##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926666_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 693015 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.78583436144961 27.0 2.0 30.0 2.0 31.0 2 16.186584705958744 16.0 2.0 30.0 2.0 31.0 3 19.864399760466945 23.0 15.0 31.0 2.0 31.0 4 22.05810985332208 28.0 2.0 35.0 2.0 37.0 5 22.393437371485465 28.0 2.0 35.0 2.0 37.0 6 23.267429997907694 30.0 2.0 35.0 2.0 37.0 7 23.181075445697424 30.0 2.0 35.0 2.0 37.0 8 23.392098295130697 31.0 7.0 35.0 2.0 37.0 9 26.01897072934929 31.0 17.0 37.0 2.0 39.0 10 26.66131613312843 31.0 22.0 37.0 2.0 39.0 11 23.787544281148314 30.0 7.0 37.0 2.0 39.0 12 24.901836179592074 31.0 10.0 37.0 2.0 39.0 13 21.547040107356985 27.0 2.0 35.0 2.0 39.0 14 24.907737927750482 30.0 13.0 38.0 2.0 40.0 15 26.014755813366232 31.0 17.0 38.0 2.0 40.0 16 26.39531900463915 31.0 16.0 39.0 2.0 40.0 17 25.712371305094408 31.0 10.0 38.0 2.0 40.0 18 25.80071138431347 31.0 11.0 38.0 2.0 40.0 19 26.195997200637795 31.0 12.0 39.0 2.0 40.0 20 26.425220233328282 31.0 10.0 39.0 2.0 40.0 21 24.292697849252903 30.0 2.0 37.0 2.0 40.0 22 25.229642937021566 31.0 2.0 38.0 2.0 40.0 23 25.235198372329602 31.0 2.0 38.0 2.0 40.0 24 24.26901437919814 31.0 2.0 39.0 2.0 40.0 25 24.08175147724075 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.733039997738672 1101 2 10.578665084931897 1101 3 7.19708969280531 1101 4 8.671729133156202 1101 5 8.056117642996647 1101 6 7.900687685346917 1101 7 7.788984132628716 1101 8 6.07377560269979 1101 9 6.208794597616745 1101 10 5.582158447506632 1101 11 8.250527554220639 1101 12 5.827560213044968 1101 13 10.454389611037563 1101 14 7.902440568647254 1101 15 7.162083195686943 1101 16 6.909597442110957 1101 17 2.5800162986551953 1101 18 5.04974586381045 1101 19 5.874691582790302 1101 20 3.7919439391753933 1101 21 0.8537076053867985 1101 22 4.774802262479149 1101 23 5.86049649711077 1101 24 7.427198199090604 1101 25 8.059276353811597 1102 1 5.79489102507344 1102 2 10.811982847057367 1102 3 7.1877389391831805 1102 4 8.721474032977376 1102 5 8.414476826968034 1102 6 7.293395980664606 1102 7 7.45409489500965 1102 8 7.427918882142148 1102 9 6.565205677504338 1102 10 5.592322310139384 1102 11 8.192768348352239 1102 12 5.733968738281273 1102 13 10.043910431445578 1102 14 7.574514580544626 1102 15 5.555947653188497 1102 16 5.881660505502804 1102 17 7.04178119774647 1102 18 6.923465071138651 1102 19 5.606710122259663 1102 20 6.088760843667934 1102 21 4.996252767972106 1102 22 6.252416218174197 1102 23 6.597125087308211 1102 24 7.999215278759227 1102 25 7.804369598583687 1103 1 4.798067306074611 1103 2 9.281428283960933 1103 3 6.479020585245408 1103 4 8.019183849131121 1103 5 7.147469083810197 1103 6 6.750122209703747 1103 7 7.191783007194264 1103 8 6.588781763166146 1103 9 5.6073690473124 1103 10 4.957873337445765 1103 11 5.282770883446993 1103 12 4.319992953624972 1103 13 8.581237107313697 1103 14 6.895765197640063 1103 15 6.025083986433373 1103 16 5.644127315775421 1103 17 3.3586124479039867 1103 18 2.1461271874831915 1103 19 4.187627826560874 1103 20 4.639979394038484 1103 21 6.969019030753209 1103 22 2.539534971797085 1103 23 4.7695871387187445 1103 24 6.1644011634702345 1103 25 1.6500779500160156 1104 1 5.559632136216855 1104 2 10.528924731524034 1104 3 7.217100511413317 1104 4 7.641501942388082 1104 5 7.740088072134764 1104 6 7.180114299122611 1104 7 7.474144608647467 1104 8 7.321265685642452 1104 9 6.140821283516495 1104 10 5.0937163270594645 1104 11 8.17837465122749 1104 12 5.1736655141628916 1104 13 9.331987880060282 1104 14 7.28971067322713 1104 15 6.398502236437036 1104 16 6.023427229344996 1104 17 6.30995138700716 1104 18 4.156828445851559 1104 19 4.789412102083485 1104 20 3.916949348479399 1104 21 3.156246372065489 1104 22 5.012703452526029 1104 23 5.417413193829127 1104 24 6.667324416184005 1104 25 5.655237406822778 1105 1 3.434981152141372 1105 2 9.483434543816797 1105 3 6.476821725604495 1105 4 5.559774580393462 1105 5 2.5475301874345426 1105 6 4.759222440692863 1105 7 5.829257816463148 1105 8 3.073878502104904 1105 9 3.8398044766742174 1105 10 3.318550179825266 1105 11 6.5368001304573085 1105 12 4.053331511971315 1105 13 4.9961285395260475 1105 14 3.975935039590677 1105 15 4.228513336637693 1105 16 4.573763081570021 1105 17 5.250070785319362 1105 18 2.724425821650275 1105 19 2.4879448832529896 1105 20 1.7085018111585306 1105 21 2.6036541905914383 1105 22 3.8743109892776566 1105 23 1.139016603583073 1105 24 4.4210828302784115 1105 25 5.019770842619501 1106 1 6.587420668729422 1106 2 11.184066577964993 1106 3 7.9083035252149685 1106 4 9.237865604073527 1106 5 8.621207941778096 1106 6 7.749066153501957 1106 7 7.738787450868543 1106 8 7.359167305351935 1106 9 6.425432650729562 1106 10 5.673663543749576 1106 11 8.020141708314242 1106 12 5.419668466685767 1106 13 4.676701889224244 1106 14 5.881094041610066 1106 15 6.087461611393515 1106 16 6.05970496147318 1106 17 6.725223033038663 1106 18 6.656814518856869 1106 19 6.457690586269386 1106 20 5.7576412415757865 1106 21 5.951788341063999 1106 22 6.560732086427123 1106 23 6.896452370465202 1106 24 7.668883543239975 1106 25 6.581351914573151 1107 1 4.36061543530251 1107 2 -13.760058319323422 1107 3 -5.3534246502862874 1107 4 2.3128883527258246 1107 5 5.031641767436167 1107 6 5.368232653579177 1107 7 5.200607490141309 1107 8 5.756780986769179 1107 9 4.094513137166285 1107 10 3.6129913336101964 1107 11 6.422279713907635 1107 12 3.5181753126762203 1107 13 5.359769871758093 1107 14 4.61454000946577 1107 15 4.313852321251755 1107 16 4.110123730565505 1107 17 4.795226034991604 1107 18 -0.6984555800203758 1107 19 2.133274412629472 1107 20 2.6548153274343136 1107 21 -0.863335484480416 1107 22 2.382298410532403 1107 23 -0.6020369585615448 1107 24 2.833356466493612 1107 25 3.6697951980544516 1108 1 5.526647517505207 1108 2 10.677740723738777 1108 3 0.5236301251240185 1108 4 6.1770275823740235 1108 5 7.649693943515835 1108 6 7.633725793189058 1108 7 7.703921863803849 1108 8 7.04555000970376 1108 9 5.883838881440425 1108 10 5.0734543856110825 1108 11 7.187833197438934 1108 12 3.049081133334088 1108 13 3.883849042982998 1108 14 5.381757817281411 1108 15 5.415987976982546 1108 16 5.181561200636864 1108 17 0.466068760564319 1108 18 4.091410664109066 1108 19 5.126405587337452 1108 20 5.3278297853188015 1108 21 5.12542432465111 1108 22 1.923310864739154 1108 23 4.737175040665015 1108 24 5.84818160538196 1108 25 6.176898261579083 1109 1 3.260563007787418 1109 2 -13.760058319323422 1109 3 -5.877379448211531 1109 4 1.7574669505892828 1109 5 1.0996070750928055 1109 6 2.0410674611419317 1109 7 2.9940525028309217 1109 8 3.2361084608734174 1109 9 2.594553181288852 1109 10 1.9281803048531323 1109 11 0.3263299581767818 1109 12 0.9840109460228135 1109 13 2.147895409745832 1109 14 2.7074491039234623 1109 15 2.7581347945113635 1109 16 2.7211526222316813 1109 17 3.202182674946485 1109 18 3.361599162650709 1109 19 1.5192883129875803 1109 20 0.6260575903377763 1109 21 0.24557779879440034 1109 22 2.245206852169943 1109 23 2.5676094619313545 1109 24 3.6619151270951065 1109 25 3.865224649396133 1110 1 3.2176253791735547 1110 2 8.956372039308773 1110 3 6.31530593683846 1110 4 7.398162153581026 1110 5 8.435941254871793 1110 6 3.9192634488377713 1110 7 6.405288499743154 1110 8 7.2165477709960655 1110 9 5.558918992058924 1110 10 5.2877996733508645 1110 11 3.278822764768435 1110 12 5.661872494556157 1110 13 9.998440149606939 1110 14 7.375583081024587 1110 15 6.695592325635662 1110 16 6.456329648027236 1110 17 6.980810577575571 1110 18 7.035830721759446 1110 19 5.931886763171114 1110 20 6.010818533252021 1110 21 3.8968932388047186 1110 22 6.10363657476066 1110 23 6.637457439740267 1110 24 7.821756075334992 1110 25 8.297744580880256 1111 1 4.956860026665083 1111 2 9.377533818268715 1111 3 7.447570812222601 1111 4 8.15137992514742 1111 5 8.520313366766839 1111 6 7.739226030268242 1111 7 7.482597668795442 1111 8 7.735816831549531 1111 9 6.171351274215976 1111 10 5.737664049820744 1111 11 7.450452275847489 1111 12 6.695143463606943 1111 13 8.426221559039725 1111 14 6.464606215919282 1111 15 5.976960432691843 1111 16 5.7178989738167445 1111 17 7.1885136935355725 1111 18 6.865215569492918 1111 19 5.702378689626343 1111 20 5.309272950972492 1111 21 8.162602651303125 1111 22 7.476567470627334 1111 23 7.3794872092929715 1111 24 7.41066274681047 1111 25 7.876109434474468 1112 1 -1.8957346311884073 1112 2 7.70091127804716 1112 3 5.993759564656216 1112 4 -0.006998816633128513 1112 5 3.8348480403812175 1112 6 2.715376753337619 1112 7 5.570340450046853 1112 8 5.70920838998628 1112 9 4.509245304796941 1112 10 3.1699659266583673 1112 11 -0.3414030739371974 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3.07884772439839 2204 4 5.8264440047058805 2204 5 0.8821276836373357 2204 6 3.9938560039993405 2204 7 5.401808936321316 2204 8 5.348505185810918 2204 9 3.930618831236341 2204 10 3.516213873314392 2204 11 5.094352560197212 2204 12 5.147132106606822 2204 13 3.05401152390834 2204 14 4.52411437655104 2204 15 4.677633683926892 2204 16 4.492874074634543 2204 17 -0.41148262556187376 2204 18 3.0130243352792156 2204 19 3.901543124745835 2204 20 4.225113361194925 2204 21 5.684662937408351 2204 22 5.173069976118608 2204 23 3.8983684697472683 2204 24 5.612110757247244 2204 25 5.669092643188012 2205 1 5.8900868488735405 2205 2 10.30171129670329 2205 3 7.31763954539651 2205 4 7.912402596019842 2205 5 8.201686498891224 2205 6 7.445702426227108 2205 7 7.4337679922529105 2205 8 7.304306629588577 2205 9 6.12251817947303 2205 10 5.61549414885285 2205 11 7.715096871877726 2205 12 7.146140826123503 2205 13 10.199634522769884 2205 14 7.583327152670268 2205 15 6.496375811038771 2205 16 6.109487329511943 2205 17 6.9335118408709455 2205 18 6.794190150387365 2205 19 5.335712433143726 2205 20 5.985874958960146 2205 21 -0.2635040594446565 2205 22 4.140351227065519 2205 23 5.62789535336055 2205 24 7.2230288388092845 2205 25 7.735968003014172 2206 1 4.081659172516861 2206 2 8.522532773793985 2206 3 5.935027499679286 2206 4 1.8045088730132086 2206 5 5.623746207041783 2206 6 5.999214690256903 2206 7 6.15406295078704 2206 8 6.056333962592976 2206 9 4.678833360645431 2206 10 3.888765390395772 2206 11 3.2694060105906964 2206 12 4.527844475255325 2206 13 7.887419157079428 2206 14 0.22531075557118996 2206 15 2.347260213517938 2206 16 3.3118887678065363 2206 17 4.42688338453684 2206 18 -1.3694530335967343 2206 19 0.8588555934716702 2206 20 2.377431061235864 2206 21 3.5516419666582273 2206 22 -2.024646441112889 2206 23 -2.6010902028633858 2206 24 1.1439489958861913 2206 25 2.7668136093891675 2207 1 1.5389193587244172 2207 2 -13.760058319323422 2207 3 -6.216889102237314 2207 4 -4.349598325830829 2207 5 0.9645530610065336 2207 6 1.7411597822019935 2207 7 2.9956106818084542 2207 8 3.08564393137663 2207 9 0.8788769817416835 2207 10 0.5313668435235392 2207 11 -1.8834498080545963 2207 12 -2.4528681844047036 2207 13 0.7268403596585244 2207 14 -5.399962898649832 2207 15 -1.4460534903220825 2207 16 -0.41293640701863765 2207 17 0.7398149948368697 2207 18 0.7636594679368187 2207 19 0.37095635820401185 2207 20 0.12470201640155665 2207 21 -3.4892310005305305 2207 22 -3.872099378039511 2207 23 -1.151270821465058 2207 24 0.4580965442442668 2207 25 0.9660175243825577 2208 1 5.04490455240677 2208 2 6.4915189677428256 2208 3 6.484942568837258 2208 4 8.26397961976604 2208 5 3.873612105712265 2208 6 6.298996296663113 2208 7 6.756099375262448 2208 8 6.823548741836646 2208 9 5.436493100871999 2208 10 4.7153385267065815 2208 11 6.676858851623148 2208 12 6.18349014122807 2208 13 8.840609491566457 2208 14 3.4634849340409026 2208 15 4.879847475168791 2208 16 5.379578648903358 2208 17 6.292173490413031 2208 18 1.085115351695542 2208 19 3.4192388678429886 2208 20 4.513882995487901 2208 21 7.1238344595380845 2208 22 6.125792261807966 2208 23 2.155928405292528 2208 24 3.541780385814544 2208 25 4.873908208298477 2209 1 3.117104146143813 2209 2 4.4812062397447985 2209 3 5.359909107256403 2209 4 0.5136528772706228 2209 5 5.199594826414355 2209 6 5.854353561369152 2209 7 6.271881431456908 2209 8 5.159638172176194 2209 9 4.723165681271649 2209 10 3.6183038788798783 2209 11 -0.09635954883455256 2209 12 3.309576002332328 2209 13 2.065750817537367 2209 14 3.800181921711296 2209 15 4.243000107217373 2209 16 3.971763335834183 2209 17 1.4999384142780947 2209 18 3.54120194764236 2209 19 4.235256598544186 2209 20 4.364934345236215 2209 21 6.262507123919743 2209 22 4.943110401396556 2209 23 0.570374360246678 2209 24 -2.7823822873327337 2209 25 0.5024242987261047 2210 1 -2.2335060703089447 2210 2 -13.760058319323422 2210 3 -7.822207335969646 2210 4 -2.1288482405433022 2210 5 -0.9700328865750087 2210 6 -1.457715164029782 2210 7 -1.349266874963373 2210 8 -20.918564822832124 2210 9 -13.278722508016397 2210 10 -7.2963493789063385 2210 11 -10.318526715157935 2210 12 -4.860751718719957 2210 13 -5.108189824710868 2210 14 -4.159083472968643 2210 15 -5.675622306234509 2210 16 -5.506748982108746 2210 17 -4.998474656448376 2210 18 -5.132509267879495 2210 19 -10.345538991771514 2210 20 -7.445105456269353 2210 21 -5.657275617033296 2210 22 -10.252323053857902 2210 23 -8.225224751366124 2210 24 -11.142662143959354 2210 25 -8.610214695021469 2211 1 -0.7115211837722022 2211 2 4.047078469121777 2211 3 -0.17279551842410257 2211 4 0.7816125366884421 2211 5 0.028958800645916938 2211 6 -0.9466450574720042 2211 7 -20.713143526945753 2211 8 -13.778377906944273 2211 9 -9.651599118827875 2211 10 -7.895959378195204 2211 11 -10.133717280284685 2211 12 -5.653016080814282 2211 13 -7.367168262387482 2211 14 -5.477175838012304 2211 15 -6.055087758234855 2211 16 -12.391058751963412 2211 17 -8.578752298029489 2211 18 -6.569673865396513 2211 19 -9.328750995708948 2211 20 -7.341273942565056 2211 21 -5.0066166954863185 2211 22 -6.533872876261608 2211 23 -8.430527563338622 2211 24 -21.903014566866844 2211 25 -21.693915135550103 2212 1 -1.2578819880769316 2212 2 -13.760058319323422 2212 3 -7.935768371116582 2212 4 -19.661887888120393 2212 5 -19.92686108040761 2212 6 -20.754631463589252 2212 7 -10.108814522616749 2212 8 -9.55492845919576 2212 9 -5.46407636121166 2212 10 -5.682389770233073 2212 11 -5.355046729651519 2212 12 -3.0015454330819544 2212 13 -6.327656994838831 2212 14 -4.126922975609908 2212 15 -4.286313230581822 2212 16 -4.242157186239417 2212 17 -4.347510642488768 2212 18 -3.4856911814138307 2212 19 -3.875508288260633 2212 20 -4.176909335209796 2212 21 -1.929827441126971 2212 22 -3.309088315028447 2212 23 -3.5296738998681363 2212 24 -2.4458717097239884 2212 25 -2.885257126892096 2213 1 -18.391215321410264 2213 2 -13.760058319323422 2213 3 -8.589348941318407 2213 4 -5.564703510282065 2213 5 -19.92686108040761 2213 6 -10.683786776940751 2213 7 -20.713143526945753 2213 8 -10.723287801942025 2213 9 -8.271417975092785 2213 10 -7.146676074301393 2213 11 -6.338502147984322 2213 12 -5.050171829392273 2213 13 -19.032418899600735 2213 14 -12.249317021164718 2213 15 -6.997246620534284 2213 16 -6.204592752489695 2213 17 -4.868472695875887 2213 18 -4.940163502916125 2213 19 -5.877605555208039 2213 20 -5.743473379702994 2213 21 -3.9317029498144684 2213 22 -4.784240773832135 2213 23 -4.4784618966125365 2213 24 -3.612369698565299 2213 25 -4.511353827648197 2214 1 -18.391215321410264 2214 2 -13.760058319323422 2214 3 -12.539999262822624 2214 4 -19.661887888120393 2214 5 -19.92686108040761 2214 6 -20.754631463589252 2214 7 -16.70817334801931 2214 8 -15.291328242315226 2214 9 -14.251197788492133 2214 10 -14.294158121166435 2214 11 -14.745350792719 2214 12 -12.731151347379717 2214 13 -19.032418899600735 2214 14 -16.86066166899075 2214 15 -23.643023187291778 2214 16 -18.3993361901381 2214 17 -23.355302850280975 2214 18 -17.879361195958595 2214 19 -15.141524313322385 2214 20 -14.02825063804215 2214 21 -11.595254790450939 2214 22 -12.52962225425026 2214 23 -12.554280373579978 2214 24 -11.481543393904618 2214 25 -11.21876603018231 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 129865.0 3 5.0 4 687.0 5 2338.0 6 3109.0 7 3066.0 8 2719.0 9 2531.0 10 2315.0 11 2158.0 12 2026.0 13 2127.0 14 3332.0 15 6972.0 16 10591.0 17 11150.0 18 11635.0 19 13265.0 20 15589.0 21 18175.0 22 18743.0 23 19960.0 24 21536.0 25 21617.0 26 18438.0 27 14374.0 28 15097.0 29 17683.0 30 16991.0 31 19444.0 32 25095.0 33 31577.0 34 38507.0 35 44733.0 36 44056.0 37 46613.0 38 34896.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.27755911436301 19.534910162219642 13.389557883754295 25.797972839663053 2 12.883466454404891 20.664415959889844 44.04957959359189 22.402537992113377 3 18.03041974459936 25.937957470054414 30.349158024926272 25.68246476041996 4 10.943869235448108 17.613829211655627 39.95389366642807 31.488407886468195 5 11.961310366670073 38.40023192330444 37.334959924524384 12.303497785501106 6 29.736370695292063 38.66574487794246 19.408136715344376 12.189747711421099 7 25.78039091763662 32.06118322249301 23.971515073167385 18.18691078670299 8 22.784576440365594 39.93410286336513 21.75857116483984 15.522749531429431 9 26.310459843670774 15.261095229083995 21.24037748986287 37.18806743738236 10 14.210790784074446 30.064268877426198 35.84128942090621 19.883650917593148 11 32.443247225675734 22.028518365028933 27.10779573605931 18.42043867323602 12 23.91390784393494 24.806607358054634 33.27090321540693 18.00858158260349 13 28.601775610972226 22.488590799232693 25.905291514601696 23.004342075193385 14 18.92214655215415 24.049828788727933 28.732097638839576 28.295927020278338 15 20.991895452076 34.55826864827453 23.60455164509671 20.845284254552762 16 19.49014528998452 28.428754317017983 31.316839347385972 20.76426104561153 17 19.582827417089256 29.63687117586839 30.788723424517293 19.991577982525058 18 19.488676924790855 27.576587788463936 34.00880398598047 18.92593130076474 19 22.56859791832716 26.872890196446154 28.08104575929486 22.47746612593183 20 22.791534262909003 28.632204066748855 29.361075899911445 19.215185770430697 21 20.966362448739858 26.66647492207853 27.533261378653677 24.83390125052793 22 21.615898976233414 30.31270588684129 28.622177745365757 19.44921739155954 23 20.061338260426133 29.613696085243713 29.89756250478528 20.427403149544876 24 22.140633653533985 27.64483827639109 30.395826569651597 19.818701500423327 25 21.10828767113393 28.3350259526943 30.150920009650093 20.405766366521675 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 735.0 1 735.0 2 1599.5 3 2464.0 4 2464.0 5 2464.0 6 3559.0 7 4654.0 8 4654.0 9 4654.0 10 5452.0 11 6250.0 12 6250.0 13 6250.0 14 7924.0 15 9598.0 16 9598.0 17 9598.0 18 14236.5 19 18875.0 20 18875.0 21 18875.0 22 26422.5 23 33970.0 24 33970.0 25 33970.0 26 44377.5 27 54785.0 28 54785.0 29 54785.0 30 65751.5 31 76718.0 32 76718.0 33 76718.0 34 81840.5 35 86963.0 36 86963.0 37 86963.0 38 87593.5 39 88224.0 40 88224.0 41 88224.0 42 86425.5 43 84627.0 44 84627.0 45 84627.0 46 80744.5 47 76862.0 48 76862.0 49 76862.0 50 68270.0 51 59678.0 52 59678.0 53 59678.0 54 49392.5 55 39107.0 56 39107.0 57 39107.0 58 31776.5 59 24446.0 60 24446.0 61 24446.0 62 19100.5 63 13755.0 64 13755.0 65 13755.0 66 10247.5 67 6740.0 68 6740.0 69 6740.0 70 4795.5 71 2851.0 72 2851.0 73 2851.0 74 1994.0 75 1137.0 76 1137.0 77 1137.0 78 762.5 79 388.0 80 388.0 81 388.0 82 261.0 83 134.0 84 134.0 85 134.0 86 88.5 87 43.0 88 43.0 89 43.0 90 25.0 91 7.0 92 7.0 93 7.0 94 4.5 95 2.0 96 2.0 97 2.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.699126281537918 2 36.91391961212961 3 5.964228768497074 4 14.122205147074737 5 13.890897022430973 6 15.259554266502168 7 9.586805480400857 8 7.768518718931047 9 0.10937714190890528 10 1.5027091765690497 11 5.98904785610701 12 6.005642013520631 13 16.653463489246263 14 1.6451303362842074 15 3.054767934315996 16 3.0670331810999762 17 3.0254756390554314 18 1.5618709551741305 19 1.513242859101174 20 0.28094629986363934 21 4.677243638305087 22 1.6611473056138755 23 0.11370605253854534 24 9.330101080063201 25 0.11457183466447335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 693015.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.97180120328694 #Duplication Level Percentage of deduplicated Percentage of total 1 96.69922933370886 87.00203838123505 2 2.2359861859199777 4.02351409225776 3 0.44905788724567947 1.2120764088010894 4 0.18363158750675773 0.6608665874320798 5 0.1055672635953151 0.474903842688634 6 0.07043060695804154 0.380206114071345 7 0.04226154359842467 0.26616430394270557 8 0.032266434011309214 0.23224553491235908 9 0.028537239362954388 0.23107921441689125 >10 0.12959337232258597 2.239997096788299 >50 0.012556513596763862 0.7731850627739212 >100 0.010044952776656312 1.7566130333659669 >500 5.02247637944047E-4 0.3812371448450639 >1k 3.348317586293647E-4 0.36587318246885037 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1256 0.18123705836093013 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1173 0.169260405618926 No Hit CTGTAGGACGTGGAATATGGCAAGA 896 0.12929013080524954 No Hit CTTTAGGACGTGAAATATGGCGAGG 837 0.12077660656695742 No Hit GTCCTACAGTGTGCATTTCTCATTT 798 0.11514902274842537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.442970209880017E-4 0.0 10 0.0 0.0 0.0 1.442970209880017E-4 0.0 11 0.0 0.0 0.0 2.885940419760034E-4 0.0 12 0.0 0.0 0.0 2.885940419760034E-4 0.0 13 0.0 0.0 0.0 2.885940419760034E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAT 50 1.09377215E-4 18.758507 1 CCAACGT 30 0.0016333584 16.990467 19 TGTAGGA 775 0.0 16.31249 2 CAAGGCT 40 0.0023115906 16.129292 4 CCTAGGG 70 6.5729815E-5 15.704572 2 GTCCTAT 60 4.4027992E-4 15.632089 1 CTGTAGG 770 0.0 15.487079 1 GCTGACT 45 5.4567016E-4 15.198515 8 TAGGACC 340 0.0 15.18051 4 ATAAGAC 45 0.004374813 14.656054 3 GTTGTGA 55 0.0044511114 14.617018 1 GATATAC 175 0.0 14.547413 1 GTACTAA 65 8.081854E-4 14.429622 1 GCCTAGG 65 8.081854E-4 14.429622 1 TAATTCC 45 0.0050631454 14.337148 4 TTTAGGA 560 0.0 14.232268 2 TCTATAG 85 7.624367E-7 14.224994 3 GTATTAT 85 2.6674714E-5 14.187106 1 GTCCTAG 95 4.854741E-6 14.10414 1 CTTTAGG 565 0.0 13.991807 1 >>END_MODULE