##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926667_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 633261 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.969931829056264 27.0 7.0 30.0 2.0 31.0 2 16.32075873928759 16.0 2.0 30.0 2.0 31.0 3 20.03469185691208 24.0 15.0 31.0 2.0 31.0 4 22.269991362171364 28.0 2.0 35.0 2.0 37.0 5 22.627755380482927 29.0 2.0 35.0 2.0 37.0 6 23.4851475142161 31.0 2.0 35.0 2.0 37.0 7 23.404612000423207 31.0 7.0 35.0 2.0 37.0 8 23.611591429126378 31.0 8.0 35.0 2.0 37.0 9 26.26776637121187 32.0 17.0 37.0 2.0 39.0 10 26.9154992964986 32.0 23.0 37.0 2.0 39.0 11 24.01931115290536 30.0 7.0 37.0 2.0 39.0 12 25.146565160336735 31.0 11.0 37.0 2.0 39.0 13 21.792022562576882 27.0 2.0 37.0 2.0 39.0 14 25.1721849284892 31.0 17.0 38.0 2.0 40.0 15 26.26322479988504 31.0 17.0 38.0 2.0 40.0 16 26.633208108505023 31.0 17.0 39.0 2.0 40.0 17 25.952458780818652 31.0 11.0 38.0 2.0 40.0 18 26.042762778696304 31.0 16.0 38.0 2.0 40.0 19 26.424313197875758 31.0 17.0 39.0 2.0 40.0 20 26.663590525865324 31.0 17.0 39.0 2.0 40.0 21 24.509488188914208 30.0 2.0 38.0 2.0 40.0 22 25.453800249818006 31.0 2.0 38.0 2.0 40.0 23 25.458164958840037 31.0 2.0 38.0 2.0 40.0 24 24.50362488768454 31.0 2.0 39.0 2.0 40.0 25 24.309472713462537 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.6183500077379485 1101 2 10.303193539944102 1101 3 6.980755406647798 1101 4 8.35575325604242 1101 5 7.926771782994983 1101 6 7.589368046726062 1101 7 7.239789234350109 1101 8 5.7124165803845415 1101 9 5.61530498949584 1101 10 5.364967317629201 1101 11 7.88092067137762 1101 12 5.545553111331337 1101 13 10.020411950536193 1101 14 7.40895725040777 1101 15 6.676606431190464 1101 16 6.206421189750735 1101 17 2.117880602209322 1101 18 4.696643877307363 1101 19 5.457893982187699 1101 20 3.5602412414814957 1101 21 0.8287077471979458 1101 22 4.520272149469644 1101 23 5.584527163398956 1101 24 7.0633126221851334 1101 25 7.171956612490028 1102 1 6.102707418881131 1102 2 11.352739192846753 1102 3 7.665028112642915 1102 4 9.048227272223844 1102 5 8.628738262733048 1102 6 7.620692662059238 1102 7 7.558538650287538 1102 8 7.864309714723646 1102 9 6.595829664156913 1102 10 6.005243929663333 1102 11 8.545210507017053 1102 12 5.8243273558222874 1102 13 10.67288164572626 1102 14 8.027494303337345 1102 15 5.7791871089859725 1102 16 6.371142695856278 1102 17 7.384856139739792 1102 18 7.299940862301348 1102 19 6.07575373686856 1102 20 6.282907327443507 1102 21 5.089534445437568 1102 22 6.250863482281144 1102 23 6.585091292826366 1102 24 7.710578266274336 1102 25 7.6242109439837975 1103 1 4.830831110857652 1103 2 9.396307146755086 1103 3 6.580152997009243 1103 4 8.028643412749972 1103 5 7.544026704993595 1103 6 7.107775105790047 1103 7 7.185445506704809 1103 8 6.804001698276405 1103 9 5.812275674223638 1103 10 4.936377262762175 1103 11 5.564557823084769 1103 12 4.4910930951919035 1103 13 8.70594760000743 1103 14 6.876523332225538 1103 15 6.003592057227813 1103 16 5.703224137135475 1103 17 3.3354590176552534 1103 18 1.892732707621306 1103 19 4.205768193275983 1103 20 4.563351958699592 1103 21 7.002306206551591 1103 22 2.5493742261002375 1103 23 4.941720487734617 1103 24 6.332446484337456 1103 25 1.8159931270771885 1104 1 5.687679764668154 1104 2 10.627237149098717 1104 3 7.223437265638498 1104 4 8.015603896307816 1104 5 7.884732278291576 1104 6 7.538261916735859 1104 7 7.3945937187230975 1104 8 7.350674089935872 1104 9 6.255404983271806 1104 10 5.1290483391890795 1104 11 8.099310045163186 1104 12 4.967282488680802 1104 13 9.28745791373937 1104 14 7.175796456357606 1104 15 6.317414941574796 1104 16 6.00886442102982 1104 17 6.3675606613461575 1104 18 4.013714864532748 1104 19 4.721578967689112 1104 20 4.083523813422364 1104 21 3.233321034399541 1104 22 5.129358555244181 1104 23 5.7882431667514105 1104 24 6.662923151202342 1104 25 6.1283022696924725 1105 1 3.3245819435675195 1105 2 9.275790542093278 1105 3 6.171123347413555 1105 4 5.572819738851113 1105 5 1.806410595345774 1105 6 4.665425958655764 1105 7 5.662662564702412 1105 8 3.287744639458907 1105 9 3.6279752017568683 1105 10 3.5746378471451514 1105 11 6.8078337976542365 1105 12 3.8025470834878448 1105 13 4.412537366985319 1105 14 3.3630052483293795 1105 15 4.217599347495952 1105 16 4.485025967559874 1105 17 5.30584956285389 1105 18 2.3334710949484005 1105 19 2.2902468229515414 1105 20 1.7439201828326354 1105 21 2.766903824061899 1105 22 4.1196224846690335 1105 23 1.83463229052159 1105 24 4.6408514734225506 1105 25 5.200777681547514 1106 1 6.155552007225257 1106 2 10.836165033118109 1106 3 7.6688054792787455 1106 4 8.898597299975926 1106 5 8.492136350922998 1106 6 7.5681949747326875 1106 7 7.686082591083096 1106 8 7.203386187125744 1106 9 5.952366821110349 1106 10 5.474369739099043 1106 11 7.94464775647743 1106 12 5.349805308335391 1106 13 4.805282200767888 1106 14 5.812454216997537 1106 15 6.051427318668363 1106 16 5.994600913555935 1106 17 6.560889133881016 1106 18 6.670508238187871 1106 19 6.292297060951196 1106 20 5.763211058556667 1106 21 6.063516869119347 1106 22 6.49531306599992 1106 23 6.7351837997643855 1106 24 7.660393252398293 1106 25 6.419500799056262 1107 1 3.9420512297382473 1107 2 -13.917381066172672 1107 3 -5.5914156304417375 1107 4 2.0078342249199856 1107 5 4.781721817604833 1107 6 4.951695183326763 1107 7 4.812618602005841 1107 8 5.2999605035060675 1107 9 3.7216902177804307 1107 10 3.1626401149476386 1107 11 5.826190025222452 1107 12 3.3593958950536376 1107 13 4.449444411106569 1107 14 4.134651471140973 1107 15 3.928058145080165 1107 16 3.698288287925415 1107 17 4.270208548300555 1107 18 -0.9325656799673965 1107 19 1.8897010128607832 1107 20 2.1710646255153705 1107 21 -1.001057219703803 1107 22 2.2212868210725496 1107 23 -0.9260689024770521 1107 24 2.878307690900968 1107 25 3.524513693962774 1108 1 4.72460278686853 1108 2 10.129191868800968 1108 3 -0.26211413831588715 1108 4 5.460454608430474 1108 5 6.761446700048623 1108 6 6.677616131670952 1108 7 6.812952239215317 1108 8 6.595365708246536 1108 9 5.1539180058440515 1108 10 4.045196684467925 1108 11 6.323249945212176 1108 12 2.193155281537102 1108 13 3.4210554872353747 1108 14 4.6605717929364125 1108 15 4.465048016919621 1108 16 4.49674321870744 1108 17 -0.11962695132508117 1108 18 3.134551788525034 1108 19 4.040265376647518 1108 20 4.570751037865897 1108 21 4.458437969968166 1108 22 1.6456608205525143 1108 23 4.3481385916725515 1108 24 5.24079747032448 1108 25 5.797320851655694 1109 1 3.7223679399318392 1109 2 -13.917381066172672 1109 3 -5.652172584386106 1109 4 2.249586909080328 1109 5 1.7457005930473564 1109 6 2.5245086808187835 1109 7 3.8582671531810675 1109 8 3.992051039509054 1109 9 3.4954032717123624 1109 10 2.6642435639382285 1109 11 0.7960884623985898 1109 12 1.3290787703179063 1109 13 2.4207473170469562 1109 14 3.141722730934532 1109 15 3.419137198265922 1109 16 3.2704882630528864 1109 17 3.915833056031765 1109 18 3.9242456619055055 1109 19 2.518533247657448 1109 20 1.6277723318537163 1109 21 0.5041427968778791 1109 22 2.961631720131539 1109 23 3.2019972584482055 1109 24 4.386672322165737 1109 25 4.614027436034654 1110 1 3.496708300722972 1110 2 9.11159089644415 1110 3 6.370339912897094 1110 4 7.60805246004751 1110 5 8.406943801045617 1110 6 3.924060952064167 1110 7 6.457493408342991 1110 8 7.124674382260629 1110 9 5.495433699880152 1110 10 5.141018178152532 1110 11 2.885651600846753 1110 12 5.681910763362897 1110 13 10.113927060580973 1110 14 7.513281087813471 1110 15 6.738285209567813 1110 16 6.335591501479314 1110 17 6.838719385090666 1110 18 6.7926829581454555 1110 19 5.605483910556817 1110 20 5.916400890862629 1110 21 3.7905848746870525 1110 22 5.924513702309326 1110 23 6.467943791810519 1110 24 7.387028766416986 1110 25 7.5892545605727975 1111 1 4.967417656424566 1111 2 9.255763810152418 1111 3 7.486513421037511 1111 4 7.893259355460476 1111 5 8.357321925942419 1111 6 7.354244895791254 1111 7 7.18285914923662 1111 8 7.447581485724847 1111 9 5.790305071367225 1111 10 5.201260574374583 1111 11 7.1627923821947945 1111 12 6.406777841728871 1111 13 8.394063119495804 1111 14 6.272403970148929 1111 15 5.574642991675407 1111 16 5.448642589620395 1111 17 6.7582646444942505 1111 18 6.52159712874748 1111 19 5.449960645142863 1111 20 5.3034868523566345 1111 21 7.70041447080343 1111 22 7.144286827371246 1111 23 6.891205903365616 1111 24 6.569403853105008 1111 25 7.545634799006141 1112 1 -2.1130650140513367 1112 2 7.433682763614561 1112 3 5.487067890626264 1112 4 -0.620906246123365 1112 5 3.4956824502137778 1112 6 2.3181949747326875 1112 7 4.920125144274586 1112 8 5.113847180033545 1112 9 4.030381005507515 1112 10 2.943341370304715 1112 11 -0.6670543711821466 1112 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1.1823770861142862 2204 6 4.467270991114667 2204 7 5.387228830220046 2204 8 5.4669937188408575 2204 9 3.8410043198617245 2204 10 3.4598282347538216 2204 11 4.930046318059688 2204 12 4.971389066201738 2204 13 2.7009394777631925 2204 14 4.149361119404887 2204 15 4.374983906421836 2204 16 4.386282563740732 2204 17 -1.4363014564695966 2204 18 2.4486173181998616 2204 19 3.541138335549139 2204 20 3.960791220378667 2204 21 5.641218896888798 2204 22 4.980004904977047 2204 23 3.666903907645718 2204 24 5.474712501224584 2204 25 5.799929827125386 2205 1 6.09493867286778 2205 2 10.597961894116137 2205 3 7.675820867045346 2205 4 8.00438499488337 2205 5 8.08965408141945 2205 6 7.554618663188023 2205 7 8.001285371634598 2205 8 7.550727892837909 2205 9 6.50766420698228 2205 10 5.787364208720366 2205 11 8.126327737018688 2205 12 7.352093630531154 2205 13 10.302123356002546 2205 14 7.691230748733208 2205 15 6.750524151502425 2205 16 6.18243856109671 2205 17 6.9831405868856 2205 18 7.132454880275077 2205 19 5.760369365531684 2205 20 5.756928573982844 2205 21 -0.7738842369563912 2205 22 4.391349627947843 2205 23 5.5717495882274015 2205 24 7.534941988860389 2205 25 7.770657441911574 2206 1 3.9774270666438127 2206 2 8.589321346695963 2206 3 5.958392496562414 2206 4 2.0807650789579206 2206 5 5.76190609214331 2206 6 6.118624689713769 2206 7 6.093553642363684 2206 8 5.945597603093649 2206 9 4.715343833260256 2206 10 3.7106155322654324 2206 11 3.526985234849075 2206 12 4.681477298904479 2206 13 7.940335820070835 2206 14 0.47041487716143493 2206 15 2.3940275696523337 2206 16 3.325526607089298 2206 17 4.323431202217108 2206 18 -1.1976538746408316 2206 19 0.9414756588634674 2206 20 2.4664439032318164 2206 21 3.1862351966534277 2206 22 -2.4427442800347237 2206 23 -3.073027369022334 2206 24 0.9559696599049943 2206 25 2.5801234104303994 2207 1 1.3741534412522256 2207 2 -13.917381066172672 2207 3 -6.455561288705043 2207 4 -4.483835869691287 2207 5 1.0667603085256765 2207 6 1.6906523046368278 2207 7 2.7454602701416277 2207 8 3.0643186943348475 2207 9 0.5531773580911015 2207 10 0.3071867447883889 2207 11 -1.7196905647757852 2207 12 -2.472938437280696 2207 13 0.467827988313676 2207 14 -5.454915824308674 2207 15 -1.7539950957966042 2207 16 -0.5940223426205122 2207 17 0.6260340953025718 2207 18 0.584525072417474 2207 19 -0.13652766042671516 2207 20 -0.2874572447712076 2207 21 -3.857465517288574 2207 22 -4.632658253460761 2207 23 -1.8101945807203776 2207 24 0.06607090807595029 2207 25 0.5888660083150867 2208 1 4.860467745297331 2208 2 6.778046026148294 2208 3 6.504441176474298 2208 4 8.149175568108763 2208 5 3.5445721142063356 2208 6 6.199787690389986 2208 7 6.755513777175288 2208 8 6.644132826133447 2208 9 5.070772575643176 2208 10 4.54060209274213 2208 11 6.318273987209473 2208 12 6.052195330303462 2208 13 8.493051829758397 2208 14 2.908993634078783 2208 15 4.476941640748414 2208 16 5.0636756539472 2208 17 5.682179344933587 2208 18 0.6410456298008427 2208 19 3.4865875045348673 2208 20 4.3100959067077405 2208 21 6.947902252703873 2208 22 5.92732066609538 2208 23 1.559844640915049 2208 24 2.966476082424176 2208 25 4.340616917745031 2209 1 2.6347288873115424 2209 2 4.634692666546224 2209 3 4.90434654539634 2209 4 0.2586963292925262 2209 5 5.007396016903321 2209 6 5.44244244997536 2209 7 5.675461451755712 2209 8 3.9175681330681584 2209 9 3.9174630723768153 2209 10 2.7598217441975805 2209 11 -0.7575199397019361 2209 12 2.8626635950955297 2209 13 1.4249826615973795 2209 14 3.100123759763168 2209 15 3.8099420423340824 2209 16 3.364201691409974 2209 17 0.803412093648312 2209 18 2.949632008492152 2209 19 3.534132861846711 2209 20 3.672320611780844 2209 21 5.940100357696657 2209 22 4.77401195804816 2209 23 0.3640934967935081 2209 24 -3.407579097832123 2209 25 -0.11683527964426332 2210 1 -2.3429162607744978 2210 2 -13.917381066172672 2210 3 -7.857048531166496 2210 4 -2.3711145413539114 2210 5 -0.9951119166703286 2210 6 -1.5871272224263677 2210 7 -1.4842698850899616 2210 8 -21.189344309328156 2210 9 -13.45456585380684 2210 10 -7.528274594254615 2210 11 -10.331645706100556 2210 12 -4.986136795751261 2210 13 -5.66260223094176 2210 14 -4.3620235208146525 2210 15 -6.036130343547896 2210 16 -5.446896068703406 2210 17 -4.889386730290173 2210 18 -4.913312810807231 2210 19 -10.6447825383091 2210 20 -7.3979561059864025 2210 21 -5.629036830407163 2210 22 -10.181072351301792 2210 23 -8.10483022206089 2210 24 -10.698011714172862 2210 25 -8.468149325920876 2211 1 -0.003293465967839637 2211 2 4.532524415868915 2211 3 0.48894316714156716 2211 4 1.3562954860355312 2211 5 0.8566673139336274 2211 6 -0.061907989511368555 2211 7 -20.976151451470095 2211 8 -13.321669451105095 2211 9 -9.04248831838743 2211 10 -7.192177942194448 2211 11 -9.853031861160561 2211 12 -4.745776815034006 2211 13 -6.347128007171621 2211 14 -4.699931992765297 2211 15 -5.086476728845344 2211 16 -11.616992283832419 2211 17 -7.490158942913148 2211 18 -5.904700572762803 2211 19 -8.422557006002375 2211 20 -6.548569163728118 2211 21 -4.187845597196063 2211 22 -5.710424375177734 2211 23 -7.4527158905384745 2211 24 -22.089606747601707 2211 25 -21.884577215128136 2212 1 -1.2945261414084008 2212 2 -13.917381066172672 2212 3 -8.096387287350947 2212 4 -19.862040997896415 2212 5 -20.16034591858055 2212 6 -20.979677365692844 2212 7 -10.450085100759194 2212 8 -9.742898811697824 2212 9 -5.59736428036523 2212 10 -6.039457527211333 2212 11 -6.112603260295117 2212 12 -3.506825561215212 2212 13 -6.87327230160178 2212 14 -4.1006755937651285 2212 15 -4.220717315125459 2212 16 -4.398097651198189 2212 17 -4.507907713283785 2212 18 -3.5450324159077873 2212 19 -4.242463115177337 2212 20 -4.204142139655154 2212 21 -2.0807177784555257 2212 22 -3.460041375867579 2212 23 -3.707717279190941 2212 24 -2.5009811551846433 2212 25 -3.096899490009651 2213 1 -18.586469269370486 2213 2 -13.917381066172672 2213 3 -8.992560260955813 2213 4 -5.9720101654195386 2213 5 -20.16034591858055 2213 6 -11.027981579464683 2213 7 -20.976151451470095 2213 8 -11.123568358660119 2213 9 -8.588395098229558 2213 10 -7.744352243800961 2213 11 -6.885942178869126 2213 12 -5.529280177075673 2213 13 -19.319717799232112 2213 14 -12.610409012175964 2213 15 -7.339996624244176 2213 16 -6.79603435938073 2213 17 -5.230351126190563 2213 18 -5.360595753998318 2213 19 -6.190655786497288 2213 20 -6.230825626988512 2213 21 -4.4113169416435944 2213 22 -5.4229644700259705 2213 23 -5.073561908835906 2213 24 -4.157438196728119 2213 25 -5.033600853360408 2214 1 -18.586469269370486 2214 2 -13.917381066172672 2214 3 -12.425427360133614 2214 4 -19.862040997896415 2214 5 -20.16034591858055 2214 6 -20.979677365692844 2214 7 -16.735080022898664 2214 8 -15.30318359504244 2214 9 -14.065126213337471 2214 10 -14.033157806493664 2214 11 -14.631807094079816 2214 12 -12.454423098452857 2214 13 -19.319717799232112 2214 14 -16.868863855444207 2214 15 -23.87853722033873 2214 16 -18.387720831631498 2214 17 -23.581841362572884 2214 18 -17.986960733443333 2214 19 -15.179310398521954 2214 20 -13.787400010338972 2214 21 -11.368568997142052 2214 22 -12.461183894486402 2214 23 -12.381766238229535 2214 24 -11.182240676173135 2214 25 -10.971630786556707 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 114614.0 3 5.0 4 640.0 5 2182.0 6 2794.0 7 2744.0 8 2402.0 9 2219.0 10 2146.0 11 1933.0 12 1846.0 13 2053.0 14 3068.0 15 6348.0 16 9394.0 17 10229.0 18 10534.0 19 11914.0 20 14395.0 21 16466.0 22 17244.0 23 18451.0 24 19581.0 25 20048.0 26 16522.0 27 13166.0 28 13760.0 29 16430.0 30 15559.0 31 18101.0 32 23149.0 33 29420.0 34 35787.0 35 41760.0 36 40818.0 37 43379.0 38 32160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26410133275877 18.825696918349124 13.262949160155642 25.647252588736464 2 13.84614615265366 20.294895708834872 42.002260350354305 23.856697788157163 3 18.26218678548003 25.559411994625215 29.850180417602584 26.32822080229217 4 11.495938275756256 17.21597789157833 38.747250687672604 32.54083314499281 5 12.79236957929513 37.81990070501634 36.198399719017885 13.189329996670654 6 30.98740747360112 37.50204786798326 18.69331129082704 12.81723336758858 7 26.719649649300347 31.858533691593678 23.15106256761001 18.27075409149597 8 24.213101172217353 37.870768514461 21.14458945004382 16.771540863277824 9 26.563594255135836 15.361425308071109 20.40976026179128 37.66522017500178 10 14.830490811906206 29.32991525342209 34.74385242616338 21.095741508508326 11 32.63598747596372 22.41641413887183 25.903479010829333 19.04411937433512 12 24.01197463877739 24.734924124762152 31.922060632954796 19.33104060350566 13 28.29492773771087 21.976209525646915 25.654483931310633 24.074378805331577 14 20.149016510297262 23.015200262007053 27.97599553046795 28.859787697227734 15 22.099973784521794 32.12926673190684 23.61949454604073 22.15126493753063 16 21.08571223921027 28.02252363712135 29.075938322089968 21.815825801578413 17 20.8541883355226 28.648953953782875 29.247787538597013 21.24907017209751 18 20.844650583737494 27.191883345553023 31.557761695205123 20.405704375504364 19 22.928043919536435 26.797138303569422 27.614697139265594 22.66012063762855 20 23.366002980333285 27.716624463753796 28.284344698823226 20.633027857089694 21 21.914303456841232 26.319022976609396 26.99532187952805 24.77135168702132 22 22.481521388300546 28.633618467852916 27.784156326726585 21.100703817119953 23 21.293440474515503 28.2971918913749 28.72481863409221 21.684549000017387 24 22.650612044791142 27.18205572641311 28.8393836610727 21.32794856772305 25 22.118216554870674 27.48632126105247 28.71009610292293 21.68536608115393 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 670.0 1 670.0 2 1406.0 3 2142.0 4 2142.0 5 2142.0 6 3121.5 7 4101.0 8 4101.0 9 4101.0 10 4817.0 11 5533.0 12 5533.0 13 5533.0 14 6845.0 15 8157.0 16 8157.0 17 8157.0 18 11605.5 19 15054.0 20 15054.0 21 15054.0 22 20962.0 23 26870.0 24 26870.0 25 26870.0 26 35081.0 27 43292.0 28 43292.0 29 43292.0 30 51696.0 31 60100.0 32 60100.0 33 60100.0 34 65566.0 35 71032.0 36 71032.0 37 71032.0 38 73475.0 39 75918.0 40 75918.0 41 75918.0 42 76322.0 43 76726.0 44 76726.0 45 76726.0 46 75412.5 47 74099.0 48 74099.0 49 74099.0 50 67740.0 51 61381.0 52 61381.0 53 61381.0 54 52731.5 55 44082.0 56 44082.0 57 44082.0 58 36880.5 59 29679.0 60 29679.0 61 29679.0 62 23926.0 63 18173.0 64 18173.0 65 18173.0 66 13753.0 67 9333.0 68 9333.0 69 9333.0 70 6831.5 71 4330.0 72 4330.0 73 4330.0 74 3019.0 75 1708.0 76 1708.0 77 1708.0 78 1145.0 79 582.0 80 582.0 81 582.0 82 403.0 83 224.0 84 224.0 85 224.0 86 140.5 87 57.0 88 57.0 89 57.0 90 37.0 91 17.0 92 17.0 93 17.0 94 9.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.517333295434266 2 36.844681734703386 3 5.865512008476757 4 14.059605754973067 5 13.676351456982191 6 15.178101920061396 7 9.493873774004715 8 7.748621816281124 9 0.10990728941147487 10 1.5031716780284907 11 5.888093534893195 12 6.053901945643266 13 16.511675280808387 14 1.6386608365271191 15 3.019292203372701 16 2.996079025867691 17 2.984551393501258 18 1.5712320828220907 19 1.5120147932684944 20 0.28234803659154756 21 4.6404247221919555 22 1.6602948863106999 23 0.11053894050004659 24 9.168099725073864 25 0.11211806822147583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 633261.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.70050669836827 #Duplication Level Percentage of deduplicated Percentage of total 1 96.36192062682343 88.36436947907656 2 2.623792233938613 4.812061546468289 3 0.49104307377044837 1.3508669602642305 4 0.19468215791838225 0.7140981010498961 5 0.09053283869105301 0.4150953590405601 6 0.05804182658533175 0.31934789445442463 7 0.03143643304190541 0.20179157871124367 8 0.023375929335918675 0.17148676517191952 9 0.015504751655677908 0.12796142247522257 >10 0.09440408715974788 1.6117343134025877 >50 0.00816560392844831 0.5352205866007163 >100 0.006745415293725817 1.1596334717704784 >500 3.550218572708614E-4 0.21633252151387594 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 704 0.11117059158861828 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.579127721429237E-4 0.0 5 0.0 0.0 0.0 1.579127721429237E-4 0.0 6 0.0 0.0 0.0 1.579127721429237E-4 0.0 7 0.0 0.0 0.0 1.579127721429237E-4 0.0 8 0.0 0.0 0.0 1.579127721429237E-4 0.0 9 0.0 0.0 0.0 1.579127721429237E-4 0.0 10 0.0 0.0 0.0 1.579127721429237E-4 0.0 11 0.0 0.0 0.0 1.579127721429237E-4 0.0 12 0.0 0.0 0.0 1.579127721429237E-4 0.0 13 0.0 0.0 0.0 1.579127721429237E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTAGG 60 3.6800967E-4 16.004025 2 GATATAC 90 2.799683E-6 14.872154 1 GTATTAA 55 0.0044811405 14.601752 1 GCCTAGG 65 8.143696E-4 14.41455 1 TCCTACA 560 0.0 13.962693 2 GTCCTAC 545 0.0 13.753332 1 CCCTACA 60 0.0067799445 13.717735 2 GTCTAGA 60 0.007976523 13.384939 1 CTAGGAC 50 0.008841719 13.178875 3 CTGTAGG 500 0.0 13.11724 1 GACAGAT 55 0.0017837349 12.993826 7 CCAGGAC 105 6.0053935E-7 12.551309 3 CTAGAGT 60 0.0023355673 12.535856 4 GTCCTAA 290 0.0 12.46184 1 TGTAGGA 525 0.0 12.280639 2 ATACTGT 95 4.714455E-6 12.094413 6 TAGGACA 215 0.0 11.994439 4 TAGACAG 70 6.688287E-4 11.945185 5 ATTTAAC 65 0.0036118089 11.827195 3 CAGTAGG 80 0.0038840808 11.711822 1 >>END_MODULE