##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926662_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 655972 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.879465891836848 27.0 10.0 31.0 2.0 31.0 2 16.827840212692006 22.0 2.0 31.0 2.0 31.0 3 20.50568926722482 25.0 15.0 31.0 2.0 31.0 4 22.899303628813424 28.0 7.0 35.0 2.0 37.0 5 23.49593885104852 30.0 10.0 35.0 2.0 37.0 6 24.264733860591612 31.0 10.0 35.0 2.0 37.0 7 23.97883751135719 31.0 10.0 35.0 2.0 37.0 8 24.106567048593536 31.0 13.0 35.0 2.0 37.0 9 26.93094064990579 32.0 17.0 37.0 2.0 39.0 10 27.667982779752794 32.0 25.0 38.0 2.0 39.0 11 24.709043983584664 30.0 10.0 37.0 2.0 39.0 12 25.912092284426773 31.0 17.0 37.0 2.0 39.0 13 22.492595720549048 29.0 2.0 37.0 2.0 39.0 14 25.902492789326374 31.0 17.0 38.0 2.0 40.0 15 27.02255888970871 31.0 18.0 38.0 2.0 40.0 16 27.384859414731118 32.0 21.0 39.0 2.0 40.0 17 26.70017622703408 31.0 17.0 38.0 2.0 40.0 18 26.767869665168636 31.0 17.0 38.0 2.0 40.0 19 27.173385449378937 31.0 21.0 39.0 2.0 40.0 20 27.457342691456343 32.0 24.0 39.0 2.0 40.0 21 25.26293957668925 30.0 10.0 38.0 2.0 40.0 22 26.231308653418132 31.0 16.0 38.0 2.0 40.0 23 26.24117340374284 31.0 10.0 39.0 2.0 40.0 24 25.252933052020513 31.0 2.0 39.0 2.0 40.0 25 25.054953565091193 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.862569404884347 1101 2 10.752329337246795 1101 3 6.992749941660524 1101 4 8.720609096152977 1101 5 8.420166310915981 1101 6 8.191254072637648 1101 7 7.890993615712674 1101 8 6.044369622784611 1101 9 5.9720205727781455 1101 10 5.697419127134101 1101 11 8.444254275405616 1101 12 6.1145243938038085 1101 13 10.782903507896076 1101 14 7.979338804331739 1101 15 7.082954820582799 1101 16 6.7942989702236005 1101 17 2.2375576791665743 1101 18 4.937899194239755 1101 19 5.618423997864415 1101 20 3.740500905101719 1101 21 0.49517776757346965 1101 22 4.689594732445684 1101 23 5.818244205151682 1101 24 7.373495313116852 1101 25 7.701647578639726 1102 1 6.149919740133388 1102 2 11.002245524986257 1102 3 7.3011072213540125 1102 4 8.609216615310064 1102 5 8.244537718961958 1102 6 7.401006227810061 1102 7 7.405858318777806 1102 8 7.412999890983844 1102 9 6.588244231781982 1102 10 5.729004376176253 1102 11 8.53789651678493 1102 12 5.903479135183119 1102 13 10.423971515558915 1102 14 8.026147951841317 1102 15 6.115767320582798 1102 16 6.416156010453491 1102 17 7.260869460775766 1102 18 7.108804366653551 1102 19 5.687150051504265 1102 20 6.059167092841179 1102 21 4.756163160293774 1102 22 6.129034387618098 1102 23 6.612586877565473 1102 24 7.9344807058371565 1102 25 7.738596812356196 1103 1 4.623782863157029 1103 2 9.097490969949366 1103 3 6.040254187172412 1103 4 7.660620771310668 1103 5 7.138627464482212 1103 6 6.669945393598347 1103 7 6.557074250113448 1103 8 6.185824013856905 1103 9 5.061896543180865 1103 10 4.42018416837622 1103 11 5.866920760121772 1103 12 4.113180234415161 1103 13 8.28503536136527 1103 14 6.403346415928048 1103 15 5.758452910102449 1103 16 5.369028318840776 1103 17 2.9297747461243624 1103 18 1.6789745217506713 1103 19 3.67693321669023 1103 20 4.017076293262804 1103 21 6.1964658255550304 1103 22 1.9366198416159897 1103 23 4.2217308317892375 1103 24 5.697709135290552 1103 25 1.7479327557382192 1104 1 5.818232307768145 1104 2 10.542021343184029 1104 3 7.243253546410312 1104 4 8.037528890470195 1104 5 7.787883921178917 1104 6 7.433984935657154 1104 7 7.093237307400543 1104 8 7.129664364090804 1104 9 5.894531705093666 1104 10 4.993035115259634 1104 11 7.936713456915371 1104 12 4.863046711445879 1104 13 9.285580564723901 1104 14 6.876079951490347 1104 15 6.041997176392805 1104 16 5.729261067339806 1104 17 6.2214555799299305 1104 18 3.8387420695578207 1104 19 4.634804404989097 1104 20 3.9194263715987496 1104 21 2.9170461411951827 1104 22 4.8473820522081965 1104 23 5.392121696669918 1104 24 6.507619836017614 1104 25 5.366750420030733 1105 1 3.488736482147985 1105 2 9.562691555184525 1105 3 6.472871692588832 1105 4 5.98579448961199 1105 5 2.108797684095073 1105 6 4.839249734591377 1105 7 5.99517590291633 1105 8 3.248007868999977 1105 9 3.9608050272197346 1105 10 3.406993177405454 1105 11 6.938403268550431 1105 12 4.26424724084751 1105 13 5.2016677895299 1105 14 3.800084216356872 1105 15 4.525643070368574 1105 16 4.605172202871419 1105 17 5.209668326124586 1105 18 2.4235193741883414 1105 19 2.348269042137481 1105 20 1.7798958993890643 1105 21 2.937138546636593 1105 22 4.249068811280303 1105 23 1.6215392458543398 1105 24 4.8280953381097085 1105 25 5.544719201033324 1106 1 6.405472393099835 1106 2 11.15642235071481 1106 3 7.854808022468607 1106 4 9.149507454738835 1106 5 8.583229231791403 1106 6 7.894878862199938 1106 7 7.484927748709303 1106 8 7.272273663188653 1106 9 6.117801296683869 1106 10 5.3669267028916785 1106 11 7.898641901668242 1106 12 5.125756506598421 1106 13 4.276064282306855 1106 14 5.453533880089314 1106 15 5.684201664017145 1106 16 5.735455325442459 1106 17 6.313893958627851 1106 18 6.319317544408108 1106 19 5.9717489136893285 1106 20 5.481189036414847 1106 21 6.312386311344511 1106 22 6.285869984970937 1106 23 6.683116890336866 1106 24 7.677235843419883 1106 25 6.73605414429629 1107 1 4.452668270448385 1107 2 -14.134823440530983 1107 3 -5.603226521443801 1107 4 2.388673928638134 1107 5 5.07796025705678 1107 6 5.186317420814067 1107 7 5.0448912514209 1107 8 5.6004022775768085 1107 9 3.9801975235839144 1107 10 3.0676746402808384 1107 11 5.912964517135894 1107 12 3.2710195698004156 1107 13 5.110856222060626 1107 14 4.395163920955994 1107 15 3.9385196530679565 1107 16 3.5825265465594818 1107 17 4.434347861524504 1107 18 -0.4598160305269978 1107 19 2.225135193793168 1107 20 2.58694121468611 1107 21 -0.714845292222968 1107 22 2.2502658520385594 1107 23 -0.6579207121511175 1107 24 2.793455289792 1107 25 3.443934474314023 1108 1 5.140177061221589 1108 2 10.693484767174208 1108 3 0.39530089085092257 1108 4 5.949352640262415 1108 5 7.252806072222516 1108 6 7.2703081974282675 1108 7 7.092857972217416 1108 8 6.969167221891254 1108 9 5.269802887127671 1108 10 4.642648747457944 1108 11 7.1295819554726165 1108 12 2.517568258962939 1108 13 3.2865502136470575 1108 14 4.654437387525483 1108 15 4.4793770358256815 1108 16 4.478226419972348 1108 17 0.030245074476454192 1108 18 3.2409247386283653 1108 19 4.357152361906849 1108 20 4.657525263002331 1108 21 4.585756529438349 1108 22 1.7548690205528885 1108 23 4.236206412019353 1108 24 5.237524661246386 1108 25 5.711715278248878 1109 1 3.641885076362911 1109 2 -14.134823440530983 1109 3 -5.877574861489082 1109 4 2.0907903625571755 1109 5 2.0175594102160694 1109 6 2.629813510520414 1109 7 3.6283965575324437 1109 8 4.0573289403699135 1109 9 3.2935018719907454 1109 10 2.460460465716782 1109 11 0.803656615738074 1109 12 1.3850091378982974 1109 13 2.82995622005706 1109 14 3.294224248338736 1109 15 3.2696824561908464 1109 16 3.1199565052192284 1109 17 3.583426554932103 1109 18 3.8118876019387926 1109 19 2.264585415187252 1109 20 1.183209238435051 1109 21 0.48515070064433274 1109 22 2.6123036125856665 1109 23 3.1236972344605185 1109 24 4.244264396668907 1109 25 4.568910264553985 1110 1 3.462438812455634 1110 2 9.437742931150432 1110 3 6.470705939693957 1110 4 7.838168400479429 1110 5 8.413091274042827 1110 6 4.110626307150923 1110 7 6.409649896440303 1110 8 7.316333732912437 1110 9 5.389716615059367 1110 10 5.063342185147871 1110 11 3.270166886025084 1110 12 5.7673866725648715 1110 13 10.37603895529038 1110 14 7.924187317114431 1110 15 6.919970860405805 1110 16 6.596755644264903 1110 17 7.249181941703448 1110 18 7.122689017248604 1110 19 6.096533634049276 1110 20 6.188027330863761 1110 21 4.1325287385665845 1110 22 6.299273935985504 1110 23 6.845276837895046 1110 24 7.623678142517047 1110 25 8.125658148944542 1111 1 4.920732122984241 1111 2 9.40874860433528 1111 3 7.306475255628506 1111 4 7.858388761333689 1111 5 8.091746965934675 1111 6 7.198931758142393 1111 7 7.232096008819756 1111 8 7.373131246789981 1111 9 5.777967090369938 1111 10 4.930753059642971 1111 11 6.8178926490035465 1111 12 6.035610729006571 1111 13 7.625059700974404 1111 14 5.818433959398366 1111 15 5.526056362860626 1111 16 5.125562901054778 1111 17 6.461381198004652 1111 18 6.262721239106021 1111 19 5.195651732501556 1111 20 4.793409602733778 1111 21 7.845501472155963 1111 22 6.984811514154053 1111 23 6.549053361752197 1111 24 6.333611942634633 1111 25 7.141685087028179 1112 1 -2.1599035889428144 1112 2 7.841367035659491 1112 3 6.25592234024959 1112 4 -0.27124771601633313 1112 5 4.7470098955720665 1112 6 4.025951493272569 1112 7 6.444523708305265 1112 8 6.519347576929231 1112 9 5.187105449321358 1112 10 3.7744142770459206 1112 11 0.2781111978042041 1112 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6.53660330406176 2204 5 1.629457158805355 2204 6 4.710993428532845 2204 7 5.70903931561563 2204 8 5.468793446171318 2204 9 3.9605864995127433 2204 10 3.6559920286382273 2204 11 4.904475345274612 2204 12 5.208376740965381 2204 13 3.719096496736448 2204 14 4.446141175753375 2204 15 4.748845923202886 2204 16 4.626670240238159 2204 17 -0.8090898826966004 2204 18 3.052773648388225 2204 19 3.7930904219304047 2204 20 4.1961155945728486 2204 21 5.647994209834508 2204 22 4.840549972620355 2204 23 3.889853102531596 2204 24 5.550110882015442 2204 25 5.730465506829923 2205 1 6.309540085820895 2205 2 10.802001164622798 2205 3 7.751209695054818 2205 4 8.570985325717952 2205 5 8.491946495870263 2205 6 8.020944170310134 2205 7 8.054895925909403 2205 8 7.496901487567101 2205 9 6.3677803053910615 2205 10 5.731041668004149 2205 11 8.194449100842025 2205 12 7.05223870178386 2205 13 10.307070520919076 2205 14 7.5788015190726625 2205 15 6.81354371293525 2205 16 6.388133394579377 2205 17 7.201099345833242 2205 18 6.941925586592205 2205 19 4.972105765667131 2205 20 5.6639518264095585 2205 21 -0.41204419488150634 2205 22 4.289163518813098 2205 23 5.630309095010368 2205 24 7.591585071360132 2205 25 7.946651388559371 2206 1 4.4097673334016 2206 2 9.361763590185742 2206 3 6.369657886294579 2206 4 2.561020193043383 2206 5 6.2590546072754805 2206 6 6.39285923475369 2206 7 6.714464297358482 2206 8 6.468881378567698 2206 9 5.103034746081143 2206 10 4.271734163159891 2206 11 4.100024326600156 2206 12 5.137535272643397 2206 13 8.524027417642003 2206 14 0.8408972751318871 2206 15 2.931211006589624 2206 16 3.9840476192565966 2206 17 4.946264306015266 2206 18 -0.6963798159991548 2206 19 1.169879250044925 2206 20 2.7726100727164322 2206 21 3.8888276301066504 2206 22 -1.2772354723324746 2206 23 -2.4781775098653824 2206 24 1.5563943224077121 2206 25 3.361334249098576 2207 1 1.4909221285495704 2207 2 -14.134823440530983 2207 3 -6.283215065554481 2207 4 -4.633248678017296 2207 5 0.9956871442493167 2207 6 1.743844711165785 2207 7 3.137501101282659 2207 8 3.2519273428423325 2207 9 0.6258580547406289 2207 10 0.22262655859153568 2207 11 -1.8867116336852945 2207 12 -2.68638875554684 2207 13 0.48137933762190954 2207 14 -6.154546927991497 2207 15 -1.7564188266033476 2207 16 -0.8392440492569158 2207 17 0.4054283501622429 2207 18 0.4637379388284977 2207 19 -0.2688956269552101 2207 20 -0.5954343402085271 2207 21 -4.1964640975058956 2207 22 -4.854317605216654 2207 23 -1.9174362602162844 2207 24 -0.05256213637809992 2207 25 0.4276847359268814 2208 1 4.795694748668726 2208 2 6.7224372855416235 2208 3 6.3180043421005685 2208 4 8.278270331026466 2208 5 3.7993175072856182 2208 6 6.360267067687154 2208 7 6.86815773837494 2208 8 6.736270062828616 2208 9 4.949451128333703 2208 10 4.647329743195709 2208 11 6.535580595537628 2208 12 6.125173114925921 2208 13 8.785206863231615 2208 14 3.398678394540756 2208 15 4.720305274378177 2208 16 5.092282674844064 2208 17 5.981627398638523 2208 18 0.7991821975400839 2208 19 3.4079592013847666 2208 20 4.498665784089617 2208 21 6.831046510697782 2208 22 6.131529550927532 2208 23 2.2652584378249507 2208 24 3.5492980496405018 2208 25 5.253180856967557 2209 1 3.180465918093361 2209 2 5.125666069958527 2209 3 5.398611441272024 2209 4 1.0765665067978887 2209 5 5.3508736244357955 2209 6 5.581715174036248 2209 7 6.182739196520753 2209 8 4.908345924260914 2209 9 4.411863715746289 2209 10 3.508049469014445 2209 11 -0.5562920532657145 2209 12 3.209640215482132 2209 13 2.915212171454744 2209 14 3.9668400183954518 2209 15 4.449158928974409 2209 16 3.7511572161443496 2209 17 1.49451426424816 2209 18 3.4691491942397548 2209 19 3.8826528245932437 2209 20 4.10181840704422 2209 21 6.103793977752179 2209 22 5.046456620557571 2209 23 0.9227568799768555 2209 24 -2.553727637543602 2209 25 0.5916291248712682 2210 1 -1.938700629644618 2210 2 -14.134823440530983 2210 3 -7.779442647496349 2210 4 -2.073693947033995 2210 5 -0.4687531773338627 2210 6 -1.3121231305163406 2210 7 -0.9290418795460376 2210 8 -21.3792414883265 2210 9 -13.294055365172792 2210 10 -7.210892303498756 2210 11 -10.381145770976573 2210 12 -4.582121594136822 2210 13 -5.361725176911175 2210 14 -4.1396427845159245 2210 15 -6.072497985539648 2210 16 -5.45618900905902 2210 17 -4.902178328873006 2210 18 -5.141704238042248 2210 19 -10.377801007289161 2210 20 -7.313801687147304 2210 21 -5.4706137573698435 2210 22 -9.948566213750976 2210 23 -7.895853081490248 2210 24 -10.62986578511032 2210 25 -8.29136288312074 2211 1 0.21772111572591513 2211 2 4.856191833503157 2211 3 0.5404719392645987 2211 4 1.6255277656648062 2211 5 1.2104220948333229 2211 6 0.09049177479398196 2211 7 -21.203624439842883 2211 8 -13.815000697670614 2211 9 -9.681501252618677 2211 10 -7.443631711440318 2211 11 -9.90574291686752 2211 12 -4.925268115180916 2211 13 -6.779194051253366 2211 14 -4.962894555207047 2211 15 -5.462242562615764 2211 16 -12.060464338401735 2211 17 -7.757570914723814 2211 18 -6.023887643136707 2211 19 -9.040961297134086 2211 20 -6.864110318318467 2211 21 -4.474271824370227 2211 22 -6.023932895586661 2211 23 -7.736794260706358 2211 24 -22.658622742438705 2211 25 -22.44071353247139 2212 1 -1.3398490382681096 2212 2 -14.134823440530983 2212 3 -8.021219696582369 2212 4 -20.181300626069245 2212 5 -20.67097952241735 2212 6 -21.394683427362352 2212 7 -10.19684149410645 2212 8 -9.615675596853631 2212 9 -5.33058763135622 2212 10 -5.728533715243156 2212 11 -5.859403670330817 2212 12 -3.0325807418551065 2212 13 -6.47444468331839 2212 14 -3.8282960470894523 2212 15 -3.987721051510224 2212 16 -4.104259653807404 2212 17 -4.272737863469086 2212 18 -3.3341453794036546 2212 19 -3.844592798001216 2212 20 -4.065498771900867 2212 21 -1.7743433887572806 2212 22 -3.272856817941914 2212 23 -3.4197039537630474 2212 24 -2.247770029260412 2212 25 -2.8728840751070486 2213 1 -19.221631984004542 2213 2 -14.134823440530983 2213 3 -8.681590999599205 2213 4 -5.338413152608524 2213 5 -20.67097952241735 2213 6 -10.875575147107575 2213 7 -21.203624439842883 2213 8 -10.813424078560045 2213 9 -8.35999685468116 2213 10 -7.109397044132706 2213 11 -6.834308622683558 2213 12 -5.0894797197843005 2213 13 -19.63723538099281 2213 14 -12.617623554125448 2213 15 -6.863585121030788 2213 16 -6.138871810031173 2213 17 -4.63329555862536 2213 18 -4.779789234634979 2213 19 -5.930629434548891 2213 20 -5.975672573313002 2213 21 -4.0491483559226396 2213 22 -4.884383505346246 2213 23 -4.793469568733666 2213 24 -3.5928053326722527 2213 25 -4.476806950730413 2214 1 -19.221631984004542 2214 2 -14.134823440530983 2214 3 -11.862291709169323 2214 4 -20.181300626069245 2214 5 -20.67097952241735 2214 6 -21.394683427362352 2214 7 -16.35755830529328 2214 8 -14.503528831542008 2214 9 -12.9926811407655 2214 10 -12.78757152914361 2214 11 -13.775796323226084 2214 12 -11.227916099354687 2214 13 -19.63723538099281 2214 14 -16.407793187623163 2214 15 -24.4092326794172 2214 16 -17.964309329852412 2214 17 -24.132233987500094 2214 18 -17.5545110110054 2214 19 -14.11783756301231 2214 20 -12.63371715203752 2214 21 -10.156381565063045 2214 22 -11.313006464817715 2214 23 -11.144314589033039 2214 24 -9.973331978470632 2214 25 -9.651659940681194 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 105502.0 3 6.0 4 631.0 5 2194.0 6 3015.0 7 2955.0 8 2348.0 9 2226.0 10 2090.0 11 1965.0 12 1889.0 13 1965.0 14 3082.0 15 6526.0 16 9902.0 17 10389.0 18 10498.0 19 11975.0 20 14133.0 21 16615.0 22 17561.0 23 19426.0 24 20558.0 25 21258.0 26 17873.0 27 14034.0 28 14684.0 29 17214.0 30 16649.0 31 18957.0 32 24717.0 33 31547.0 34 38959.0 35 45127.0 36 45204.0 37 46965.0 38 35333.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.273542702727255 19.272954036902533 12.548888085368773 25.90461517500144 2 12.881471518687606 21.280711890734814 42.33220936179886 23.50560722877872 3 18.320197718508737 25.482985420675107 29.306133621001955 26.890683239814194 4 10.78020134228188 17.45633371710988 40.359895818707706 31.40356912190054 5 11.356245471188885 38.27497365850927 38.189210665415814 12.17957020488604 6 30.49939249891359 38.29775535000044 18.98099452805591 12.221857623030056 7 25.993829984399007 32.42492337752218 23.709147944640783 17.872098693438033 8 23.337398996258603 38.9060897704958 21.11692155245775 16.63958968078785 9 26.325428633348697 15.374941622839284 20.63834852920079 37.66128121461123 10 13.734835154479432 30.206410290080534 36.03721904579463 20.021535509645403 11 32.4117463292721 22.401826748728652 25.82955712937645 19.356869792622796 12 23.373580506154628 25.0906608094094 33.19779837551289 18.337960308923083 13 28.29452413378694 22.08625159443652 25.47302366332697 24.14620060844957 14 19.354179914317672 23.526435735935056 28.643239404586744 28.476144945160524 15 22.085326928744774 32.761383090130366 23.696611880679004 21.456678100445856 16 20.029079707258006 28.53654098921196 30.40507687143758 21.029302432092454 17 20.642238495133537 29.11040315415197 29.97572644375947 20.271631906955022 18 20.400330181971075 27.72601079583748 32.32552603429193 19.54813298789951 19 22.26676925358745 27.467648040342617 27.928362193629724 22.337220512440208 20 22.505679507602743 28.444930943914287 29.06746001412615 19.981929534356816 21 21.098153794307187 26.95279092761742 27.32715070087144 24.621904577203953 22 21.760704814187882 29.49227755617378 27.919473679324398 20.82754395031394 23 20.42394856086725 29.128492523896515 29.280197063280937 21.167361851955295 24 22.006146893712888 28.249639425688265 29.295680963180477 20.44853271741837 25 21.55057287588732 28.183784262282067 28.870289055453043 21.395353806377564 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 648.0 1 648.0 2 1339.5 3 2031.0 4 2031.0 5 2031.0 6 2991.0 7 3951.0 8 3951.0 9 3951.0 10 4615.0 11 5279.0 12 5279.0 13 5279.0 14 6923.5 15 8568.0 16 8568.0 17 8568.0 18 12378.0 19 16188.0 20 16188.0 21 16188.0 22 22710.0 23 29232.0 24 29232.0 25 29232.0 26 38203.5 27 47175.0 28 47175.0 29 47175.0 30 56642.5 31 66110.0 32 66110.0 33 66110.0 34 71907.0 35 77704.0 36 77704.0 37 77704.0 38 80095.5 39 82487.0 40 82487.0 41 82487.0 42 81980.5 43 81474.0 44 81474.0 45 81474.0 46 80671.5 47 79869.0 48 79869.0 49 79869.0 50 71389.0 51 62909.0 52 62909.0 53 62909.0 54 52036.0 55 41163.0 56 41163.0 57 41163.0 58 33337.5 59 25512.0 60 25512.0 61 25512.0 62 19778.5 63 14045.0 64 14045.0 65 14045.0 66 10505.5 67 6966.0 68 6966.0 69 6966.0 70 5012.5 71 3059.0 72 3059.0 73 3059.0 74 2081.5 75 1104.0 76 1104.0 77 1104.0 78 732.5 79 361.0 80 361.0 81 361.0 82 229.5 83 98.0 84 98.0 85 98.0 86 63.5 87 29.0 88 29.0 89 29.0 90 18.5 91 8.0 92 8.0 93 8.0 94 4.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.727829846395883 2 35.62042282292537 3 5.31836724738251 4 13.140195008323527 5 12.901465306446006 6 14.053496185812808 7 9.0270621307007 8 7.385223759550713 9 0.11433414840877354 10 1.3215503100742105 11 5.332239790722775 12 5.331934899660351 13 15.264828376820963 14 1.468202911099864 15 2.758654332806888 16 2.807132011732208 17 2.7179513759733647 18 1.3805467306531376 19 1.3287152500411603 20 0.28446336124102856 21 4.100479898532254 22 1.474758068941967 23 0.11448659393998525 24 8.784063953949255 25 0.11723061350179581 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 655972.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.13606742868714 #Duplication Level Percentage of deduplicated Percentage of total 1 96.88741471599025 88.29937960547663 2 2.2194716276509068 4.045478318273021 3 0.3953398874664911 1.0808916792418715 4 0.15495289530277398 0.5648719005833562 5 0.07588500607039615 0.34579305150289785 6 0.05234414403592702 0.2862263664213094 7 0.03507109947847539 0.22373694608079694 8 0.028427720175096104 0.20726324981771269 9 0.020614762744806947 0.1690873566783402 >10 0.11111270992531959 1.9094946176603727 >50 0.00899115144416363 0.5953372274438861 >100 0.009163947083377819 1.4156853268604466 >500 0.0010374279703485782 0.6890908202804089 >1k 1.7290466172476305E-4 0.16766353367902015 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 1064 0.16220204520924672 No Hit GTACATGGGAAGCAGTGGTATCAAC 959 0.14619526443201844 No Hit GTCCTACAGTGGACATTTCTAAATT 873 0.1330849487478124 No Hit GTCCTAAAGTGTGTATTTCTCATTT 764 0.11646838584573732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCTAGG 45 0.0012273926 17.728302 1 GTCCTAA 315 0.0 15.617792 1 TATACTG 60 1.11543195E-5 15.559633 5 ATAGGAC 85 5.0808012E-8 15.3680105 3 GTGTAGG 70 8.707185E-5 15.195688 1 TGTAGGA 530 0.0 14.891587 2 CTATACT 65 1.8039134E-5 14.788811 4 TCTAGAC 45 0.004666451 14.514232 3 CAGGAGT 60 1.5140003E-4 14.241077 4 CTGTAGG 525 0.0 14.182642 1 TAGGACC 200 0.0 13.885051 4 GCTACAC 55 0.0010998094 13.854494 3 GTCCTAC 605 0.0 13.845658 1 TAGGACT 85 1.0541189E-6 13.822222 4 TCTAGAA 70 0.0012587273 13.60783 2 TTAATCC 55 0.0012695 13.593757 4 ATCTTTG 45 0.0074118366 13.535859 7 CCAACGA 45 0.0014881371 13.311203 19 TCCTACA 675 0.0 13.305433 2 GGGGGAC 55 0.0015826181 13.202114 5 >>END_MODULE