##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926668_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 714728 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.91436322629028 27.0 7.0 30.0 2.0 31.0 2 16.223329714240943 16.0 2.0 30.0 2.0 31.0 3 19.951028922890945 23.0 15.0 31.0 2.0 31.0 4 22.168255616122497 28.0 2.0 35.0 2.0 37.0 5 22.533302179290583 29.0 2.0 35.0 2.0 37.0 6 23.384814922599926 31.0 2.0 35.0 2.0 37.0 7 23.304708924234113 30.0 2.0 35.0 2.0 37.0 8 23.513841629263162 31.0 7.0 35.0 2.0 37.0 9 26.178754715080423 31.0 17.0 37.0 2.0 39.0 10 26.820762583808104 32.0 23.0 37.0 2.0 39.0 11 23.896164694820968 30.0 7.0 37.0 2.0 39.0 12 25.016241143483956 31.0 10.0 37.0 2.0 39.0 13 21.623696007432198 27.0 2.0 35.0 2.0 39.0 14 25.05390442238166 31.0 17.0 38.0 2.0 40.0 15 26.15707653820754 31.0 17.0 38.0 2.0 40.0 16 26.53303634389586 31.0 17.0 39.0 2.0 40.0 17 25.87327067080064 31.0 11.0 38.0 2.0 40.0 18 25.971340146181486 31.0 16.0 38.0 2.0 40.0 19 26.355437313215656 31.0 17.0 39.0 2.0 40.0 20 26.573902239733158 31.0 16.0 39.0 2.0 40.0 21 24.446305447666806 30.0 2.0 37.0 2.0 40.0 22 25.369539181338915 31.0 2.0 38.0 2.0 40.0 23 25.362375617017943 31.0 2.0 38.0 2.0 40.0 24 24.38255812001209 31.0 2.0 38.0 2.0 40.0 25 24.191734478011217 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.476425595027493 1101 2 10.402506979179421 1101 3 7.073830393473919 1101 4 8.724373613820596 1101 5 8.136137107663068 1101 6 7.635286849794095 1101 7 7.3667819680529725 1101 8 6.225015133797864 1101 9 5.754728735109676 1101 10 5.338141042205976 1101 11 8.229258523857638 1101 12 5.673703980545749 1101 13 10.358324713447296 1101 14 7.60545048051377 1101 15 6.846246641102837 1101 16 6.198502999140384 1101 17 2.7612378021596946 1101 18 4.868850847758971 1101 19 5.605193808742335 1101 20 3.6768418782432555 1101 21 0.8107848574286329 1101 22 4.548008204863848 1101 23 5.603901062850468 1101 24 7.024046487741003 1101 25 7.6297993392365235 1102 1 5.877339310639684 1102 2 11.033850599751073 1102 3 7.319837914870387 1102 4 8.721525925626931 1102 5 8.531327224011875 1102 6 7.5383440928424506 1102 7 7.598967335902884 1102 8 7.6698641061323904 1102 9 6.614053926143267 1102 10 6.00356731347015 1102 11 8.620659013524538 1102 12 6.111024500693354 1102 13 10.447026390591706 1102 14 8.077981155252186 1102 15 5.842606595664009 1102 16 6.35852529521247 1102 17 7.027096588999367 1102 18 6.993722257279956 1102 19 5.742774684921521 1102 20 6.121384632372614 1102 21 5.535199761929476 1102 22 6.472940999140249 1102 23 7.032250236272986 1102 24 8.41407252334416 1102 25 8.326887443999837 1103 1 4.641032037087591 1103 2 9.369236887942442 1103 3 6.330021319851916 1103 4 7.956310224950773 1103 5 7.248073459781249 1103 6 7.004879704516423 1103 7 7.0422010818434195 1103 8 6.623375587845899 1103 9 5.48594335029339 1103 10 4.54771628411239 1103 11 5.233027148439174 1103 12 3.927237875503433 1103 13 8.242917600546122 1103 14 6.543304868284952 1103 15 5.79829488640447 1103 16 5.5277547350603875 1103 17 3.3515779496670284 1103 18 2.1364836291809013 1103 19 4.218998284901357 1103 20 4.395043298924069 1103 21 6.472558355752607 1103 22 1.910545141054083 1103 23 4.345676892282434 1103 24 5.762815619554367 1103 25 1.2680480727757697 1104 1 6.14240434655504 1104 2 11.031816320692915 1104 3 7.616134057089653 1104 4 8.257512003226285 1104 5 8.25511317403545 1104 6 7.904758381330122 1104 7 7.821969804216941 1104 8 7.94467204911156 1104 9 6.77161279127348 1104 10 5.744904640480158 1104 11 8.831487701435343 1104 12 5.554364212815827 1104 13 10.224946073316278 1104 14 8.038171964249049 1104 15 7.237306653905129 1104 16 6.998043280624454 1104 17 7.552647799582612 1104 18 5.202566587091088 1104 19 6.096822442638469 1104 20 5.058753243183276 1104 21 4.615959392514384 1104 22 6.37798301594697 1104 23 6.796602511836674 1104 24 7.863361149685787 1104 25 6.9373639118146855 1105 1 3.3321007591930467 1105 2 9.616627814871649 1105 3 6.454358759038673 1105 4 6.162796831383368 1105 5 2.2646217796191337 1105 6 5.0191545929382535 1105 7 5.963588363467004 1105 8 2.8661387071780204 1105 9 3.97696964617721 1105 10 3.6836469674815895 1105 11 7.098669305589247 1105 12 4.220754060269627 1105 13 5.396162787821606 1105 14 3.940527346367066 1105 15 4.265848596047224 1105 16 4.618061878575457 1105 17 5.237001332655787 1105 18 2.616336058500771 1105 19 2.7968176050211575 1105 20 1.3672185359666251 1105 21 2.3889703255295096 1105 22 4.084009074578891 1105 23 1.5972120516180155 1105 24 4.537200927800406 1105 25 5.449189198577891 1106 1 6.505838710399587 1106 2 11.359925029522984 1106 3 8.060390135758741 1106 4 9.416796033670146 1106 5 8.76683742809351 1106 6 7.83961660184605 1106 7 7.79925545114898 1106 8 7.397595198569366 1106 9 6.421424874522618 1106 10 5.620470074574591 1106 11 8.129614595757431 1106 12 5.235346229384831 1106 13 4.430267687110312 1106 14 5.682518970378233 1106 15 5.718308876576373 1106 16 5.585031897853526 1106 17 6.295403911928538 1106 18 6.093098007580075 1106 19 6.08231850244853 1106 20 5.65300528063192 1106 21 6.151334215710989 1106 22 6.512268730232684 1106 23 6.763894894136129 1106 24 7.70857432406445 1106 25 6.758628140278351 1107 1 3.596546613513329 1107 2 -13.896401501089262 1107 3 -5.813679054862874 1107 4 1.6515231976949032 1107 5 4.369795723050444 1107 6 4.518149411644554 1107 7 4.302140386595845 1107 8 4.825442727129481 1107 9 2.8608392911206124 1107 10 2.455541737444012 1107 11 5.208519050651027 1107 12 2.5322770872386613 1107 13 4.325894232265192 1107 14 3.6185714531290536 1107 15 3.1216853530577033 1107 16 2.9667450201570773 1107 17 3.448663885751799 1107 18 -1.7107076381936714 1107 19 1.3115045996902772 1107 20 1.662255042294305 1107 21 -1.661003755106794 1107 22 1.5874050270316893 1107 23 -1.5678781761371106 1107 24 2.0023277779400637 1107 25 2.7695389939309756 1108 1 4.753744900499939 1108 2 10.024671900647125 1108 3 0.054950965615546465 1108 4 5.491970235947743 1108 5 6.743876553135792 1108 6 6.5630713318043306 1108 7 6.735491779934637 1108 8 6.338558103068181 1108 9 5.021997656121446 1108 10 4.139915333341079 1108 11 6.433472978889689 1108 12 1.9244791643267618 1108 13 3.0053810031549624 1108 14 4.359456618365606 1108 15 4.319968590185574 1108 16 4.149239813063808 1108 17 -0.6067722071378725 1108 18 2.7635862249664775 1108 19 4.1451981957623225 1108 20 4.603876855781316 1108 21 4.222715431180603 1108 22 1.2244707823242393 1108 23 4.1714628273867795 1108 24 4.897010449895998 1108 25 5.5314187592883854 1109 1 3.2312967075519374 1109 2 -13.896401501089262 1109 3 -6.01980445370495 1109 4 1.8240860194318955 1109 5 1.5359783868025723 1109 6 2.242184233549896 1109 7 3.261376950911675 1109 8 3.4070636371075693 1109 9 2.823864361945496 1109 10 1.950977905613982 1109 11 0.6113469165927441 1109 12 1.1202584268219447 1109 13 2.1455216026300086 1109 14 2.7989309305111227 1109 15 2.8058076900552535 1109 16 2.500375988978348 1109 17 2.903401064277851 1109 18 3.1464582353921315 1109 19 1.4528880325861664 1109 20 0.489070776597746 1109 21 -0.2662073125282127 1109 22 2.1146939794021193 1109 23 2.4339993079946964 1109 24 3.4927318940691947 1109 25 3.874024438332455 1110 1 3.2455595097056786 1110 2 9.656926486079943 1110 3 6.293604871532764 1110 4 7.429882788821285 1110 5 8.360299614892845 1110 6 4.123871712069771 1110 7 6.520885485680836 1110 8 7.159469410964828 1110 9 5.240349640900703 1110 10 5.036611998866359 1110 11 2.541264432917636 1110 12 5.51569318452615 1110 13 10.083200603661055 1110 14 7.65962516810993 1110 15 6.849111619485889 1110 16 6.553847176972386 1110 17 7.03884040440845 1110 18 6.979972956436924 1110 19 6.211938324715852 1110 20 5.9885266134633675 1110 21 3.853279455715569 1110 22 6.014310201191556 1110 23 6.546010993182005 1110 24 7.364595796005272 1110 25 7.770175200160843 1111 1 4.943791516522037 1111 2 9.408285998910738 1111 3 7.283473681677108 1111 4 7.686560064282393 1111 5 8.071617402583303 1111 6 7.200292622254214 1111 7 7.195100476659185 1111 8 7.368800555712223 1111 9 5.596813905134862 1111 10 4.8300969878398945 1111 11 6.710332070247226 1111 12 5.956816254895035 1111 13 7.912703911600108 1111 14 5.723635041806805 1111 15 5.4052444633110674 1111 16 5.162595673363729 1111 17 6.466625212948944 1111 18 5.954686017784159 1111 19 4.976919905509757 1111 20 4.617058214305388 1111 21 7.341423501425275 1111 22 6.91704551594697 1111 23 6.8558846900544985 1111 24 6.691501619982816 1111 25 7.265267170890596 1112 1 -2.4126337401920246 1112 2 7.37216090017456 1112 3 5.378592411929873 1112 4 -0.7802423646275614 1112 5 3.2911782734363833 1112 6 2.4679417138813875 1112 7 5.296052046564398 1112 8 5.451104564401007 1112 9 4.03136909865777 1112 10 2.7375922978714904 1112 11 -0.9054126473673065 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3.4466480717938737 2204 4 5.789519561780306 2204 5 1.3561455249769665 2204 6 4.26072058305488 2204 7 5.273033259186292 2204 8 5.375171750457842 2204 9 3.729894858012262 2204 10 3.3474337007256345 2204 11 4.938906012073751 2204 12 5.113827957355419 2204 13 3.2228451751530685 2204 14 4.170511344976113 2204 15 4.484823148673872 2204 16 4.336607871028448 2204 17 -0.8584249590694029 2204 18 2.9299218914288616 2204 19 3.4636008635998294 2204 20 3.943691737032662 2204 21 5.338366266229276 2204 22 4.695330805771825 2204 23 3.811670761780938 2204 24 5.521921501133775 2204 25 5.540325813509444 2205 1 5.647485046775511 2205 2 10.210712153857589 2205 3 7.221036922682835 2205 4 7.60617244163317 2205 5 7.759326289541605 2205 6 7.118999183987658 2205 7 7.192114095833347 2205 8 7.093495210250243 2205 9 5.672878142665525 2205 10 5.110477583710704 2205 11 7.498652925725558 2205 12 6.5607314480675605 2205 13 9.717474455344998 2205 14 7.236711237636733 2205 15 6.119846747652851 2205 16 5.550384410076731 2205 17 6.447335428607165 2205 18 6.2865914245707515 2205 19 4.693436028772226 2205 20 5.069165609031472 2205 21 -0.9551346547480719 2205 22 3.8428235719567816 2205 23 4.865114044101922 2205 24 6.639589058658206 2205 25 7.090048701961731 2206 1 4.518083608358772 2206 2 9.137884213196454 2206 3 6.589369727595479 2206 4 2.1114899112211667 2206 5 6.405204775032285 2206 6 6.465739050825643 2206 7 6.843337083802041 2206 8 6.8653503006101815 2206 9 5.623057527583843 2206 10 4.702919054166426 2206 11 4.435737044737024 2206 12 5.626366637548095 2206 13 8.708635034049088 2206 14 0.9780291744598664 2206 15 3.3387170398416792 2206 16 4.429113530506587 2206 17 5.621934524173437 2206 18 -0.3085346454811493 2206 19 1.8692572779587344 2206 20 3.5362705867543696 2206 21 4.292558705506906 2206 22 -1.2967108616040512 2206 23 -1.8646765344352971 2206 24 2.182043711819553 2206 25 3.8092403851763095 2207 1 1.9101056320309233 2207 2 -13.896401501089262 2207 3 -6.152502943928706 2207 4 -4.2665220237628425 2207 5 1.580415615426599 2207 6 1.9462790923672983 2207 7 3.285903435059833 2207 8 3.177839832771994 2207 9 0.9097707912414883 2207 10 0.8209428581953091 2207 11 -1.0074372653747439 2207 12 -2.1617860774959183 2207 13 0.7516326272705456 2207 14 -5.365987837837782 2207 15 -1.3826808698876398 2207 16 -0.1052506513535576 2207 17 1.1267612690878792 2207 18 1.1111303836969206 2207 19 0.43080091318342184 2207 20 0.26547173409537095 2207 21 -4.026473307944677 2207 22 -4.4378489711289575 2207 23 -1.244700931916423 2207 24 0.6693161393689238 2207 25 1.2977010359956012 2208 1 4.657679867915224 2208 2 6.475889520582566 2208 3 6.24051460659971 2208 4 7.944391914127593 2208 5 3.0479791479393405 2208 6 5.928282173337806 2208 7 6.267432616752064 2208 8 6.493282947353087 2208 9 4.793200321543534 2208 10 4.315243562917296 2208 11 6.471189752135771 2208 12 5.969715345452311 2208 13 8.751471326460788 2208 14 2.991315962859435 2208 15 4.281615883589705 2208 16 4.906645595964349 2208 17 5.6903650542802175 2208 18 0.446796118403352 2208 19 2.7276044062837492 2208 20 3.9968158574942443 2208 21 6.466026829598647 2208 22 5.591605306968642 2208 23 1.037889334017347 2208 24 2.4604015812831257 2208 25 3.8472766523147435 2209 1 3.099888330247527 2209 2 4.641997191721195 2209 3 5.066709997036586 2209 4 0.00936439434775238 2209 5 4.8394738159819966 2209 6 5.4636708902280695 2209 7 5.811292265874872 2209 8 4.866175964208953 2209 9 4.33095401951395 2209 10 3.337087388166694 2209 11 -0.2334026111253209 2209 12 3.1209451356139866 2209 13 2.3760413299007617 2209 14 3.64525473134929 2209 15 4.104371301546362 2209 16 3.5726242681349696 2209 17 1.0232683910535236 2209 18 3.274324498176316 2209 19 3.7238204365555063 2209 20 3.7739056407759755 2209 21 5.839564841294553 2209 22 4.673734649933898 2209 23 0.6526984155446947 2209 24 -2.8912496055073795 2209 25 0.04266708919124795 2210 1 -1.846090568926357 2210 2 -13.896401501089262 2210 3 -7.5803654151926025 2210 4 -1.7312090770035624 2210 5 -0.4548221595994377 2210 6 -0.8243213794378335 2210 7 -0.6038565347621336 2210 8 -21.14526194428778 2210 9 -12.802485481497962 2210 10 -6.581727515121187 2210 11 -9.867273418331452 2210 12 -4.156496720993292 2210 13 -4.623913051174849 2210 14 -3.4463231857735686 2210 15 -5.2036559702760705 2210 16 -4.6002797496481165 2210 17 -4.098028181721105 2210 18 -4.253507192875741 2210 19 -9.661734123678063 2210 20 -6.382335028080398 2210 21 -4.666809366595032 2210 22 -9.602558439213775 2210 23 -7.603696528219615 2210 24 -10.623856353790448 2210 25 -8.07086597208741 2211 1 -0.08341444123021802 2211 2 4.740185865604751 2211 3 0.6989570440643114 2211 4 1.5224744920920728 2211 5 0.8197017698925748 2211 6 -0.2653426320525938 2211 7 -20.933805773340815 2211 8 -13.711299680136834 2211 9 -9.446842524315507 2211 10 -7.6602210238500135 2211 11 -9.7735834086289 2211 12 -5.174138292890035 2211 13 -6.844743278719827 2211 14 -4.866019644411406 2211 15 -5.538260074219696 2211 16 -11.710391918925032 2211 17 -7.883893270439074 2211 18 -6.000480871059157 2211 19 -8.710798639404107 2211 20 -6.734285740780749 2211 21 -4.435732466581289 2211 22 -6.016192537785596 2211 23 -8.078292914539432 2211 24 -22.106935880017183 2211 25 -21.905045329109406 2212 1 -0.410224279027922 2212 2 -13.896401501089262 2212 3 -7.599553317693168 2212 4 -19.886877435717608 2212 5 -20.140101347416696 2212 6 -21.002832377745786 2212 7 -9.825493430771546 2212 8 -9.24097982841876 2212 9 -4.768361105360519 2212 10 -5.058164583318796 2212 11 -5.119188159601638 2212 12 -2.315380822141069 2212 13 -6.102632465394015 2212 14 -3.463399225615529 2212 15 -3.2872546183430025 2212 16 -3.49922999834072 2212 17 -3.908787386757183 2212 18 -2.6978997819639545 2212 19 -3.1588700031804215 2212 20 -3.364300019321817 2212 21 -1.3495845800188917 2212 22 -2.5166433484526927 2212 23 -2.794083823799408 2212 24 -1.7929140496225564 2212 25 -2.2274634651295564 2213 1 -18.610895983477963 2213 2 -13.896401501089262 2213 3 -8.27582678210381 2213 4 -5.075923372113369 2213 5 -20.140101347416696 2213 6 -10.418910116261687 2213 7 -20.933805773340815 2213 8 -10.307806113899085 2213 9 -7.517160255642523 2213 10 -6.3883797126901385 2213 11 -5.819126299894116 2213 12 -4.319870472136767 2213 13 -19.196671088399892 2213 14 -11.854462352186127 2213 15 -6.1392370958762115 2213 16 -5.376439220629205 2213 17 -4.003828077687096 2213 18 -3.9631088143713207 2213 19 -4.999441070638653 2213 20 -5.009247549828888 2213 21 -3.238534537443986 2213 22 -4.01689330914137 2213 23 -3.809177147401332 2213 24 -2.9028722757769003 2213 25 -3.7725364951871434 2214 1 -18.610895983477963 2214 2 -13.896401501089262 2214 3 -12.406969051865968 2214 4 -19.886877435717608 2214 5 -20.140101347416696 2214 6 -21.002832377745786 2214 7 -16.67339919057218 2214 8 -15.15203832376503 2214 9 -14.012626136007441 2214 10 -13.841483814435032 2214 11 -14.294342572782782 2214 12 -12.369854394911355 2214 13 -19.196671088399892 2214 14 -16.73993435557945 2214 15 -23.822099286688932 2214 16 -18.311281988785353 2214 17 -23.610718537051053 2214 18 -17.856877692283604 2214 19 -14.924374110705152 2214 20 -13.726905816430333 2214 21 -11.280705612805123 2214 22 -12.329180585214695 2214 23 -12.241973490003586 2214 24 -11.074022036841965 2214 25 -10.813080179061004 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 130391.0 3 2.0 4 721.0 5 2302.0 6 3140.0 7 3274.0 8 2821.0 9 2577.0 10 2369.0 11 2144.0 12 2078.0 13 2334.0 14 3427.0 15 7276.0 16 11047.0 17 11579.0 18 12316.0 19 14089.0 20 16629.0 21 18740.0 22 19344.0 23 20898.0 24 21991.0 25 22582.0 26 18556.0 27 14892.0 28 15831.0 29 18082.0 30 17775.0 31 20309.0 32 25894.0 33 32848.0 34 39999.0 35 46150.0 36 45821.0 37 48295.0 38 36205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48461544714786 18.673798704201992 12.982692886117727 25.858892962532416 2 13.756221350513345 20.2731139367187 42.35142476745329 23.619239945314664 3 18.405218114875474 25.196283256311265 29.82029177422169 26.578206854591574 4 11.342434863599598 17.00858681176826 39.1349461179604 32.514032206671736 5 12.689351358572578 37.825632203008766 36.521800047572036 12.963216390846618 6 30.942281585503512 37.76502550178935 18.72114783744495 12.571545075262186 7 26.89435703666997 31.638379583746286 23.219772051536175 18.247491328047573 8 24.076569971600794 38.18044511842119 21.1974466546222 16.54553825535581 9 26.26834023338805 15.324003787093629 20.41375118346676 37.99390479605157 10 14.54438695937137 29.468232270025354 35.10110595972822 20.886274810875058 11 33.05698848239998 22.23390436648726 25.981635669215365 18.7274714818974 12 23.81320345190235 24.601740868644224 32.18787060587301 19.397185073580417 13 28.58087032759522 21.777579201159988 25.720186811889867 23.921363659354924 14 19.922601368571826 22.92343572467957 28.12207227858951 29.03189062815909 15 22.174498893008117 32.26177289119129 23.540294360190465 22.02343385561013 16 21.094774336148863 27.978530095018638 29.11404116173407 21.812654407098425 17 20.642431681166745 28.644597667954226 29.53193025522544 21.181040395653586 18 20.664352576322305 27.31940137138305 31.65206134236208 20.36418470993257 19 22.984328479171463 26.728459834440947 27.632181358539714 22.655030327847875 20 23.290740382214217 27.93457857389372 28.212919861839463 20.5617611820526 21 21.959381507842846 26.402166868255172 26.96254862741006 24.67590299649192 22 22.288709679716217 28.78039819373103 27.999162656770448 20.931729469782304 23 21.47029782704603 28.225992815176365 28.685093241643965 21.61861611613364 24 22.69751544629946 27.16429913624448 28.8271638351673 21.31102158228876 25 22.054881242839418 27.617237505917547 28.80172778432949 21.52615346691355 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 733.0 1 733.0 2 1615.5 3 2498.0 4 2498.0 5 2498.0 6 3589.5 7 4681.0 8 4681.0 9 4681.0 10 5584.0 11 6487.0 12 6487.0 13 6487.0 14 7823.0 15 9159.0 16 9159.0 17 9159.0 18 13163.5 19 17168.0 20 17168.0 21 17168.0 22 23791.0 23 30414.0 24 30414.0 25 30414.0 26 39883.0 27 49352.0 28 49352.0 29 49352.0 30 59241.5 31 69131.0 32 69131.0 33 69131.0 34 75015.0 35 80899.0 36 80899.0 37 80899.0 38 83973.5 39 87048.0 40 87048.0 41 87048.0 42 87028.5 43 87009.0 44 87009.0 45 87009.0 46 84665.0 47 82321.0 48 82321.0 49 82321.0 50 75512.0 51 68703.0 52 68703.0 53 68703.0 54 59019.5 55 49336.0 56 49336.0 57 49336.0 58 41047.0 59 32758.0 60 32758.0 61 32758.0 62 26070.0 63 19382.0 64 19382.0 65 19382.0 66 14788.5 67 10195.0 68 10195.0 69 10195.0 70 7411.5 71 4628.0 72 4628.0 73 4628.0 74 3225.0 75 1822.0 76 1822.0 77 1822.0 78 1255.5 79 689.0 80 689.0 81 689.0 82 466.5 83 244.0 84 244.0 85 244.0 86 151.0 87 58.0 88 58.0 89 58.0 90 35.0 91 12.0 92 12.0 93 12.0 94 6.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.9441857601773 2 37.36750204273514 3 6.175915872891506 4 14.375118926360797 5 14.120336687523086 6 15.591525727269676 7 9.649545001734925 8 7.920635542472102 9 0.1012972767262511 10 1.5889960936188312 11 6.182631714442417 12 6.208375773720912 13 17.01598370289117 14 1.7335266003290761 15 3.1216071008831334 16 3.213670095476881 17 3.106496457393582 18 1.6492987542113924 19 1.5969711554605386 20 0.2705924491554829 21 4.798888528223324 22 1.7498964641094235 23 0.1011573633606071 24 9.53271734142219 25 0.10493502423299494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 714728.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.172981402385 #Duplication Level Percentage of deduplicated Percentage of total 1 96.68518035985272 89.11761331194946 2 2.4209002163743643 4.462831812418083 3 0.4085461526365439 1.1297075078695233 4 0.16357939478954472 0.6031040205500041 5 0.09078936649310464 0.41841632946516244 6 0.05221272825127502 0.2887561698043533 7 0.0322141755254415 0.207849362111978 8 0.025999855889053222 0.19171873866611147 9 0.014443501029629284 0.1198170496610414 >10 0.09160722930022507 1.570782864625846 >50 0.007810375551816877 0.5222148465002393 >100 0.006091840190322468 0.9783111186665953 >500 6.248041159322399E-4 0.38887686771159763 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 826 0.11556844002193842 No Hit GTCCTAAAGTGTGTATTTCTCATTT 738 0.10325606384526702 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.3991336564399325E-4 0.0 11 0.0 0.0 0.0 1.3991336564399325E-4 0.0 12 0.0 0.0 0.0 1.3991336564399325E-4 0.0 13 0.0 0.0 0.0 1.3991336564399325E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAGG 75 7.870878E-6 16.14061 1 GTATAAG 60 4.2763297E-4 15.69226 1 CCAAGAC 60 1.1567336E-4 14.699132 3 GCATTAA 65 7.8506867E-4 14.485164 1 CTGGACA 75 4.02936E-6 14.362002 4 GTATAAA 95 4.6660443E-6 14.15843 1 GATATAC 200 0.0 14.123034 1 TTCAAGG 70 0.0011199762 13.820275 2 TGTAGGA 485 0.0 13.677799 2 TAGGACC 245 0.0 13.629247 4 GTAGGAC 530 0.0 13.520427 3 GTATTGG 60 0.0077250013 13.450508 1 TTGTAAC 60 0.0019264171 12.86174 3 CTCTAGG 95 7.316477E-5 12.742587 1 ATTAACC 70 4.270985E-4 12.599256 3 GACGTGG 230 0.0 12.462491 7 TTTAGGA 445 0.0 12.422721 2 GTCCTAC 600 0.0 12.329633 1 TAGGACG 640 0.0 12.286245 4 CTTTAGG 400 0.0 12.105457 1 >>END_MODULE