FastQCFastQC Report
Thu 26 May 2016
SRR1926661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926661_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628877
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA17850.2838392881278851No Hit
GTCCTACAGTGGACATTTCTAAATT17570.2793869071376438No Hit
CTTTAGGACGTGAAATATGGCGAGG15930.2533086756233731No Hit
GTCCTAAAGTGTGTATTTCTCATTT15250.24249575036135843No Hit
GTATCAACGCAGAGTACTTTTTTTT13300.2114880970364634No Hit
GTCCTACAGTGTGCATTTCTCATTT11840.18827211044449074No Hit
CTGTAGGACCTGGAATATGGCGAGA10910.17348384501261774No Hit
CTGAAGGACCTGGAATATGGCGAGA9510.15122194006141107No Hit
GGTATCAACGCAGAGTACTTTTTTT8290.13182228003250238No Hit
CCCATGTACTCTGCGTTGATACCAC8050.12800595346943838No Hit
GTACATGGGAAGCAGTGGTATCAAC7740.12307653165881405No Hit
TATCAACGCAGAGTACTTTTTTTTT7510.11941921870254436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGG350.00211339416.3483031
ATGTCGA456.911157E-414.73312517
TAGACAG653.2196294E-614.6774285
TCTAGAC400.00512007214.3139322
TGTCGAG400.00538142914.20694318
TAGGACC4300.013.5339544
GGCGTGC500.001494209913.3042448
AAAGGCG751.4123543E-512.7204375
TATGTCG604.1916448E-412.62839316
TTAGGAC6250.012.2116193
CGCCACC550.003116579512.06396714
AGGACCT7200.011.9254115
GAAATGT2000.011.9244556
GACGTGG4350.011.7987497
AGGACGT9750.011.7419425
TAGGACT1302.4319888E-911.7419424
TGTACTG1302.4319888E-911.7419425
GGCGAGG4600.011.73523619
GGACGTG9350.011.7331546
CCTACAC907.0977167E-611.6604013
Produced by FastQC (version 0.11.5)