##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926664_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40405 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.852097512684075 26.0 2.0 30.0 2.0 31.0 2 15.313352307882688 10.0 2.0 30.0 2.0 31.0 3 18.817522583838635 16.0 7.0 30.0 2.0 31.0 4 20.871476302437816 27.0 2.0 35.0 2.0 37.0 5 21.34733325083529 28.0 2.0 35.0 2.0 37.0 6 21.976710803118426 30.0 2.0 35.0 2.0 37.0 7 21.889023635688652 30.0 2.0 35.0 2.0 37.0 8 22.009083034277936 30.0 2.0 35.0 2.0 37.0 9 24.298774904096028 31.0 2.0 37.0 2.0 39.0 10 24.93884420245019 31.0 2.0 37.0 2.0 39.0 11 22.117039970300706 28.0 2.0 37.0 2.0 39.0 12 23.231134760549438 30.0 2.0 37.0 2.0 39.0 13 20.020814255661428 25.0 2.0 35.0 2.0 39.0 14 23.20086622942705 29.0 2.0 37.0 2.0 40.0 15 24.197698304665263 30.0 2.0 38.0 2.0 40.0 16 24.500928102957555 31.0 2.0 38.0 2.0 40.0 17 23.90882316544982 30.0 2.0 38.0 2.0 40.0 18 24.041133523078827 30.0 2.0 38.0 2.0 40.0 19 24.315975745576043 30.0 2.0 38.0 2.0 40.0 20 24.559509961638412 31.0 2.0 38.0 2.0 40.0 21 22.507709441900754 30.0 2.0 37.0 2.0 40.0 22 23.409701769582973 30.0 2.0 37.0 2.0 40.0 23 23.392451429278555 30.0 2.0 38.0 2.0 40.0 24 22.492092562801634 30.0 2.0 38.0 2.0 40.0 25 22.322781833931444 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.57382816741125 1101 2 12.001535919453914 1101 3 8.806246606294785 1101 4 10.033430743620798 1101 5 9.15960168456482 1101 6 9.84708279664158 1101 7 9.834989098486158 1101 8 7.9294346396165984 1101 9 6.76355487437786 1101 10 6.576058256350958 1101 11 9.099380309223935 1101 12 7.328607238311186 1101 13 11.82815425745192 1101 14 8.55890643843734 1101 15 8.229956596047902 1101 16 7.7886020837130445 1101 17 2.7969222963733067 1101 18 6.4776943554867366 1101 19 6.009460777348817 1101 20 5.150775130047705 1101 21 3.519089982276963 1101 22 6.152598191264893 1101 23 7.090457320632652 1101 24 7.873507734710607 1101 25 8.55002445736076 1102 1 6.890692462536414 1102 2 12.32432906833402 1102 3 8.721266369140636 1102 4 9.78639517049827 1102 5 9.900049642404081 1102 6 8.420864087814174 1102 7 9.114962748025025 1102 8 9.594783783226614 1102 9 8.437467917856122 1102 10 7.728232169394435 1102 11 9.527575302636322 1102 12 6.817408292329631 1102 13 11.257666773920956 1102 14 9.392239771770672 1102 15 8.697677281159894 1102 16 8.390051359075365 1102 17 8.735657474238923 1102 18 8.83474310383983 1102 19 6.774282911736169 1102 20 8.496624932419245 1102 21 7.421593276084604 1102 22 8.549831392845924 1102 23 8.861208308774945 1102 24 10.626472161588076 1102 25 11.02103895011438 1103 1 4.959621186394717 1103 2 8.364875376485962 1103 3 5.694591166409097 1103 4 7.712344807715514 1103 5 5.86974661210105 1103 6 6.421077489436026 1103 7 6.947869278114652 1103 8 6.686119677379409 1103 9 6.219158058701193 1103 10 5.270485690521195 1103 11 6.068761815653822 1103 12 3.922401890280973 1103 13 8.510334294194113 1103 14 5.96266230698194 1103 15 5.6584113827390645 1103 16 5.415872955319493 1103 17 2.848973735442506 1103 18 1.0263777602632231 1103 19 3.8641249945359952 1103 20 5.133628773648439 1103 21 7.48091892695955 1103 22 3.2679278503789995 1103 23 4.941447318591418 1103 24 6.79655325420438 1103 25 2.8121187623209565 1104 1 6.165002729383996 1104 2 10.720982166479654 1104 3 6.372329236621415 1104 4 7.22443225778909 1104 5 7.436910791205527 1104 6 6.985088420695224 1104 7 5.8944742812679 1104 8 6.023096763778398 1104 9 5.2359753805426905 1104 10 4.078978438051152 1104 11 6.818393936738538 1104 12 4.057571114582004 1104 13 8.224650039411685 1104 14 6.280299473263209 1104 15 5.671444807166225 1104 16 5.855225488428598 1104 17 6.696987189299978 1104 18 3.314164180275835 1104 19 4.178398696448966 1104 20 2.2734227614558797 1104 21 1.9326742349267576 1104 22 3.9259055674275594 1104 23 4.860303740706197 1104 24 5.313265467784369 1104 25 4.943924522253688 1105 1 0.6462919355271914 1105 2 7.58124606438145 1105 3 4.3714639975991325 1105 4 2.294300308838192 1105 5 -0.24619541688445779 1105 6 2.0499813473662165 1105 7 3.139336924573115 1105 8 0.7265360888919616 1105 9 1.5026853091604693 1105 10 1.416637966495884 1105 11 4.128365816470314 1105 12 2.1836797020792993 1105 13 2.4803281704953974 1105 14 1.4429644094518324 1105 15 2.5343044221348627 1105 16 2.1509209242927554 1105 17 1.8114150499964978 1105 18 -0.3194070937886231 1105 19 0.44424338604446945 1105 20 -1.0086451598073687 1105 21 -1.1910549452592711 1105 22 0.3410039883663458 1105 23 -1.9240354329905394 1105 24 1.931478749203361 1105 25 1.6514737327230797 1106 1 5.621593017380633 1106 2 10.139523263809902 1106 3 7.8776897579583895 1106 4 8.317978610532414 1106 5 7.573971964213726 1106 6 6.378823968309266 1106 7 6.074629841494936 1106 8 5.503021085830113 1106 9 5.021974960109642 1106 10 4.3972462539014785 1106 11 6.674395618470722 1106 12 4.246345552252805 1106 13 2.158221618137773 1106 14 3.9063242788129244 1106 15 4.573904340485544 1106 16 5.120098307432169 1106 17 5.651790636850958 1106 18 6.012293253220971 1106 19 6.216237670592335 1106 20 5.8096851116766075 1106 21 5.945707659353918 1106 22 5.324265878548015 1106 23 6.5189121073238105 1106 24 7.134581423218464 1106 25 3.9811328468279967 1107 1 3.418128989972942 1107 2 -13.099913355908406 1107 3 -5.292656644782378 1107 4 0.4456908297939286 1107 5 4.518395260749699 1107 6 4.129299796249885 1107 7 4.355748988811264 1107 8 4.670131858574688 1107 9 3.4104408908290935 1107 10 2.147151088313354 1107 11 4.769180544241564 1107 12 1.259668734590072 1107 13 3.072572588826773 1107 14 1.9462938258247249 1107 15 2.156317739800354 1107 16 2.0702315392555413 1107 17 2.2258294644109107 1107 18 -1.7467474776507466 1107 19 0.2149053523586062 1107 20 0.10964704544135984 1107 21 -4.4035500490587225 1107 22 -0.5234691804546507 1107 23 -2.5372355896689562 1107 24 0.5605425956585108 1107 25 0.7868047158801481 1108 1 5.32503589687985 1108 2 10.733419977424926 1108 3 -0.28252150964724265 1108 4 5.134880018983118 1108 5 6.3975243898788285 1108 6 7.4461166130667 1108 7 7.439457697519973 1108 8 6.9723090357518664 1108 9 5.9652456956339 1108 10 5.506009947172213 1108 11 7.887423787484806 1108 12 3.991650716572053 1108 13 4.531656672910856 1108 14 4.93390643843734 1108 15 5.037323745806358 1108 16 4.848384692408697 1108 17 -0.8135849500035022 1108 18 3.8031774472741766 1108 19 5.433373820827075 1108 20 5.542079477873791 1108 21 4.886239740731067 1108 22 3.0662455342600623 1108 23 5.6339355815022145 1108 24 6.183290343406259 1108 25 6.798816727892159 1109 1 4.512337484181437 1109 2 -13.099913355908406 1109 3 -4.686092938218671 1109 4 3.763451447554548 1109 5 3.86974661210105 1109 6 3.338180105130192 1109 7 4.356521189583468 1109 8 4.420324908767739 1109 9 4.5517536321418355 1109 10 4.199660740823006 1109 11 3.3981380731990924 1109 12 3.9940316689530064 1109 13 4.2026884189426035 1109 14 5.292239771770671 1109 15 4.654387237869852 1109 16 4.7281465971706 1109 17 4.901891240472686 1109 18 5.084526653623385 1109 19 3.092897630350887 1109 20 2.4277937635880775 1109 21 1.164017518508846 1109 22 4.146801089815618 1109 23 3.8006022481688824 1109 24 5.17019510531102 1109 25 5.8543722834477165 1110 1 3.5055914524354037 1110 2 9.177864421869373 1110 3 5.995256268130536 1110 4 6.370991130094229 1110 5 6.550302167656607 1110 6 3.1822277241778103 1110 7 4.425568808631084 1110 8 5.5000868135296415 1110 9 4.020801251189454 1110 10 3.311565502727767 1110 11 1.3179793430403635 1110 12 3.5610951610164996 1110 13 7.7677677840219665 1110 14 6.7811286606595615 1110 15 5.676212634695247 1110 16 4.987273581297586 1110 17 5.2975261611076085 1110 18 5.053177447274177 1110 19 4.183373820827075 1110 20 4.153190588984902 1110 21 2.122350851842178 1110 22 4.10791220092673 1110 23 4.564491137057772 1110 24 6.586068121184034 1110 25 6.076594505669938 1111 1 6.526237098081051 1111 2 11.035221779226731 1111 3 9.20734335521762 1111 4 9.742988127091227 1111 5 9.896773639128078 1111 6 8.440110607060696 1111 7 8.86926250232478 1111 8 8.480942669385499 1111 9 7.478008458396662 1111 10 6.944448385610652 1111 11 8.796207571268589 1111 12 8.124533599454939 1111 13 9.545545561799745 1111 14 7.730077609608511 1111 15 8.021182604665217 1111 16 7.6513126203366255 1111 17 8.874478113059556 1111 18 8.037036306133036 1111 19 6.75544589289915 1111 20 6.636674072468384 1111 21 9.93428778877912 1111 22 9.679233522248055 1111 23 9.706007653574286 1111 24 8.803785838901753 1111 25 8.44896687804231 1112 1 -2.287662515818564 1112 2 5.928658072663023 1112 3 4.513907061781328 1112 4 -0.9936914095883118 1112 5 1.5697466121010493 1112 6 0.9524658194159059 1112 7 3.8136640467263234 1112 8 4.420324908767739 1112 9 2.1088964892846924 1112 10 1.31394645510872 1112 11 -1.130433355372336 1112 12 2.479745954667294 1112 13 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4.581033865136963 2204 5 -1.1148687725143347 2204 6 2.1469713139214015 2204 7 3.3730047060669825 2204 8 3.86098424942708 2204 9 3.0297756101638136 2204 10 2.3666937078559727 2204 11 3.2585776336386516 2204 12 4.169855844777182 2204 13 2.3070840233382057 2204 14 4.246085925616825 2204 15 3.693947677430291 2204 16 3.9413334103574122 2204 17 0.5447483833298286 2204 18 2.755955225051956 2204 19 4.111578949032204 2204 20 4.188233324027639 2204 21 5.664017518508846 2204 22 5.485262628277159 2204 23 5.046756094322728 2204 24 5.995469830585744 2204 25 5.915910744986178 2205 1 7.330086401930355 2205 2 11.081904825909778 2205 3 9.02429667217094 2205 4 8.937910322013423 2205 5 9.097019339373777 2205 6 8.572379239329326 2205 7 9.22102335408563 2205 8 8.276601965044794 2205 9 7.5283770087652115 2205 10 6.955504896667161 2205 11 8.027575302636322 2205 12 7.984074958996295 2205 13 11.74251525876944 2205 14 8.452845832376735 2205 15 6.849192432675046 2205 16 7.223384692408697 2205 17 8.068990807572256 2205 18 7.471106740203471 2205 19 5.259131396584653 2205 20 6.087534023328338 2205 21 -0.7753764208850917 2205 22 4.640740483755014 2205 23 5.982420429987066 2205 24 7.838593373709287 2205 25 7.566493495568928 2206 1 3.677931045549645 2206 2 8.590227489162018 2206 3 5.793182715704869 2206 4 -0.0482185725661779 2206 5 4.602140978298234 2206 6 5.71685213732335 2206 7 5.990122799241412 2206 8 5.9396408041399695 2206 9 4.0783129882786575 2206 10 4.298654704605703 2206 11 3.22369139311861 2206 12 3.992824425492241 2206 13 7.228644153349041 2206 14 0.8781552647284201 2206 15 1.8696789883728684 2206 16 2.951084222925129 2206 17 4.356015988963634 2206 18 -1.6355940707226928 2206 19 -0.5302412026471011 2206 20 1.1195442666061872 2206 21 1.6499330114665938 2206 22 -3.0560158115928324 2206 23 -3.2275667659156255 2206 24 -0.3020382950913927 2206 25 0.6008511566871526 2207 1 1.7139247857687359 2207 2 -13.099913355908406 2207 3 -5.949188176313909 2207 4 -3.6012310921279926 2207 5 0.61974661210105 2207 6 1.293338835288921 2207 7 2.1339021419644197 2207 8 2.861197924640752 2207 9 0.6458012511894538 2207 10 0.4504543916166561 2207 11 -0.19590954584852582 2207 12 -3.8555715056501683 2207 13 2.3233233395775237 2207 14 -4.524426894895996 2207 15 -0.30989847641586366 2207 16 0.306718025742029 2207 17 1.1725261611076085 2207 18 1.5392885583852873 2207 19 0.9889293763826323 2207 20 1.1809683667626807 2207 21 -3.9332047037133755 2207 22 -3.76708779907327 2207 23 -0.8521755296088962 2207 24 0.48884589896181296 2207 25 0.2710389501143844 2208 1 3.282813674657625 2208 2 4.086753310758262 2208 3 5.31581182368609 2208 4 6.668213352316453 2208 5 2.9630799454343837 2208 6 4.930561057511145 2208 7 5.675568808631084 2208 8 5.731753480196311 2208 9 4.630801251189453 2208 10 4.1748988360611 2208 11 4.719090454151473 2208 12 5.001650716572055 2208 13 8.352212228466414 2208 14 3.652239771770674 2208 15 4.340101523584135 2208 16 4.503384692408694 2208 17 5.278081716663163 2208 18 1.209288558385289 2208 19 3.703373820827075 2208 20 4.608746144540458 2208 21 7.250684185175512 2208 22 6.306801089815618 2208 23 0.9472689148355506 2208 24 1.121623676739592 2208 25 3.054372283447716 2209 1 2.1500773562496676 2209 2 3.6762060470766684 2209 3 3.745463564979623 2209 4 -1.4472095332556876 2209 5 3.213030194190601 2209 6 3.7761331968146266 2209 7 3.804922042461932 2209 8 3.0977236294500408 2209 9 2.5941843357665704 2209 10 1.511814258946675 2209 11 -0.2860836752017626 2209 12 0.773989025029767 2209 13 -0.23803652775249518 2209 14 1.862389025502015 2209 15 2.4624895832856275 2209 16 2.07910608544352 2209 17 -0.6910725121925587 2209 18 2.000731344454941 2209 19 2.327652427792252 2209 20 2.19879589578424 2209 21 4.61924139910586 2209 22 3.7468010898156194 2209 23 -0.13969625929380314 2209 24 -3.0001673680365286 2209 25 -0.08592622401496897 2210 1 -1.246144658675707 2210 2 -13.099913355908406 2210 3 -6.454396509647243 2210 4 -0.7713699810168819 2210 5 0.93224661210105 2210 6 -0.18235560915552185 2210 7 -0.3921395247022481 2210 8 -19.70824651980369 2210 9 -11.562532082143878 2210 10 -5.974892830605565 2210 11 -9.784451212515194 2210 12 -3.107307616761279 2210 13 -5.626329438200255 2210 14 -2.4202602282293277 2210 15 -3.7317734764158637 2210 16 -3.271406974257971 2210 17 -3.29275161667017 2210 18 -4.184669774948045 2210 19 -7.946834512506257 2210 20 -5.817295522126209 2210 21 -3.648482481491154 2210 22 -7.175073910184381 2210 23 -5.277522751831118 2210 24 -7.7958763232604085 2210 25 -5.3800027165522835 2211 1 -0.418019658675707 2211 2 4.493836644091594 2211 3 -0.25127150964724265 2211 4 0.21300501898311808 2211 5 -0.22400338789895002 2211 6 -0.38548060915552185 2211 7 -19.657764524702248 2211 8 -13.14574651980369 2211 9 -9.453157082143878 2211 10 -7.318642830605565 2211 11 -7.643826212515194 2211 12 -4.388557616761279 2211 13 -6.985704438200255 2211 14 -5.154635228229328 2211 15 -4.934898476415864 2211 16 -10.896406974257971 2211 17 -8.44900161667017 2211 18 -6.231544774948045 2211 19 -8.821834512506257 2211 20 -6.942295522126209 2211 21 -5.445357481491154 2211 22 -6.471948910184381 2211 23 -9.246272751831118 2211 24 -20.35837632326041 2211 25 -20.145627716552283 2212 1 -2.259931423381591 2212 2 -13.099913355908406 2212 3 -7.995756803764889 2212 4 -18.865119981016882 2212 5 -19.13025338789895 2212 6 -19.776105609155522 2212 7 -10.113646877643424 2212 8 -9.046481813921337 2212 9 -5.650767376261527 2212 10 -6.036473712958507 2212 11 -6.458164447809311 2212 12 -3.9299179108789275 2212 13 -5.925042673494373 2212 14 -4.563642581170505 2212 15 -5.604016123474686 2212 16 -5.075634915434442 2212 17 -4.958192793140757 2212 18 -4.941103598477458 2212 19 -5.002900688976844 2212 20 -5.281449933890915 2212 21 -3.7918648344323316 2212 22 -5.076728321949087 2212 23 -4.537633045948766 2212 24 -3.946611617378057 2212 25 -3.7779806577287545 2213 1 -17.730519658675707 2213 2 -13.099913355908406 2213 3 -8.947844090292405 2213 4 -5.945765142307204 2213 5 -19.13025338789895 2213 6 -10.6954604478652 2213 7 -19.657764524702248 2213 8 -10.724375552061755 2213 9 -8.272209501498716 2213 10 -8.529832346734597 2213 11 -7.568221373805516 2213 12 -5.688456810309667 2213 13 -17.954454438200255 2213 14 -12.027115066939006 2213 15 -7.7695758957707035 2213 16 -7.233604554903131 2213 17 -5.629445165057266 2213 18 -5.689206065270625 2213 19 -6.504798222183677 2213 20 -6.699856005997177 2213 21 -4.52953086858793 2213 22 -5.704811813410188 2213 23 -5.183268719573054 2213 24 -4.82611825874428 2213 25 -5.0327244907458315 2214 1 -17.730519658675707 2214 2 -13.099913355908406 2214 3 -12.780328527191102 2214 4 -18.865119981016882 2214 5 -19.13025338789895 2214 6 -19.776105609155522 2214 7 -16.763027682596984 2214 8 -15.164386870680882 2214 9 -14.466040854073704 2214 10 -14.455978356921355 2214 11 -15.496348142339755 2214 12 -13.389928230796366 2214 13 -17.954454438200255 2214 14 -16.61653215805389 2214 15 -22.059898476415864 2214 16 -17.93451004443341 2214 17 -21.85525161667017 2214 18 -17.552816704772606 2214 19 -15.062240214260642 2214 20 -14.159674908091121 2214 21 -12.00264914815782 2214 22 -13.025128734745785 2214 23 -12.918695997445152 2214 24 -12.060130709225321 2214 25 -11.707031225324213 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 9219.0 3 0.0 4 44.0 5 193.0 6 320.0 7 311.0 8 234.0 9 182.0 10 161.0 11 129.0 12 132.0 13 157.0 14 219.0 15 423.0 16 566.0 17 632.0 18 678.0 19 820.0 20 865.0 21 1020.0 22 1047.0 23 1097.0 24 1127.0 25 1130.0 26 991.0 27 770.0 28 805.0 29 1003.0 30 904.0 31 1098.0 32 1401.0 33 1735.0 34 2101.0 35 2409.0 36 2414.0 37 2420.0 38 1648.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.509680584009004 17.62991603427879 11.186773234844331 22.673630146867875 2 15.687135670366526 21.831829433841733 34.80396071228939 27.677074183502352 3 21.903754627181385 30.62665256478054 24.947117927022738 22.522474881015338 4 12.83181989613856 19.39423830108213 30.676840059183615 37.097101743595694 5 12.720071694949553 40.7822844092394 31.233557861871585 15.264086033939464 6 31.98620445702158 31.880084895649098 18.11107180756986 18.022638839759463 7 24.978041282389107 36.10836627140975 19.79578392621871 19.117808519982432 8 28.984610417563495 32.59524321997417 19.36881188118812 19.051334481274214 9 28.344895936570865 17.76759167492567 17.410802775024777 36.47670961347869 10 18.71193436351739 31.949967282428148 26.420697639301356 22.917400714753107 11 29.118176047397377 28.68176047397376 18.377063055438004 23.82300042319086 12 24.86876292486346 32.32674054827934 24.041571663396788 18.762924863460416 13 29.315158975590716 24.376257243222145 21.527006335004653 24.781577446182485 14 22.66024752350465 29.092833917273715 22.34265117334207 25.904267385879564 15 31.485360993306422 26.076848398140108 20.474170967247456 21.963619641306014 16 19.674854937245982 30.075918304746814 27.379156974514967 22.87006978349224 17 23.887156947922524 30.6102110696581 24.39055552716308 21.112076455256297 18 24.2275440054393 25.98776157739669 27.279595074412633 22.50509934275138 19 23.28618884638615 31.22357559895309 26.91513992349507 18.575095631165695 20 25.311491685281705 26.952097294614045 25.264333581533883 22.472077438570366 21 22.362549904756936 28.575529055658482 26.53236959528221 22.529551444302378 22 23.905537212994936 28.02127176953903 21.871613277213502 26.201577740252542 23 20.893414597889105 28.700262623259505 26.38124969030276 24.025073088548634 24 22.655331711450675 32.36749309788699 22.740070524560586 22.237104666101743 25 22.706774369393926 26.25253976906685 22.679518311115515 28.361167550423712 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 217.0 1 217.0 2 313.0 3 409.0 4 409.0 5 409.0 6 497.5 7 586.0 8 586.0 9 586.0 10 612.0 11 638.0 12 638.0 13 638.0 14 645.0 15 652.0 16 652.0 17 652.0 18 741.5 19 831.0 20 831.0 21 831.0 22 998.5 23 1166.0 24 1166.0 25 1166.0 26 1487.0 27 1808.0 28 1808.0 29 1808.0 30 2081.5 31 2355.0 32 2355.0 33 2355.0 34 2537.5 35 2720.0 36 2720.0 37 2720.0 38 2972.5 39 3225.0 40 3225.0 41 3225.0 42 3739.5 43 4254.0 44 4254.0 45 4254.0 46 5613.5 47 6973.0 48 6973.0 49 6973.0 50 6136.5 51 5300.0 52 5300.0 53 5300.0 54 4267.5 55 3235.0 56 3235.0 57 3235.0 58 2715.5 59 2196.0 60 2196.0 61 2196.0 62 1941.0 63 1686.0 64 1686.0 65 1686.0 66 1391.0 67 1096.0 68 1096.0 69 1096.0 70 851.5 71 607.0 72 607.0 73 607.0 74 443.0 75 279.0 76 279.0 77 279.0 78 196.5 79 114.0 80 114.0 81 114.0 82 77.0 83 40.0 84 40.0 85 40.0 86 27.5 87 15.0 88 15.0 89 15.0 90 8.5 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.225962133399332 2 38.012622200222744 3 6.397723054077466 4 14.691251082786783 5 14.38930825392897 6 16.040094047766367 7 9.832941467640143 8 8.011384729612672 9 0.11137235490657096 10 1.6606855587179805 11 6.427422348719218 12 6.650167058532359 13 17.567132780596463 14 1.8116569731468877 15 3.125850761044425 16 3.1778245266674916 17 3.145650290805593 18 1.717609206781339 19 1.6557356762776885 20 0.2846182403167925 21 5.152827620344016 22 1.8017572082663036 23 0.10642247246627894 24 9.459225343398096 25 0.11632223734686302 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 40405.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.27744091077837 #Duplication Level Percentage of deduplicated Percentage of total 1 94.0116352598963 73.5899022398218 2 3.452636904009106 5.405271624798911 3 0.9295560895409132 2.182898156168791 4 0.48058682180346524 1.504764261848781 5 0.2592639433413431 1.014725900259869 6 0.17073479195649424 0.801880955327311 7 0.10433792841785759 0.5717114218537309 8 0.09485266219805236 0.5939858928350451 9 0.05374984191222967 0.3786660066823413 >10 0.3572783609459972 5.430021037000371 >50 0.04426457569242443 2.202697685929959 >100 0.03794106487922094 5.0191807944561315 >500 0.003161755406601745 1.3042940230169533 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 527 1.3042940230169533 No Hit GTACATGGGAAGCAGTGGTATCAAC 445 1.1013488429649796 No Hit GAGTACATGGGAAGCAGTGGTATCA 255 0.6311100111372355 No Hit CATGTACTCTGCGTTGATACCACTG 188 0.4652889493874521 No Hit ACGCAGAGTACATGGGAAGCAGTGG 153 0.3786660066823413 No Hit GCGTTGATACCACTGCTTCCCATGT 152 0.3761910654621953 No Hit GTATCAACGCAGAGTACTTTTTTTT 135 0.33411706471971286 No Hit ACTCTGCGTTGATACCACTGCTTCC 128 0.3167924761786908 No Hit GGTATCAACGCAGAGTACATGGGAA 126 0.3118425937383987 No Hit GTATCAACGCAGAGTACATGGGAAG 122 0.30194282885781465 No Hit CNCATGTACTCTGCGTTGATACCAC 112 0.2771934166563544 No Hit TATCAACGCAGAGTACATGGGAAGC 110 0.27224353421606234 No Hit GNACATGGGAAGCAGTGGTATCAAC 102 0.2524440044548942 No Hit GGTATCAACGCAGAGTACTTTTTTT 94 0.23264447469372604 No Hit GCTTCCCATGTACTCTGCGTTGATA 80 0.19799529761168172 No Hit TATCAACGCAGAGTACTTTTTTTTT 73 0.18067070907065957 No Hit GTACTCTGCGTTGATACCACTGCTT 70 0.1732458854102215 No Hit CTGCTTCCCATGTACTCTGCGTTGA 68 0.16829600296992947 No Hit CAGTGGTATCAACGCAGAGTACATG 62 0.15344635564905335 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 61 0.15097141442890732 No Hit ATACCACTGCTTCCCATGTACTCTG 60 0.14849647320876128 No Hit GTGGTATCAACGCAGAGTACATGGG 55 0.13612176710803117 No Hit CCACTGCTTCCCATGTACTCTGCGT 55 0.13612176710803117 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 54 0.13364682588788518 No Hit GATACCACTGCTTCCCATGTACTCT 54 0.13364682588788518 No Hit CATGGGAAGCAGTGGTATCAACGCA 52 0.1286969434475931 No Hit TACCACTGCTTCCCATGTACTCTGC 52 0.1286969434475931 No Hit GGGAAGCAGTGGTATCAACGCAGAG 50 0.12374706100730108 No Hit GCAGAGTACATGGGAAGCAGTGGTA 50 0.12374706100730108 No Hit GNGTACATGGGAAGCAGTGGTATCA 47 0.11632223734686302 No Hit CNTGTACTCTGCGTTGATACCACTG 46 0.113847296126717 No Hit CCATGTACTCTGCGTTGATACCACT 46 0.113847296126717 No Hit AAGCAGTGGTATCAACGCAGAGTAC 44 0.10889741368642494 No Hit GTACTGGTTCACTATCGGTCAGTCA 43 0.10642247246627894 No Hit GTTGATACCACTGCTTCCCATGTAC 41 0.10147259002598688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACACA 30 0.009587456 17.462318 6 CCATGTA 205 7.1468094E-9 10.784515 2 ATGTACT 250 1.8189894E-12 9.897393 4 TGTACTC 265 0.0 9.884331 5 CCCATGT 195 6.7992005E-7 9.676246 1 CATGTAC 265 1.8189894E-12 9.55907 3 GTACTCT 285 0.0 9.558322 6 TACTCTG 265 1.8189894E-12 9.263414 7 TGCGTTG 265 0.0 8.901853 12 TTGATAC 275 0.0 8.893345 16 GATACCA 275 0.0 8.885468 18 TGATACC 275 0.0 8.751241 17 TCTGCGT 270 1.8189894E-12 8.568726 10 CTGCGTT 260 7.2759576E-12 8.542361 11 ATACCAC 290 0.0 8.425874 19 GCGTTGA 280 0.0 8.424968 13 ACTCTGC 280 1.0913936E-11 8.410192 8 CTCTGCG 265 1.2732926E-11 8.381184 9 GTTGATA 295 0.0 8.290406 15 CGTTGAT 295 0.0 7.9981856 14 >>END_MODULE