FastQCFastQC Report
Thu 26 May 2016
SRR1926663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926663_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528732
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13870.2623257151070864No Hit
CTGTAGGACGTGGAATATGGCAAGA13450.2543821822776No Hit
GTCCTACAGTGTGCATTTCTCATTT11690.2210949970873713No Hit
CTGTAGGACCTGGAATATGGCGAGA10520.1989665842052306No Hit
CTTTAGGACGTGAAATATGGCGAGG9280.17551424918484224No Hit
GTCCTAAAGTGTGTATTTCTCATTT8040.15206191416445383No Hit
GTATCAACGCAGAGTACTTTTTTTT7650.1446857765370736No Hit
GTACATGGGAAGCAGTGGTATCAAC6650.12577260313353458No Hit
CCCATGTACTCTGCGTTGATACCAC6490.12274649538896833No Hit
GATATACACTGTTCTACAAATCCCG6280.11877472897422513No Hit
GGTATCAACGCAGAGTACTTTTTTT5540.10477898065560626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCC807.879862E-915.40697214
TAGGACC3850.015.3571074
TCCAACG1550.015.28215418
CGGTTTC953.1286618E-1014.97033613
GCCGGTT902.3392204E-914.74855311
CCAACGT653.4587829E-614.57544619
TAGCACT801.2244891E-714.3044034
CCGGTTT955.011316E-913.97231412
GTAGGAC8950.013.7489393
CCAACGA1051.4188117E-913.53434319
GGCGAGG2250.013.4741919
TTTAATC500.001451729813.3545893
TGCCGGT1001.0282747E-813.27495510
TGTAGGA9050.013.1754042
TCCTATA801.9026666E-613.1161152
GTCCTAT957.0280294E-813.0446631
GGACCTG6100.012.9755796
CCGTTTC957.543167E-812.97429213
AGGACCT6500.012.7639285
ATAGCCC603.9452923E-412.71865753