FastQCFastQC Report
Thu 26 May 2016
SRR1926662_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926662_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655972
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC10640.16220204520924672No Hit
GTACATGGGAAGCAGTGGTATCAAC9590.14619526443201844No Hit
GTCCTACAGTGGACATTTCTAAATT8730.1330849487478124No Hit
GTCCTAAAGTGTGTATTTCTCATTT7640.11646838584573732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGG450.001227392617.7283021
GTCCTAA3150.015.6177921
TATACTG601.11543195E-515.5596335
ATAGGAC855.0808012E-815.36801053
GTGTAGG708.707185E-515.1956881
TGTAGGA5300.014.8915872
CTATACT651.8039134E-514.7888114
TCTAGAC450.00466645114.5142323
CAGGAGT601.5140003E-414.2410774
CTGTAGG5250.014.1826421
TAGGACC2000.013.8850514
GCTACAC550.001099809413.8544943
GTCCTAC6050.013.8456581
TAGGACT851.0541189E-613.8222224
TCTAGAA700.001258727313.607832
TTAATCC550.001269513.5937574
ATCTTTG450.007411836613.5358597
CCAACGA450.001488137113.31120319
TCCTACA6750.013.3054332
GGGGGAC550.001582618113.2021145
Produced by FastQC (version 0.11.5)