FastQCFastQC Report
Thu 26 May 2016
SRR1926667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926667_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633261
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA14900.23529003049295633No Hit
CTTTAGGACGTGAAATATGGCGAGG13780.21760380001294882No Hit
GTCCTACAGTGGACATTTCTAAATT13430.21207685298794654No Hit
GTCCTAAAGTGTGTATTTCTCATTT13290.20986607417794562No Hit
GTATCAACGCAGAGTACTTTTTTTT12990.20512869101365788No Hit
CCCATGTACTCTGCGTTGATACCAC12010.18965323934365133No Hit
GTACATGGGAAGCAGTGGTATCAAC11150.17607274093935993No Hit
GTCCTACAGTGTGCATTTCTCATTT10040.15854442323149537No Hit
GGTATCAACGCAGAGTACTTTTTTT8090.12775143266362526No Hit
CTGTAGGACCTGGAATATGGCGAGA8050.12711978157505358No Hit
TATCAACGCAGAGTACTTTTTTTTT6900.10895981277861735No Hit
CTGAAGGACCTGGAATATGGCGAGA6530.10311704020932917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATCG307.821984E-418.96142812
ACTGACC350.002165624616.2887028
TAGGACC2350.013.3948694
GTCCTAT801.9116887E-613.1116391
GTATTAA905.148013E-712.7143161
TGTAGGA8050.012.2107122
GATATAC1800.012.1845531
TAGGCAG550.002972866212.1402535
TGCACTC550.002972866212.1402535
CTGTAGG7700.011.8887111
GTAGGAC7950.011.7622253
TAAGGGA657.7426276E-411.74002554
CCTTTAA1302.4519977E-911.7362921
TAGGACA3300.011.5621474
GCCATAT2100.011.3520681
TTTAATC855.078611E-511.2256543
TTAATCC855.0951072E-511.2220824
TAATCTG600.005693793311.1285665
AGGACGT9200.011.0940055
GGACGTG8950.010.9775796
Produced by FastQC (version 0.11.5)