FastQCFastQC Report
Thu 26 May 2016
SRR1926665_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926665_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36142
Sequences flagged as poor quality0
Sequence length25
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC4501.2450888163355651No Hit
CCCATGTACTCTGCGTTGATACCAC4271.1814509435006364No Hit
CATGTACTCTGCGTTGATACCACTG1740.4814343423164186No Hit
GAGTACATGGGAAGCAGTGGTATCA1580.43716451773559845No Hit
GCGTTGATACCACTGCTTCCCATGT1500.4150296054451884No Hit
ACGCAGAGTACATGGGAAGCAGTGG1280.3541585966465608No Hit
CNCATGTACTCTGCGTTGATACCAC1220.33755741242875326No Hit
GTATCAACGCAGAGTACATGGGAAG1150.31818936417464444No Hit
ACTCTGCGTTGATACCACTGCTTCC1040.28775385977533063No Hit
GGTATCAACGCAGAGTACATGGGAA970.2683858115212219No Hit
GNACATGGGAAGCAGTGGTATCAAC950.2628520834486193No Hit
TATCAACGCAGAGTACATGGGAAGC910.25178462730341433No Hit
GTATCAACGCAGAGTACTTTTTTTT910.25178462730341433No Hit
GGTATCAACGCAGAGTACTTTTTTT900.24901776326711306No Hit
TATCAACGCAGAGTACTTTTTTTTT770.21304853079519676No Hit
GCTTCCCATGTACTCTGCGTTGATA650.17984616235958165No Hit
CAGTGGTATCAACGCAGAGTACATG640.1770792983232804No Hit
GTGGTATCAACGCAGAGTACATGGG580.16047811410547286No Hit
GTACTCTGCGTTGATACCACTGCTT580.16047811410547286No Hit
GGGAAGCAGTGGTATCAACGCAGAG500.13834320181506282No Hit
GNGTACATGGGAAGCAGTGGTATCA470.13004260970615905No Hit
CATGGGAAGCAGTGGTATCAACGCA470.13004260970615905No Hit
GCAGAGTACATGGGAAGCAGTGGTA470.13004260970615905No Hit
CNTGTACTCTGCGTTGATACCACTG460.12727574566985778No Hit
ACGCAGAGTACTTTTTTTTTTTTTT460.12727574566985778No Hit
ATACCACTGCTTCCCATGTACTCTG450.12450888163355653No Hit
CTGCTTCCCATGTACTCTGCGTTGA450.12450888163355653No Hit
GTACTGGTTCACTATCGGTCAGTCA440.12174201759725528No Hit
GNGTTGATACCACTGCTTCCCATGT420.11620828952465276No Hit
CCACTGCTTCCCATGTACTCTGCGT420.11620828952465276No Hit
GTANATGGGAAGCAGTGGTATCAAC420.11620828952465276No Hit
GATACCACTGCTTCCCATGTACTCT410.1134414254883515No Hit
TACCACTGCTTCCCATGTACTCTGC400.11067456145205025No Hit
CCCCTGTACTCTGCGTTGATACCAC390.10790769741574899No Hit
CCATGTACTCTGCGTTGATACCACT390.10790769741574899No Hit
CTTCCCATGTACTCTGCGTTGATAC380.10514083337944773No Hit
GCAGTGGTATCAACGCAGAGTACAT370.10237396934314649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT2151.2840246E-88.7613796
CCATGTA2101.1522216E-58.6489792
GAAGCAG2202.1609594E-98.3732889
ATGTACT2201.0298754E-78.3631794
GGAAGCA2352.4592737E-98.3082988
TGTACTC2355.8438673E-88.0157315
TACATGG3853.601599E-107.9836742
TACTCTG2202.6004273E-77.9020917
TGGGAAG2603.830246E-87.6474866
AAGCAGT2554.100002E-97.58521310
GGGAAGC2451.7538878E-77.5131537
ATGGGAA2801.731496E-87.4749865
AGTGGTA3001.8189894E-127.33976614
CATGGGA3107.514245E-97.3316674
TGGTATC3007.2759576E-127.323903616
CATGTAC2453.2990683E-67.24292043
GTACATG4152.8394425E-97.2190331
CAGTGGT2651.2072633E-87.176100713
GTGGTAT2953.8198777E-117.172183515
AGCAGTG2751.7682396E-87.033561711
Produced by FastQC (version 0.11.5)