FastQCFastQC Report
Thu 26 May 2016
SRR1926669_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926669_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680145
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8890.13070742268192811No Hit
GTCCTAAAGTGTGTATTTCTCATTT8340.12262091171735438No Hit
GTCCTACAGTGTGCATTTCTCATTT7460.10968249417403642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCG250.00511289519.5484968
GTCTAGA450.001164064517.8689691
TGGACAG601.02905415E-515.6925785
GTCCTAA3350.015.602011
TAGGACC3100.014.578744
TCCTGGG1353.76167E-914.2560252
GTGTAGG852.661996E-514.1900631
TAGGACT952.2823042E-713.592214
TCCTACA7100.013.5532642
GTCTTGA600.00791207813.4017271
GCTTTAT700.001412453113.4017261
GTCCTAC6500.013.1955461
TATAGGG500.008770809513.1952363
TATAAGG853.4102835E-412.9382412
TCAAATC600.001963827812.8287013
GGACCTG3800.012.6590176
AGGACCT4150.012.6044825
TCCTAAA4300.012.46810152
CAATACT705.237716E-412.2977144
AACTGTT751.0468981E-412.2648457
Produced by FastQC (version 0.11.5)