FastQCFastQC Report
Thu 26 May 2016
SRR1926663_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926663_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528732
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7470.1412814053244366No Hit
GTCCTACAGTGTGCATTTCTCATTT5510.10421158545350007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA450.001059458918.1195742
TAGGACC2550.017.5853794
TGTAGGA4450.017.4070032
GTCCTAC4850.016.6882231
GTTTAGG654.700507E-516.3304671
CCTACAC607.0874485E-616.3191363
CTGTAGG4500.016.217061
GTCCTAA2000.015.9222061
CTACACT1153.6379788E-1215.7831474
GTAGGAC4800.015.6391723
TCCTACA5550.015.4261212
TAGGTTG559.244778E-515.0895335
TCCTAAA2600.014.6350392
TCTACAC1351.8189894E-1214.5058983
GATATAC1303.6307028E-914.2891591
TAAGGAG450.00641918513.8320715
TTTAGGA1807.2759576E-1213.589682
TGTAGAA1704.3655746E-1113.5896792
TGCTACA600.007210892613.5896792
TAACCTG704.7672664E-513.3380695
Produced by FastQC (version 0.11.5)