FastQCFastQC Report
Thu 26 May 2016
SRR1926665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926665_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36142
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC13443.7186652647888883No Hit
GTACATGGGAAGCAGTGGTATCAAC11693.2344640584361684No Hit
GAGTACATGGGAAGCAGTGGTATCA5371.4858059874937746No Hit
GTATCAACGCAGAGTACTTTTTTTT4811.3308616014609043No Hit
CATGTACTCTGCGTTGATACCACTG4451.231254496154059No Hit
ACGCAGAGTACATGGGAAGCAGTGG3941.0901444303026948No Hit
GGTATCAACGCAGAGTACTTTTTTT3480.9628686846328371No Hit
GCGTTGATACCACTGCTTCCCATGT3330.9213657240883183No Hit
GTATCAACGCAGAGTACATGGGAAG3020.8355929389629794No Hit
ACTCTGCGTTGATACCACTGCTTCC2840.7857893863095566No Hit
GGTATCAACGCAGAGTACATGGGAA2570.7110840573294228No Hit
TATCAACGCAGAGTACATGGGAAGC2470.6834154169664103No Hit
TATCAACGCAGAGTACTTTTTTTTT2270.6280781362403852No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1930.5340047590061425No Hit
GCTTCCCATGTACTCTGCGTTGATA1570.4343976536992972No Hit
GTGGTATCAACGCAGAGTACATGGG1560.43163078966299595No Hit
CTGCTTCCCATGTACTCTGCGTTGA1560.43163078966299595No Hit
ATACCACTGCTTCCCATGTACTCTG1490.4122627414088872No Hit
CATGGGAAGCAGTGGTATCAACGCA1480.40949587737258597No Hit
GTACTCTGCGTTGATACCACTGCTT1460.40396214929998336No Hit
CAGTGGTATCAACGCAGAGTACATG1450.40119528526368214No Hit
GGGAAGCAGTGGTATCAACGCAGAG1350.37352664490066956No Hit
GCAGAGTACATGGGAAGCAGTGGTA1310.36245918875546457No Hit
GATACCACTGCTTCCCATGTACTCT1210.334790548392452No Hit
AAGCAGTGGTATCAACGCAGAGTAC1160.3209562282109457No Hit
GCAGTGGTATCAACGCAGAGTACAT1100.3043550439931382No Hit
CCACTGCTTCCCATGTACTCTGCGT1080.2988213159205357No Hit
TACCACTGCTTCCCATGTACTCTGC1080.2988213159205357No Hit
CTTCCCATGTACTCTGCGTTGATAC1030.2849869957390294No Hit
ACATGGGAAGCAGTGGTATCAACGC910.25178462730341433No Hit
GTTGATACCACTGCTTCCCATGTAC890.2462508992308118No Hit
CCATGTACTCTGCGTTGATACCACT860.23795030712190804No Hit
GTACTGGTTCACTATCGGTCAGTCA770.21304853079519676No Hit
ATGGGAAGCAGTGGTATCAACGCAG760.21028166675889545No Hit
GAGTACTTTTTTTTTTTTTTTTTTT670.18537989043218417No Hit
GCAGAGTACTTTTTTTTTTTTTTTT640.1770792983232804No Hit
ATCAACGCAGAGTACATGGGAAGCA640.1770792983232804No Hit
GTACTTTTTTTTTTTTTTTTTTTTT600.16601184217807538No Hit
AAAAAGTACTCTGCGTTGATACCAC550.1521775219965691No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA540.14941065796026784No Hit
ATCAACGCAGAGTACTTTTTTTTTT540.14941065796026784No Hit
CCCATATTCAGACAGGATACCACGT490.13557633777876155No Hit
GGATACCACGTGTCCCGCCCTACTC480.1328094737424603No Hit
GTACATGGTAAGCAGTGGTATCAAC450.12450888163355653No Hit
GTGGTATCAACGCAGAGTACTTTTT420.11620828952465276No Hit
GGAAGCAGTGGTATCAACGCAGAGT410.1134414254883515No Hit
CCCCATGTACTCTGCGTTGATACCA410.1134414254883515No Hit
CTATCGGTCAGTCAGGAGTATTTAG380.10514083337944773No Hit
TTCCCATGTACTCTGCGTTGATACC380.10514083337944773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCAG3650.08.8467319
GGAAGCA3650.08.8467318
AGCAGTG4000.08.77276711
CAGTGGT3700.08.71509313
AGTGGTA3650.08.5746414
GGGAAGC3800.08.509327
AAGCAGT4150.08.45567910
GCAGTGG4250.08.256721512
TGGGAAG4200.07.936376
GTGGTAT4000.07.8243615
TGGTATC4050.07.727762716
CATGGGA5150.07.21207954
ATGGGAA4900.07.19132335
CCATGTA3006.7338988E-96.99373252
TGCGTTG3152.6757334E-96.92487812
CTGCGTT3203.7180143E-96.81667711
CTCTGCG3351.2751116E-96.80398279
TCTGCGT3255.136826E-96.71180510
TTGATAC3255.136826E-96.71180516
TGATACC3452.4883775E-96.597615217
Produced by FastQC (version 0.11.5)