FastQCFastQC Report
Thu 26 May 2016
SRR1926668_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926668_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714728
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8260.11556844002193842No Hit
GTCCTAAAGTGTGTATTTCTCATTT7380.10325606384526702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG757.870878E-616.140611
GTATAAG604.2763297E-415.692261
CCAAGAC601.1567336E-414.6991323
GCATTAA657.8506867E-414.4851641
CTGGACA754.02936E-614.3620024
GTATAAA954.6660443E-614.158431
GATATAC2000.014.1230341
TTCAAGG700.001119976213.8202752
TGTAGGA4850.013.6777992
TAGGACC2450.013.6292474
GTAGGAC5300.013.5204273
GTATTGG600.007725001313.4505081
TTGTAAC600.001926417112.861743
CTCTAGG957.316477E-512.7425871
ATTAACC704.270985E-412.5992563
GACGTGG2300.012.4624917
TTTAGGA4450.012.4227212
GTCCTAC6000.012.3296331
TAGGACG6400.012.2862454
CTTTAGG4000.012.1054571
Produced by FastQC (version 0.11.5)