FastQCFastQC Report
Thu 26 May 2016
SRR1926670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926670_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670175
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14360.21427239153952327No Hit
CTGTAGGACGTGGAATATGGCAAGA14170.21143731115007275No Hit
CTTTAGGACGTGAAATATGGCGAGG13260.19785876823217816No Hit
GTCCTAAAGTGTGTATTTCTCATTT12710.18965195657850561No Hit
GTCCTACAGTGTGCATTTCTCATTT12270.18308650725556758No Hit
GTATCAACGCAGAGTACTTTTTTTT11530.1720446152124445No Hit
CTGTAGGACCTGGAATATGGCGAGA11070.16518073637482747No Hit
CCCATGTACTCTGCGTTGATACCAC9450.14100794568582833No Hit
GTACATGGGAAGCAGTGGTATCAAC8630.12877233558398926No Hit
GGTATCAACGCAGAGTACTTTTTTT6740.1005707464468236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC655.2539108E-513.2036011
TAGGACC4350.013.1530124
AGGACCT6150.013.0246915
GGCGAGG3450.012.90665719
TAAGACA1251.3224053E-912.2059964
TGGCGAG9250.012.1882218
TATGCTT550.002979812712.1366435
TGTAGGA10250.011.8232462
GTCTTAC657.763031E-411.7365341
TAATGTT657.763031E-411.7365344
TAAGGCT907.1362174E-611.6550314
CTGTAGG10100.011.6130681
GTAGGAC9750.011.5435273
GGACCTG6150.011.4724096
GTGTTAA751.9990746E-411.4431211
TTTAGGA6450.011.3916832
TTAGGAC5800.011.347033
GGCGAGA5750.011.20407819
TATGGCG10150.011.2008316
TCAGTAC600.00569744711.1277643
Produced by FastQC (version 0.11.5)