FastQCFastQC Report
Thu 26 May 2016
SRR1926669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926669_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680145
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA19500.28670357056215956No Hit
GTCCTACAGTGGACATTTCTAAATT18490.2718537958817605No Hit
GTCCTAAAGTGTGTATTTCTCATTT18380.2702364936888457No Hit
CTTTAGGACGTGAAATATGGCGAGG18000.26464944974968574No Hit
GTCCTACAGTGTGCATTTCTCATTT15590.22921582897764448No Hit
CTGTAGGACCTGGAATATGGCGAGA13800.20289791147475905No Hit
CCCATGTACTCTGCGTTGATACCAC9750.14335178528107978No Hit
GTATCAACGCAGAGTACTTTTTTTT9240.1358533842048387No Hit
CTGAAGGACCTGGAATATGGCGAGA9150.13453013695609023No Hit
GTACATGGGAAGCAGTGGTATCAAC8710.12806092818443127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC250.006095572418.96372413
CCGTGCC453.5250967E-516.8939369
GTTGTAC400.005159857714.2974891
TAGGACC4150.013.5560474
GGCGAGG4700.013.50675819
GCCCTAC500.001460927413.3443221
TCCTATA1154.994945E-912.4408812
TGGCGAG11200.012.43579218
GTCCTAT853.7903665E-612.3350881
GTTCAGG550.002989920812.1312031
GGACCTG7000.011.8499976
TCCAACG802.9393399E-511.84361318
CATAATA657.7491277E-411.7390882
CTTTAGA657.7925617E-411.7312731
TGTAGGA11750.011.5267862
AGGACGT10400.011.4605615
CAATACT752.0006172E-411.4422234
ACTGTGC1501.7462298E-1011.4034078
GTAGGAC11400.011.3769923
AATCCCG1251.8962965E-811.36986719
Produced by FastQC (version 0.11.5)