FastQCFastQC Report
Thu 26 May 2016
SRR1926664_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926664_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40405
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC5271.3042940230169533No Hit
GTACATGGGAAGCAGTGGTATCAAC4451.1013488429649796No Hit
GAGTACATGGGAAGCAGTGGTATCA2550.6311100111372355No Hit
CATGTACTCTGCGTTGATACCACTG1880.4652889493874521No Hit
ACGCAGAGTACATGGGAAGCAGTGG1530.3786660066823413No Hit
GCGTTGATACCACTGCTTCCCATGT1520.3761910654621953No Hit
GTATCAACGCAGAGTACTTTTTTTT1350.33411706471971286No Hit
ACTCTGCGTTGATACCACTGCTTCC1280.3167924761786908No Hit
GGTATCAACGCAGAGTACATGGGAA1260.3118425937383987No Hit
GTATCAACGCAGAGTACATGGGAAG1220.30194282885781465No Hit
CNCATGTACTCTGCGTTGATACCAC1120.2771934166563544No Hit
TATCAACGCAGAGTACATGGGAAGC1100.27224353421606234No Hit
GNACATGGGAAGCAGTGGTATCAAC1020.2524440044548942No Hit
GGTATCAACGCAGAGTACTTTTTTT940.23264447469372604No Hit
GCTTCCCATGTACTCTGCGTTGATA800.19799529761168172No Hit
TATCAACGCAGAGTACTTTTTTTTT730.18067070907065957No Hit
GTACTCTGCGTTGATACCACTGCTT700.1732458854102215No Hit
CTGCTTCCCATGTACTCTGCGTTGA680.16829600296992947No Hit
CAGTGGTATCAACGCAGAGTACATG620.15344635564905335No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA610.15097141442890732No Hit
ATACCACTGCTTCCCATGTACTCTG600.14849647320876128No Hit
GTGGTATCAACGCAGAGTACATGGG550.13612176710803117No Hit
CCACTGCTTCCCATGTACTCTGCGT550.13612176710803117No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA540.13364682588788518No Hit
GATACCACTGCTTCCCATGTACTCT540.13364682588788518No Hit
CATGGGAAGCAGTGGTATCAACGCA520.1286969434475931No Hit
TACCACTGCTTCCCATGTACTCTGC520.1286969434475931No Hit
GGGAAGCAGTGGTATCAACGCAGAG500.12374706100730108No Hit
GCAGAGTACATGGGAAGCAGTGGTA500.12374706100730108No Hit
GNGTACATGGGAAGCAGTGGTATCA470.11632223734686302No Hit
CNTGTACTCTGCGTTGATACCACTG460.113847296126717No Hit
CCATGTACTCTGCGTTGATACCACT460.113847296126717No Hit
AAGCAGTGGTATCAACGCAGAGTAC440.10889741368642494No Hit
GTACTGGTTCACTATCGGTCAGTCA430.10642247246627894No Hit
GTTGATACCACTGCTTCCCATGTAC410.10147259002598688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACACA300.00958745617.4623186
CCATGTA2057.1468094E-910.7845152
ATGTACT2501.8189894E-129.8973934
TGTACTC2650.09.8843315
CCCATGT1956.7992005E-79.6762461
CATGTAC2651.8189894E-129.559073
GTACTCT2850.09.5583226
TACTCTG2651.8189894E-129.2634147
TGCGTTG2650.08.90185312
TTGATAC2750.08.89334516
GATACCA2750.08.88546818
TGATACC2750.08.75124117
TCTGCGT2701.8189894E-128.56872610
CTGCGTT2607.2759576E-128.54236111
ATACCAC2900.08.42587419
GCGTTGA2800.08.42496813
ACTCTGC2801.0913936E-118.4101928
CTCTGCG2651.2732926E-118.3811849
GTTGATA2950.08.29040615
CGTTGAT2950.07.998185614