FastQCFastQC Report
Thu 26 May 2016
SRR1926661_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926661_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628877
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9310.1480416679255244No Hit
GTCCTAAAGTGTGTATTTCTCATTT6880.10940136147450139No Hit
CTGTAGGACGTGGAATATGGCAAGA6820.10844727983373537No Hit
GTCCTACAGTGTGCATTTCTCATTT6380.10145068113478471No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG552.2753465E-417.0457741
TCTAGGA500.002011867516.4662152
GCATTAT500.002365744716.071731
CGCTCAC350.002698865515.75947210
TAGGACC2250.014.7855194
TGTAGGA4950.014.6920772
ATTATAC601.2173789E-414.61047653
GTCCTAA2950.014.5281181
TTAGGAC3100.014.4926513
GGACCTG3600.014.4844216
GTATTGA658.106019E-414.4233481
TTTAGGA3350.013.9266492
TAGGACA1550.013.8474
AGGACCT3850.013.8177835
GGACGTG5100.013.7005586
GTCCTAC6550.013.69982051
TAGGACT1106.884875E-913.6581764
TCCTACA7100.013.5285812
GTAGGAC6000.013.5146923
GTGTAGG802.5456256E-413.3931091
Produced by FastQC (version 0.11.5)