FastQCFastQC Report
Thu 26 May 2016
SRR1926668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926668_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714728
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17730.24806639728680002No Hit
CTGTAGGACGTGGAATATGGCAAGA16620.2325360137003168No Hit
CTTTAGGACGTGAAATATGGCGAGG15100.21126918212242982No Hit
GTCCTAAAGTGTGTATTTCTCATTT14650.2049730806684501No Hit
GTATCAACGCAGAGTACTTTTTTTT11640.16285915760960815No Hit
GTCCTACAGTGTGCATTTCTCATTT11140.15586348932740846No Hit
CTGTAGGACCTGGAATATGGCGAGA9530.13333743745872556No Hit
CTGAAGGACCTGGAATATGGCGAGA8690.12158471474463012No Hit
GTACATGGGAAGCAGTGGTATCAAC8280.1158482667532264No Hit
CCCATGTACTCTGCGTTGATACCAC8260.11556844002193842No Hit
GGTATCAACGCAGAGTACTTTTTTT7650.10703372471765484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG250.005893232319.0750265
TAGGACC4100.014.6552024
CTAGGAC801.2246164E-714.3062713
GATATAC2500.013.3431191
ATCCCGT655.5490753E-513.12718110
ACTGTTC2450.012.81034858
CTATAGA751.4275991E-512.7077321
TCCAACG1155.5442797E-912.35407418
TGTAGGA10400.012.2887182
CCAACGA854.069503E-612.25717919
GTAGGAC10250.012.1894073
TAGGTAG550.002976452712.1386545
TTAACCC550.002976452712.1386544
TAGGACA3350.011.9574794
AAGGTGT1052.5835288E-711.8058546
AGGACCT7850.011.7852085
ACTAGAC657.753362E-411.7384783
GTCCTAG1302.4738256E-911.7302141
GGCGAGG3650.011.67713819
CTGTAGG10650.011.633841
Produced by FastQC (version 0.11.5)