FastQCFastQC Report
Thu 26 May 2016
SRR1926664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926664_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40405
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC14693.635688652394506No Hit
GTACATGGGAAGCAGTGGTATCAAC13773.4079940601410716No Hit
GTATCAACGCAGAGTACTTTTTTTT6501.608711793094914No Hit
GAGTACATGGGAAGCAGTGGTATCA5431.3438930825392896No Hit
CATGTACTCTGCGTTGATACCACTG5081.2572701398341788No Hit
GGTATCAACGCAGAGTACTTTTTTT4951.2250959039722806No Hit
GCGTTGATACCACTGCTTCCCATGT4391.0864991956441035No Hit
TATCAACGCAGAGTACTTTTTTTTT3830.9479024873159263No Hit
ACGCAGAGTACATGGGAAGCAGTGG3680.910778369013736No Hit
GTATCAACGCAGAGTACATGGGAAG3650.9033535453532979No Hit
TATCAACGCAGAGTACATGGGAAGC3130.7746566019057047No Hit
GGTATCAACGCAGAGTACATGGGAA3070.7598069545848286No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2840.7028833065214701No Hit
ACTCTGCGTTGATACCACTGCTTCC2750.6806088355401559No Hit
GGGAAGCAGTGGTATCAACGCAGAG1660.41084024254423956No Hit
CAGTGGTATCAACGCAGAGTACATG1650.40836530132409354No Hit
GTACTCTGCGTTGATACCACTGCTT1530.3786660066823413No Hit
CATGGGAAGCAGTGGTATCAACGCA1520.3761910654621953No Hit
GCTTCCCATGTACTCTGCGTTGATA1510.37371612424204925No Hit
ATACCACTGCTTCCCATGTACTCTG1450.3588664769211731No Hit
GATACCACTGCTTCCCATGTACTCT1420.35144165326073507No Hit
GCAGTGGTATCAACGCAGAGTACAT1410.34896671204058904No Hit
GTGGTATCAACGCAGAGTACATGGG1360.33659200593985894No Hit
GCAGAGTACATGGGAAGCAGTGGTA1360.33659200593985894No Hit
CCACTGCTTCCCATGTACTCTGCGT1310.32421729983912884No Hit
AAGCAGTGGTATCAACGCAGAGTAC1270.3143175349585447No Hit
CTGCTTCCCATGTACTCTGCGTTGA1230.30441777007796067No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1200.29699294641752255No Hit
ACATGGGAAGCAGTGGTATCAACGC1150.2846182403167925No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1130.2796683578765004No Hit
CCATGTACTCTGCGTTGATACCACT1090.2697685929959164No Hit
GTTGATACCACTGCTTCCCATGTAC1050.25986882811533224No Hit
TACCACTGCTTCCCATGTACTCTGC970.24006929835416407No Hit
GTACTTTTTTTTTTTTTTTTTTTTT960.23759435713401805No Hit
ATGGGAAGCAGTGGTATCAACGCAG960.23759435713401805No Hit
GTACTGGTTCACTATCGGTCAGTCA920.227694592253434No Hit
ATCAACGCAGAGTACTTTTTTTTTT910.22521965103328795No Hit
CTTCCCATGTACTCTGCGTTGATAC840.20789506249226578No Hit
ATCAACGCAGAGTACATGGGAAGCA790.1955203563915357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA780.19304541517138968No Hit
GTACATGGTAAGCAGTGGTATCAAC620.15344635564905335No Hit
AAAAAGTACTCTGCGTTGATACCAC610.15097141442890732No Hit
GGAAGCAGTGGTATCAACGCAGAGT600.14849647320876128No Hit
GTGGTATCAACGCAGAGTACTTTTT510.1262220022274471No Hit
GGATACCACGTGTCCCGCCCTACTC470.11632223734686302No Hit
CCCCATGTACTCTGCGTTGATACCA470.11632223734686302No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA460.113847296126717No Hit
CTATCGGTCAGTCAGGAGTATTTAG420.10394753124613289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC3600.08.69074817
TTGATAC3700.08.45586316
GATACCA4000.07.82167418
CGTTGAT4050.07.734682614
GTTGATA4100.07.630901315
ATACCAC4000.07.58465419
CTGCGTT4100.07.408830611
TGCGTTG4250.07.370697512
GCGTTGA4350.07.201256313
CCATGTA4050.07.04899932
CTCTGCG4250.06.9411919
TCTGCGT4250.06.92398910
AGTGGTA3351.3333192E-96.80064314
CAGTGGT3351.3333192E-96.80064313
TGGTATC3351.369699E-96.792226316
GTGGTAT3351.369699E-96.792226315
ACTCTGC4350.06.78162348
CCCATGT4107.2759576E-126.73093461
ATGTACT4201.2732926E-116.57067444
TGTACTC4201.2732926E-116.57067445
Produced by FastQC (version 0.11.5)