FastQCFastQC Report
Thu 26 May 2016
SRR1926666_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926666_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693015
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12560.18123705836093013No Hit
GTCCTAAAGTGTGTATTTCTCATTT11730.169260405618926No Hit
CTGTAGGACGTGGAATATGGCAAGA8960.12929013080524954No Hit
CTTTAGGACGTGAAATATGGCGAGG8370.12077660656695742No Hit
GTCCTACAGTGTGCATTTCTCATTT7980.11514902274842537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT501.09377215E-418.7585071
CCAACGT300.001633358416.99046719
TGTAGGA7750.016.312492
CAAGGCT400.002311590616.1292924
CCTAGGG706.5729815E-515.7045722
GTCCTAT604.4027992E-415.6320891
CTGTAGG7700.015.4870791
GCTGACT455.4567016E-415.1985158
TAGGACC3400.015.180514
ATAAGAC450.00437481314.6560543
GTTGTGA550.004451111414.6170181
GATATAC1750.014.5474131
GTACTAA658.081854E-414.4296221
GCCTAGG658.081854E-414.4296221
TAATTCC450.005063145414.3371484
TTTAGGA5600.014.2322682
TCTATAG857.624367E-714.2249943
GTATTAT852.6674714E-514.1871061
GTCCTAG954.854741E-614.104141
CTTTAGG5650.013.9918071
Produced by FastQC (version 0.11.5)