FastQCFastQC Report
Thu 26 May 2016
SRR1926666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926666_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693015
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGGACGTGAAATATGGCGAGG26350.3802226503033845No Hit
CTGTAGGACGTGGAATATGGCAAGA25850.3730077992539844No Hit
GTCCTAAAGTGTGTATTTCTCATTT25010.36088684949099226No Hit
GTCCTACAGTGGACATTTCTAAATT24320.3509303550428201No Hit
GTCCTACAGTGTGCATTTCTCATTT17280.24934525226726698No Hit
CTGTAGGACCTGGAATATGGCGAGA14780.21327099702026653No Hit
CTGAAGGACCTGGAATATGGCGAGA13360.19278082003997027No Hit
GTATCAACGCAGAGTACTTTTTTTT11480.16565298009422594No Hit
CCCATGTACTCTGCGTTGATACCAC9650.13924662525342163No Hit
GTACATGGGAAGCAGTGGTATCAAC8800.1269813784694415No Hit
GAATATGGCAAGAAAACTGAAAATC8440.12178668571387342No Hit
GTCCTTCAGTGTGCATTTCTCATTT7900.11399464658052134No Hit
GGTATCAACGCAGAGTACTTTTTTT7650.11038722105582131No Hit
GGAATATGGCGAGAAAACTGAAAAT7530.10865565680396529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAA350.002210898316.23864618
GACTAGG456.583471E-414.8281561
GTTTAGC400.00515737514.2985811
GTCCTAG1051.2969394E-913.6176961
GGACTTG1301.2732926E-1113.2063616
ATAGGAC1800.012.7190853
TAGGACC5300.012.4182044
ACAGGTC701.0863085E-412.2177758
CCGGTTT701.1092836E-412.19041912
TTATCAG550.002973705512.1400635
GGCTTAG550.002988307712.1321291
GAGGGTA550.003058926212.0943649
CTATAGG1459.094947E-1111.8333071
GACGTGG6200.011.8009047
TGTAGGA14600.011.695462
TAGGACT1655.456968E-1211.5619644
GAAGTGT1001.8341871E-611.4455136
GTATAGG752.0061861E-411.4388651
CTGTAGG14850.011.4260251
GTAGGAC15000.011.2563913
Produced by FastQC (version 0.11.5)