FastQCFastQC Report
Thu 26 May 2016
SRR1926662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926662_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655972
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC27250.41541407255187723No Hit
GTACATGGGAAGCAGTGGTATCAAC25200.3841627386534791No Hit
GTCCTACAGTGGACATTTCTAAATT17150.2614440860280622No Hit
CTGTAGGACGTGGAATATGGCAAGA16620.2533644728738422No Hit
CTTTAGGACGTGAAATATGGCGAGG15670.23888214740873087No Hit
GTCCTAAAGTGTGTATTTCTCATTT15570.2373576920966139No Hit
GTCCTACAGTGTGCATTTCTCATTT12290.18735555785917693No Hit
CTGTAGGACCTGGAATATGGCGAGA11320.1725683413316422No Hit
GAGTACATGGGAAGCAGTGGTATCA9570.14589037336959504No Hit
GTATCAACGCAGAGTACTTTTTTTT9300.1417743440268792No Hit
CATGTACTCTGCGTTGATACCACTG9030.13765831468416334No Hit
CTGAAGGACCTGGAATATGGCGAGA7910.12058441518845316No Hit
GCGTTGATACCACTGCTTCCCATGT6850.10442518888001318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG807.943527E-915.3991618
TAGGACC4250.014.7997884
GTCTTAG653.2533844E-614.662921
CCAACGA602.6265036E-514.21352719
TCTAGGA1153.5470293E-1013.2644472
AGGACCT7150.012.9290385
GGCGAGG3500.012.7244919
GGACCTG7150.012.5291716
GTTATAT1001.3779027E-712.3901661
TGTAGGA11200.012.3428942
TAAGACC701.057269E-412.2530724
TGGCGAG10450.012.15156518
CCCTGTA1103.6148776E-812.1339552
CTAAGCT550.002993234712.1293034
CTGTAGG10850.012.1222491
GGCGAGA6800.011.98395319
GTAGGAC10700.011.7577433
GCCTATG657.797143E-411.7303361
AAAGGCG657.802162E-411.7294365
ATAGGAC1001.8500432E-611.4370773
Produced by FastQC (version 0.11.5)