888636 25.6259108848959 30.07134669668287 26.228087165532592 4 10.99563998970843 17.45725173322578 39.70025798466 31.846850292405794 5 12.222116276449121 38.23166873260924 37.0266029836256 12.519612007316036 6 30.298987513548287 38.621467532581974 18.95173726461232 12.127807689257422 7 26.265090106839196 32.03044049478198 23.721606636754995 17.982862761623824 8 23.519597234551263 38.55964051758211 21.618189240149547 16.30257300771708 9 26.17763039971321 15.427047858039074 20.653940371631716 37.741381370616 10 13.685822849247328 30.103416721208156 35.89208663612343 20.318673793421084 11 32.74864758009327 22.2965495231003 26.296727358180494 18.658075538625937 12 23.18882506095797 25.108802426238476 32.94639195577329 18.755980557030274 13 28.304294601681562 22.13742510301416 25.94272072094678 23.615559574357494 14 19.537847638410536 23.154500902759867 28.582439424054378 28.725212034775222 15 21.669009816619035 32.875521825134356 23.810300220573986 21.64516813767262 16 20.506824112634423 28.476525514406458 29.69662912699085 21.32002124596827 17 20.236052162145537 28.992126468598144 29.94986774927724 20.821953619979087 18 20.143798736341697 27.86703088807771 32.17083641567324 19.818333959907346 19 22.30880418906552 27.219532002836345 28.05907236925837 22.412591438839765 20 22.615226457529953 28.310670371750383 28.981655645688647 20.092447525031012 21 21.457359291769354 26.90719034481463 27.387099905740435 24.248350457675578 22 21.680869401603573 29.253233550016084 28.526253816226323 20.53964323215402 23 20.937416443475833 28.63186134434325 29.2262236409508 21.204498571230115 24 22.052328396257177 27.50616722822203 29.526613771774677 20.914890603746116 25 21.537818075897558 28.161564257493037 29.252152870618637 21.048464795990768 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 671.0 1 671.0 2 1525.0 3 2379.0 4 2379.0 5 2379.0 6 3333.5 7 4288.0 8 4288.0 9 4288.0 10 5210.0 11 6132.0 12 6132.0 13 6132.0 14 7676.5 15 9221.0 16 9221.0 17 9221.0 18 13217.5 19 17214.0 20 17214.0 21 17214.0 22 24034.5 23 30855.0 24 30855.0 25 30855.0 26 39923.0 27 48991.0 28 48991.0 29 48991.0 30 58871.0 31 68751.0 32 68751.0 33 68751.0 34 74284.0 35 79817.0 36 79817.0 37 79817.0 38 82071.5 39 84326.0 40 84326.0 41 84326.0 42 83199.0 43 82072.0 44 82072.0 45 82072.0 46 79296.0 47 76520.0 48 76520.0 49 76520.0 50 68996.0 51 61472.0 52 61472.0 53 61472.0 54 51805.5 55 42139.0 56 42139.0 57 42139.0 58 34579.0 59 27019.0 60 27019.0 61 27019.0 62 21161.0 63 15303.0 64 15303.0 65 15303.0 66 11516.0 67 7729.0 68 7729.0 69 7729.0 70 5510.0 71 3291.0 72 3291.0 73 3291.0 74 2304.0 75 1317.0 76 1317.0 77 1317.0 78 887.0 79 457.0 80 457.0 81 457.0 82 304.0 83 151.0 84 151.0 85 151.0 86 97.5 87 44.0 88 44.0 89 44.0 90 29.0 91 14.0 92 14.0 93 14.0 94 7.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.73118961465289 2 37.15611593986645 3 6.0123102174805085 4 14.16749356511359 5 13.930689745215801 6 15.33330846420711 7 9.707912112507927 8 7.884955422091244 9 0.10370425635095311 10 1.5110978475771255 11 6.0249934718543665 12 6.12571343305853 13 16.74816279330026 14 1.654045585108367 15 2.992203528929011 16 3.079792591487298 17 2.9686275972693696 18 1.5672025963367777 19 1.5176632969000634 20 0.2841048979744097 21 4.702204648039691 22 1.6632969000634164 23 0.10713619576976163 24 9.390532323646807 25 0.10743462528444063 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 670175.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.78019629454221 #Duplication Level Percentage of deduplicated Percentage of total 1 97.07664676232535 90.06790342224481 2 2.143477893681033 3.9774459945747624 3 0.35177464843480566 0.9791316279967454 4 0.14625292992558397 0.5427750218859042 5 0.06779426382766157 0.31449825527872116 6 0.043927729661907916 0.24453740284832406 7 0.02819600051174354 0.18312213235404046 8 0.018053331737726 0.13399933299173655 9 0.018399704173117662 0.15364153484490276 >10 0.09159770257668198 1.5701341507693773 >50 0.007104830534664328 0.4750722391072713 >100 0.006278529249334701 1.0578927458006901 >500 4.956733603602525E-4 0.2998461393027413 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 712 0.10624090722572463 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGAAC 40 8.837626E-5 19.28814 3 TAGCACT 55 6.674058E-5 15.677997 4 TGTAGGA 590 0.0 15.670658 2 TAGGACC 315 0.0 15.398033 4 GCCTTAT 70 7.9517034E-5 15.358145 1 CTGTAGG 620 0.0 15.17236 1 AGGACCT 395 0.0 14.592486 5 GGACCTG 400 0.0 14.40408 6 GTCCTAG 75 1.4311212E-4 14.334268 1 GTAGGAC 640 0.0 13.777242 3 CTAATAC 50 0.008635916 13.226152 3 TCCTATA 85 3.3015956E-4 12.987796 2 TAGGACG 505 0.0 12.592846 4 GGTTTGC 55 0.0024670598 12.445324 8 GTCCTAC 670 0.0 12.435512 1 TCCTACA 690 0.0 12.399581 2 TTAGGAC 350 0.0 12.281427 3 ATGGGTT 60 0.0028172082 12.226734 5 AGGACGT 490 0.0 12.191088 5 CTACACT 115 1.709268E-7 12.184529 4 >>END_MODULE