FastQCFastQC Report
Thu 26 May 2016
SRR1926667_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926667_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633261
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7040.11117059158861828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGG603.6800967E-416.0040252
GATATAC902.799683E-614.8721541
GTATTAA550.004481140514.6017521
GCCTAGG658.143696E-414.414551
TCCTACA5600.013.9626932
GTCCTAC5450.013.7533321
CCCTACA600.006779944513.7177352
GTCTAGA600.00797652313.3849391
CTAGGAC500.00884171913.1788753
CTGTAGG5000.013.117241
GACAGAT550.001783734912.9938267
CCAGGAC1056.0053935E-712.5513093
CTAGAGT600.002335567312.5358564
GTCCTAA2900.012.461841
TGTAGGA5250.012.2806392
ATACTGT954.714455E-612.0944136
TAGGACA2150.011.9944394
TAGACAG706.688287E-411.9451855
ATTTAAC650.003611808911.8271953
CAGTAGG800.003884080811.7118221
Produced by FastQC (version 0.11.5)