##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926661_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628877 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.40687765652107 31.0 28.0 31.0 23.0 34.0 2 28.856215126328358 31.0 28.0 33.0 23.0 34.0 3 29.245828675559768 31.0 30.0 33.0 25.0 34.0 4 32.98452956619498 35.0 33.0 37.0 27.0 37.0 5 32.72384265921635 35.0 33.0 37.0 26.0 37.0 6 32.80958597627199 35.0 33.0 37.0 26.0 37.0 7 32.63573958023588 35.0 33.0 37.0 25.0 37.0 8 32.59883252209892 35.0 33.0 37.0 25.0 37.0 9 33.79307082307033 37.0 34.0 39.0 23.0 39.0 10 33.6982096658011 37.0 33.0 39.0 23.0 39.0 11 33.933918715424475 37.0 34.0 39.0 25.0 39.0 12 33.74609025294294 37.0 34.0 39.0 23.0 39.0 13 33.89130465893967 37.0 34.0 39.0 25.0 39.0 14 34.52912254701635 38.0 34.0 40.0 22.0 41.0 15 34.67876707209836 39.0 34.0 40.0 24.0 41.0 16 34.66252383216432 39.0 34.0 40.0 24.0 41.0 17 34.58813567676986 39.0 34.0 40.0 23.0 41.0 18 34.55126360162639 39.0 34.0 40.0 23.0 41.0 19 34.595475744859485 39.0 34.0 40.0 22.0 41.0 20 34.58224104236599 39.0 34.0 40.0 22.0 41.0 21 34.49832638178849 39.0 34.0 40.0 19.0 41.0 22 34.44686003781344 39.0 34.0 40.0 19.0 41.0 23 34.09548131033573 39.0 34.0 40.0 18.0 41.0 24 34.185220639330105 39.0 34.0 41.0 18.0 41.0 25 33.98808352030683 39.0 34.0 40.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8573382982009932 1101 2 0.6598801092816515 1101 3 0.7213747093583471 1101 4 0.6934087197840952 1101 5 0.699657625279535 1101 6 0.2882606792715805 1101 7 0.6443756326705099 1101 8 0.4952222830685997 1101 9 0.8414662259391079 1101 10 0.8114601942023754 1101 11 0.7683783756503786 1101 12 0.8751590995084086 1101 13 0.7033940120256545 1101 14 0.7642883307465809 1101 15 0.8084645295059971 1101 16 0.8171948092557102 1101 17 0.7284233906536528 1101 18 1.0879273643576184 1101 19 1.0435386635161308 1101 20 0.8753991115652582 1101 21 0.9666326138854799 1101 22 0.9251618576337322 1101 23 1.2257257201733864 1101 24 1.2155942363250887 1101 25 1.3917390908427052 1102 1 0.27396598722523535 1102 2 0.37662351859540166 1102 3 0.1774702929316092 1102 4 0.02546358644590896 1102 5 0.25976548074697803 1102 6 0.010281661369582196 1102 7 0.17274182848725417 1102 8 0.23927602717431284 1102 9 0.4941884244860262 1102 10 0.63596205993219 1102 11 0.1836652337752298 1102 12 0.287458537883893 1102 13 0.09475639323046181 1102 14 0.36145872714053695 1102 15 0.4164603164559324 1102 16 0.21081616138145876 1102 17 0.4096365134730746 1102 18 0.5890204706851563 1102 19 0.5372424750098546 1102 20 0.4513570087820824 1102 21 0.38586314325382887 1102 22 0.31462299605806976 1102 23 0.5985260818859075 1102 24 0.34067089235519177 1102 25 0.44059020317800446 1103 1 -0.05127942598948465 1103 2 -0.1689386656349079 1103 3 -0.12855664806381384 1103 4 -0.3150687687479987 1103 5 -0.16338698111471928 1103 6 -0.2949565141755244 1103 7 -0.12879318988799326 1103 8 -0.15792095689578645 1103 9 0.17561326163846047 1103 10 0.15050766779103242 1103 11 -0.029146846808849602 1103 12 0.1523301922184146 1103 13 0.009255272445706453 1103 14 0.44084120635859136 1103 15 0.28610145452677926 1103 16 0.4327824886506093 1103 17 0.2707119046937052 1103 18 0.1332089030621333 1103 19 0.37692984984384736 1103 20 -0.08247031306935781 1103 21 -0.0760811196633 1103 22 0.07389669942604371 1103 23 0.45146260822610884 1103 24 0.2505724459947558 1103 25 0.3052615918970787 1104 1 -0.2337410532235218 1104 2 0.027509751568519647 1104 3 -0.06822293175682148 1104 4 -0.3111004673585356 1104 5 0.022502630283668168 1104 6 -0.2745678613493965 1104 7 -0.13593596167267918 1104 8 -0.14981790568258901 1104 9 0.11276660076476475 1104 10 0.12655616488971333 1104 11 -0.005785168872058932 1104 12 0.051540116983723294 1104 13 0.00488834839569563 1104 14 0.22666645769572824 1104 15 0.27944437073748674 1104 16 -0.11588959427655965 1104 17 -0.05674081968304989 1104 18 -0.019373949846958283 1104 19 0.09078706610901577 1104 20 0.025450583806019722 1104 21 0.22453926352660147 1104 22 0.038481594102592 1104 23 0.19991805211420655 1104 24 0.14248766143024483 1104 25 0.37920225431063415 1105 1 0.6297712306709293 1105 2 0.584658465845159 1105 3 0.5069649296221463 1105 4 0.45078642066378904 1105 5 0.4323939268303505 1105 6 0.24756014259715187 1105 7 0.32381686728169257 1105 8 0.2902368048966295 1105 9 0.7607520354754627 1105 10 0.7784386559369594 1105 11 0.6147707308703119 1105 12 0.45864556271572354 1105 13 0.7026651948983726 1105 14 0.886046079984844 1105 15 0.5219404336711548 1105 16 0.5414763289190958 1105 17 0.4301610617768148 1105 18 0.7868126683347398 1105 19 0.7023667020922773 1105 20 0.5792343334770749 1105 21 0.4308044167978906 1105 22 0.8366076412714918 1105 23 0.8836295280541719 1105 24 0.7271500554140573 1105 25 0.8984825363110005 1106 1 0.24702190452537565 1106 2 0.5359307861282367 1106 3 0.35676605193905075 1106 4 0.15409659572339507 1106 5 0.28205820109990043 1106 6 -0.982856127984018 1106 7 -0.7932802598053428 1106 8 -0.7065733322853909 1106 9 -0.6055193195401429 1106 10 -0.6132530384455137 1106 11 -0.5415838652283753 1106 12 -0.4240838064229209 1106 13 -0.5456349975849264 1106 14 -0.48983396292679515 1106 15 -0.67414408303123 1106 16 -0.6772003341716015 1106 17 -0.6387851023642739 1106 18 -0.6476008685437691 1106 19 -0.4730016268708752 1106 20 -0.5761826905829324 1106 21 -0.6035949465328585 1106 22 -0.7340560473209976 1106 23 -0.4649802831362493 1106 24 -0.4836676198145682 1106 25 -0.41892754922717756 1107 1 1.0785122378651728 1107 2 0.9598288644310955 1107 3 1.0065564621984429 1107 4 0.8592448760209876 1107 5 0.9897758544704658 1107 6 0.6069054859054646 1107 7 0.7164461908400739 1107 8 0.82476493941531 1107 9 1.1553617330301975 1107 10 0.612469617930735 1107 11 0.9857056239350115 1107 12 0.7730969934116061 1107 13 0.835918519709054 1107 14 1.0344102535598552 1107 15 1.0946761707388006 1107 16 0.9603176837590652 1107 17 0.9393666619385712 1107 18 0.9898234238282626 1107 19 1.2500587388527151 1107 20 1.0370749512820154 1107 21 1.1974790190924836 1107 22 1.1819908994881345 1107 23 1.3862352404017884 1107 24 1.290301075848845 1107 25 1.1948407353365482 1108 1 0.9804779444518488 1108 2 0.9837058758961277 1108 3 0.6776390307725606 1108 4 0.47973894013365737 1108 5 -0.4959087427280977 1108 6 0.1474589088914584 1108 7 0.5728854210813168 1108 8 0.704985851025242 1108 9 1.0244322010374205 1108 10 1.0739657920162031 1108 11 0.9632108988670041 1108 12 0.8585699577962771 1108 13 0.7728608386454283 1108 14 1.3094312261810472 1108 15 0.9180517458970456 1108 16 0.9663679672072192 1108 17 0.9237674805063278 1108 18 1.0816390172898878 1108 19 0.9953557874329348 1108 20 0.9442170821003302 1108 21 1.0376676455764553 1108 22 1.0643186062273315 1108 23 1.3685165531607737 1108 24 1.0186198342696713 1108 25 1.0539555636152684 1109 1 0.79923119342698 1109 2 -0.2819352661638703 1109 3 0.6213009794338191 1109 4 0.874646521185305 1109 5 -0.460679981977691 1109 6 0.5122850928344462 1109 7 0.8406272928858058 1109 8 1.0914510318821158 1109 9 1.4548991980302688 1109 10 -0.6224335210106275 1109 11 0.5566382632983817 1109 12 1.0287438725563334 1109 13 0.8009319837981224 1109 14 1.3113049389288776 1109 15 1.212445084099734 1109 16 1.4111436151926924 1109 17 1.2832686353319218 1109 18 1.3438485835887235 1109 19 1.2743744817890956 1109 20 1.4850439503844086 1109 21 1.554669244565737 1109 22 1.4504169407780054 1109 23 1.585778399170863 1109 24 1.6545766944601041 1109 25 1.8370342431001703 1110 1 0.46109653187575006 1110 2 -0.580159534099387 1110 3 0.05025616416069312 1110 4 0.5565490345477357 1110 5 0.8375624074338432 1110 6 0.3291986430387013 1110 7 0.4168565761555456 1110 8 0.6018950425617078 1110 9 1.0500792305798896 1110 10 0.5842652120393197 1110 11 0.4887785131676452 1110 12 0.6639715041829675 1110 13 0.5357138271146624 1110 14 0.6933220490539469 1110 15 0.7517652353536519 1110 16 0.16272665556216026 1110 17 0.5528385350424969 1110 18 0.5087824919768948 1110 19 0.5135287783910556 1110 20 0.5007820208441558 1110 21 0.47981978225581656 1110 22 0.5448160240039002 1110 23 0.6580563377407884 1110 24 0.6425681963507728 1110 25 0.8066065590075482 1111 1 0.35323242603221416 1111 2 0.40926057686667505 1111 3 0.4660118238680724 1111 4 0.17996715635907634 1111 5 0.46059857185409925 1111 6 0.27359407972264904 1111 7 0.42034462427886865 1111 8 0.4131543527731125 1111 9 0.6952854586349062 1111 10 0.7042949088131039 1111 11 0.4051000816070527 1111 12 0.4803583030079963 1111 13 0.38630069389834176 1111 14 0.9618372682371401 1111 15 0.7197676230007204 1111 16 0.5740393736685121 1111 17 0.48499893399781513 1111 18 0.7640614820881666 1111 19 0.548100056021994 1111 20 0.6329698678197886 1111 21 0.7038501933395267 1111 22 0.5584027940581109 1111 23 0.9209132963513227 1111 24 0.47242023333284777 1111 25 0.6068684726471432 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13 -0.14003952405423092 1113 14 0.4287842727580937 1113 15 -0.008309712509273481 1113 16 0.04487892994625042 1113 17 0.14106111374256614 1113 18 -0.04792767840609713 1113 19 -2.3442680659923596 1113 20 -0.7509750619651783 1113 21 -0.476220548165891 1113 22 -0.3565422966178744 1113 23 0.03246046984995843 1113 24 -0.26528023813679624 1113 25 0.047132839330409126 1114 1 0.7326838520301528 1114 2 -0.7804798594635756 1114 3 -0.4713352050502806 1114 4 0.2505681280989407 1114 5 0.5573092064296787 1114 6 0.3876661201165419 1114 7 0.5336250690966864 1114 8 0.5161423229453774 1114 9 0.4131968241805808 1114 10 0.05208511000309812 1114 11 0.5464847676103588 1114 12 0.41280852515708233 1114 13 -0.23800059742441704 1114 14 0.002053614599176967 1114 15 0.03268518100631468 1114 16 -0.13315860317255357 1114 17 -3.350343123631035 1114 18 -1.042054762942712 1114 19 -0.43942928238078593 1114 20 -0.12936005605192236 1114 21 0.14875721983286638 1114 22 0.07319541160659071 1114 23 -2.220525952585568 1114 24 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2202 17 0.3034893217457153 2202 18 0.20771412083089302 2202 19 0.21714128696336843 2202 20 0.3426962286387152 2202 21 0.5629583373587153 2202 22 0.6160029632596746 2202 23 0.6812542920820519 2202 24 0.7140032231176434 2202 25 0.6510459432543456 2203 1 -0.15153898127512022 2203 2 -0.13352997450931525 2203 3 -0.12346495419445347 2203 4 -0.5025830027164844 2203 5 -0.4687584741360098 2203 6 -0.56848368252858 2203 7 -0.25071187461507805 2203 8 -0.16263127078416062 2203 9 -0.05496076042012987 2203 10 -0.09276215041181501 2203 11 -0.31716826192739944 2203 12 -0.3240079815679522 2203 13 -0.3832425676060609 2203 14 0.05156069505636651 2203 15 -0.03771906913866019 2203 16 -0.1444424387787393 2203 17 -0.14952003217435106 2203 18 -0.25821167410494894 2203 19 -0.12017455428156154 2203 20 0.07476849586831236 2203 21 -0.10401042519023918 2203 22 -0.06323199733891727 2203 23 0.24732671404368745 2203 24 0.11073432462554678 2203 25 -0.24910584150602233 2204 1 0.2530399287318694 2204 2 0.38564444465389514 2204 3 0.3429231029578581 2204 4 0.04121259824210455 2204 5 0.24370262994759173 2204 6 0.11173984556454997 2204 7 0.21454466496935254 2204 8 0.388943513453583 2204 9 0.6724048852120532 2204 10 0.7007892669206868 2204 11 0.5547941204508149 2204 12 0.6534337212592405 2204 13 0.7319507244162082 2204 14 0.9781134627376673 2204 15 0.7763117487030229 2204 16 0.7545642809411532 2204 17 0.7094539162348568 2204 18 0.6178417847627813 2204 19 0.7479529442640072 2204 20 0.7426307282670734 2204 21 0.5799946132653488 2204 22 0.670677235737692 2204 23 0.7820648371122374 2204 24 0.7292554525137191 2204 25 0.8020419549702567 2205 1 0.24322414546913507 2205 2 0.45812869989904215 2205 3 0.37368634573556037 2205 4 0.060599039327115634 2205 5 -0.11701270571277433 2205 6 -0.3439591138945204 2205 7 -0.09608889320031722 2205 8 -0.1358973827449006 2205 9 0.16470537918950612 2205 10 0.21364634471074595 2205 11 0.04344588912196201 2205 12 0.2391178986605027 2205 13 0.016181125767708693 2205 14 0.3891623263410935 2205 15 0.2524422448750059 2205 16 0.4207009466554723 2205 17 0.4492781049814454 2205 18 0.35003892855452534 2205 19 0.36369231629569754 2205 20 0.3503778515626834 2205 21 0.42906110768170436 2205 22 0.08631309195837389 2205 23 0.3259584394210506 2205 24 0.21022800426127475 2205 25 0.14069517286275612 2206 1 0.09902982018268247 2206 2 0.6386637350517752 2206 3 0.6017647440359042 2206 4 0.596116680566503 2206 5 0.7620529694873142 2206 6 0.41832325946238313 2206 7 0.5428053566719626 2206 8 0.5980714298468328 2206 9 1.1093976001773527 2206 10 0.881344752904134 2206 11 0.610695866822077 2206 12 0.6399807016348973 2206 13 0.4706512398620504 2206 14 0.881519546234216 2206 15 0.7132852617661598 2206 16 0.6143369638142246 2206 17 0.7512088699081758 2206 18 0.6071104260528841 2206 19 0.550779464261538 2206 20 0.7462381812766807 2206 21 0.6057719146133209 2206 22 0.7004009454116087 2206 23 1.0312779765564457 2206 24 0.8215514823952148 2206 25 0.8437960737782717 2207 1 -0.993164549145586 2207 2 -0.0754295343544662 2207 3 0.21460048569524304 2207 4 0.2365483543392557 2207 5 0.25874324936189197 2207 6 0.48571546544476973 2207 7 0.38723689772410097 2207 8 0.32141532552843444 2207 9 0.6094291213213978 2207 10 0.35746499777364704 2207 11 0.11892620343471094 2207 12 0.22004108951087176 2207 13 -0.013880173446509048 2207 14 -5.076925631860249 2207 15 -2.1371286606254642 2207 16 -0.9275115024332621 2207 17 -0.6995103098784696 2207 18 -0.2331387568858787 2207 19 0.03800012885997717 2207 20 0.2748975372499345 2207 21 0.4094947276265728 2207 22 0.1345831376242259 2207 23 0.1769355242114372 2207 24 -0.03685719753244854 2207 25 0.2257858874717229 2208 1 0.26994590316001066 2208 2 0.5865952242689865 2208 3 0.43611805310039387 2208 4 0.3287722235752639 2208 5 0.5201149892761592 2208 6 0.28869210900872844 2208 7 0.45204199367378806 2208 8 0.6767095845725493 2208 9 0.7654925803174351 2208 10 0.817214206539326 2208 11 0.6242945780991889 2208 12 0.5510414500733489 2208 13 0.1956747322063066 2208 14 0.536623504792928 2208 15 0.4117075238222725 2208 16 0.28049298456645744 2208 17 0.29051412824500034 2208 18 0.3418261884288114 2208 19 -0.04538595342839358 2208 20 0.012323922410786281 2208 21 0.21787580210276047 2208 22 0.31868233236802723 2208 23 0.47593396177403946 2208 24 0.4640161649129624 2208 25 0.4340587932046631 2209 1 0.04705747602409005 2209 2 0.3229777505266789 2209 3 0.7600900665417356 2209 4 0.8591345553555882 2209 5 0.7396187310985312 2209 6 0.9104076655743683 2209 7 1.0944382210077563 2209 8 1.1590231833777622 2209 9 1.5150104452871602 2209 10 1.1108482384543876 2209 11 0.9842513969819962 2209 12 0.9855879671753414 2209 13 1.1352552584964641 2209 14 1.3898166347745828 2209 15 1.156098036963293 2209 16 1.2540648421661658 2209 17 1.3793154464054211 2209 18 1.24011761226933 2209 19 1.2208605360766498 2209 20 1.235966395747262 2209 21 1.259218475111311 2209 22 1.4321480228600691 2209 23 1.5326140481362742 2209 24 1.3966000515381793 2209 25 1.4396991237187748 2210 1 -0.3423709403185029 2210 2 -0.47754112430414253 2210 3 0.34218334973740383 2210 4 0.5133587607057493 2210 5 0.7178950881901187 2210 6 0.6604424833968068 2210 7 0.7703494659145349 2210 8 0.8957968746740193 2210 9 1.3917723163790825 2210 10 1.3572164717445148 2210 11 1.0740795933473777 2210 12 0.7508283529925279 2210 13 0.7566712546968617 2210 14 1.1317692539675974 2210 15 0.9395204682114837 2210 16 0.9983448988745778 2210 17 0.9300360860534553 2210 18 1.2272680502596813 2210 19 1.086177163997327 2210 20 0.9577844931585986 2210 21 0.945094759353303 2210 22 0.8215469779682891 2210 23 1.153386361003868 2210 24 0.8669852616414246 2210 25 0.7787131186978726 2211 1 -1.1244343768057057 2211 2 -0.25742387543309064 2211 3 0.3188018061102298 2211 4 0.5179939515269396 2211 5 0.540214568919474 2211 6 0.23774324560424276 2211 7 0.6787570897844688 2211 8 0.5471376954161187 2211 9 0.7779277923322923 2211 10 0.6027792393924472 2211 11 0.41998082060010944 2211 12 0.39404434767772756 2211 13 0.31919022010574594 2211 14 0.40386833195695004 2211 15 0.16689260320030996 2211 16 0.22706279234509452 2211 17 0.17140626390185787 2211 18 0.2253710604852941 2211 19 0.19508664176402846 2211 20 0.32150647148640843 2211 21 0.4516879883656628 2211 22 0.30304751214193715 2211 23 0.5693388509712776 2211 24 0.13977831648624317 2211 25 0.2548521654447953 2212 1 1.037547900660961 2212 2 0.06954288782852913 2212 3 0.5316045194654926 2212 4 0.6842438001120286 2212 5 0.4974354297016106 2212 6 0.8532760863343896 2212 7 0.7578059245884674 2212 8 0.7842515873850715 2212 9 0.9467940906336452 2212 10 0.7191450950963585 2212 11 0.47541545128748197 2212 12 0.5730801375045047 2212 13 0.45997440959336444 2212 14 0.588483644896634 2212 15 0.568222259092444 2212 16 0.25520639890858376 2212 17 0.692744119493959 2212 18 0.610364677764295 2212 19 0.5137819576854241 2212 20 0.7564940321688525 2212 21 0.6380476011161988 2212 22 0.8111708058123241 2212 23 1.0182528566178917 2212 24 0.9518766010717385 2212 25 0.6190949511065043 2213 1 0.31106694644940447 2213 2 0.12574002265185413 2213 3 -0.3512359872275965 2213 4 -1.1891818997997312 2213 5 -1.6780287733006674 2213 6 -0.5793799684896399 2213 7 -0.235007693074742 2213 8 -0.08555695980512468 2213 9 -0.6718119512198939 2213 10 -0.4287591545371825 2213 11 -0.403692262144844 2213 12 -0.6076285202213754 2213 13 -0.7087993110420143 2213 14 -7.961419414993703 2213 15 -3.222955930476779 2213 16 -1.896086847788112 2213 17 -0.8904644574919018 2213 18 -5.196956305992895 2213 19 -2.7418336406606016 2213 20 -4.659746275122444 2213 21 -1.764615881789318 2213 22 -1.2486893212255765 2213 23 -4.1138211408255145 2213 24 -1.9728028610643236 2213 25 -1.5959266507545777 2214 1 0.7916759925756907 2214 2 0.5886486657329115 2214 3 -1.620884442220877 2214 4 -1.4707910952020313 2214 5 0.1595514625554273 2214 6 0.17841970310313116 2214 7 0.0885256701137962 2214 8 0.23098282225189593 2214 9 -4.385011682523071 2214 10 -0.7489897859747146 2214 11 -0.07082914824724185 2214 12 -0.22246655574906526 2214 13 -0.2926700380161904 2214 14 -1.4005931722709093 2214 15 -1.1463138943519837 2214 16 -0.3769981778373719 2214 17 -0.8054517690309382 2214 18 -0.09793779576749273 2214 19 -3.301842597221281 2214 20 -0.8984423491633464 2214 21 -2.6811780552480435 2214 22 -4.969927565739685 2214 23 -5.089261505058982 2214 24 -4.023761959437213 2214 25 -1.9768790317069609 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20587.0 3 11.0 4 350.0 5 2375.0 6 3024.0 7 2605.0 8 1877.0 9 1528.0 10 1325.0 11 1297.0 12 1118.0 13 1167.0 14 1160.0 15 1135.0 16 1235.0 17 1266.0 18 1418.0 19 1722.0 20 2227.0 21 2848.0 22 3602.0 23 4479.0 24 5561.0 25 6436.0 26 7304.0 27 8160.0 28 9425.0 29 11585.0 30 14236.0 31 18026.0 32 22971.0 33 29911.0 34 39672.0 35 52775.0 36 70799.0 37 105700.0 38 167960.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.41494977853536 20.191559356184854 9.304698106210719 30.088792759069065 2 12.787268562702806 22.1436979067252 40.77591143347967 24.293122097092322 3 17.632873679137088 26.15877237493657 29.462619758502594 26.745734187423746 4 11.472447483035184 18.608328269604584 38.48364919335596 31.43557505400427 5 12.065735017639405 39.82036118630966 35.814991426835434 12.298912369215499 6 30.433213976127494 39.44327286717507 18.132025140043837 11.991488016653602 7 27.178721451291104 32.28753003869175 22.73697898407073 17.796769525946416 8 24.003034485396537 39.86523314856547 20.195743432984727 15.935988933053261 9 26.241618546858835 15.504592566147139 20.01093354286634 38.24285534412768 10 14.406402279985242 30.405104520528774 34.76834357545454 20.420149624031453 11 33.56777798626725 22.92569752982371 25.447872506209627 18.058651977699398 12 23.34969118329909 25.599028070197754 32.28502095882656 18.766259787676592 13 29.232902459463457 23.249856490219866 25.574794435159813 21.94244661515686 14 19.96234930172754 24.017517281765986 27.78879326806788 28.231340148438594 15 21.795650569916546 33.55669906370853 23.018172450641156 21.629477915733766 16 20.69143725829432 29.078783329940972 29.58270786688378 20.64707154488093 17 20.356138199940528 30.231038216955525 28.73117417791326 20.68164940519069 18 20.432976706909052 28.35354993830066 31.386040683399695 19.82743267139059 19 23.07793833055851 27.45277633338581 27.62705746347808 21.842227872577595 20 23.12171634291239 28.59182790774593 28.459845564757213 19.82661018458446 21 21.87728582423244 27.461852575070445 27.40158632226385 23.259275278433268 22 22.51823488535914 29.879769811902808 27.21104444907351 20.390950853664545 23 21.061544724238164 29.534916263301508 28.29747040752072 21.10606860493961 24 22.731421556623424 27.827036894513054 29.126979331312793 20.314562217550733 25 21.608677054495555 28.72040160476532 28.648368440887488 21.02255289985164 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 200.0 1 200.0 2 205.0 3 210.0 4 210.0 5 210.0 6 364.5 7 519.0 8 519.0 9 519.0 10 882.0 11 1245.0 12 1245.0 13 1245.0 14 2023.5 15 2802.0 16 2802.0 17 2802.0 18 5101.5 19 7401.0 20 7401.0 21 7401.0 22 12195.5 23 16990.0 24 16990.0 25 16990.0 26 25140.5 27 33291.0 28 33291.0 29 33291.0 30 44741.0 31 56191.0 32 56191.0 33 56191.0 34 62913.5 35 69636.0 36 69636.0 37 69636.0 38 73778.5 39 77921.0 40 77921.0 41 77921.0 42 80116.0 43 82311.0 44 82311.0 45 82311.0 46 83290.0 47 84269.0 48 84269.0 49 84269.0 50 78514.5 51 72760.0 52 72760.0 53 72760.0 54 61959.5 55 51159.0 56 51159.0 57 51159.0 58 42540.5 59 33922.0 60 33922.0 61 33922.0 62 27051.5 63 20181.0 64 20181.0 65 20181.0 66 15405.5 67 10630.0 68 10630.0 69 10630.0 70 7629.0 71 4628.0 72 4628.0 73 4628.0 74 3182.5 75 1737.0 76 1737.0 77 1737.0 78 1186.0 79 635.0 80 635.0 81 635.0 82 406.5 83 178.0 84 178.0 85 178.0 86 113.0 87 48.0 88 48.0 89 48.0 90 30.0 91 12.0 92 12.0 93 12.0 94 6.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0890476198048267 2 0.031325680538483676 3 0.034346939067576014 4 0.03546003431513634 5 0.02719132676183101 6 0.3921275543548261 7 0.21514540999273307 8 0.2262763624683364 9 0.23056972985178342 10 0.016060374286227672 11 0.002385204101915001 12 0.003975340169858335 13 0.0 14 0.07457738158654237 15 0.0020671768883263344 16 0.0020671768883263344 17 0.0031802721358866683 18 0.0033392857426810013 19 0.0012721088543546672 20 7.950680339716671E-4 21 1.590136067943334E-4 22 0.0 23 1.590136067943334E-4 24 4.770408203830002E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 628877.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.80660556803532 #Duplication Level Percentage of deduplicated Percentage of total 1 92.48194362156497 75.65633884014645 2 5.674347195359301 9.283981657336916 3 0.8748642261919838 2.147090180330162 4 0.31060837329017604 1.0163926671951404 5 0.16149657532715744 0.6605743319188636 6 0.10605119815722068 0.5205413122599184 7 0.06467157639866318 0.37033934993359513 8 0.04970065558924658 0.3252673542609812 9 0.03621276371838361 0.2666198950234442 >10 0.20000255707843612 3.0786359808356925 >50 0.01956165611499499 1.1328313271961343 >100 0.017800987715261826 3.0656218305071485 >500 0.0013693067471040028 0.8330963746058607 >1k 0.0013693067471040028 1.6426688984497044 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 1785 0.2838392881278851 No Hit GTCCTACAGTGGACATTTCTAAATT 1757 0.2793869071376438 No Hit CTTTAGGACGTGAAATATGGCGAGG 1593 0.2533086756233731 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1525 0.24249575036135843 No Hit GTATCAACGCAGAGTACTTTTTTTT 1330 0.2114880970364634 No Hit GTCCTACAGTGTGCATTTCTCATTT 1184 0.18827211044449074 No Hit CTGTAGGACCTGGAATATGGCGAGA 1091 0.17348384501261774 No Hit CTGAAGGACCTGGAATATGGCGAGA 951 0.15122194006141107 No Hit GGTATCAACGCAGAGTACTTTTTTT 829 0.13182228003250238 No Hit CCCATGTACTCTGCGTTGATACCAC 805 0.12800595346943838 No Hit GTACATGGGAAGCAGTGGTATCAAC 774 0.12307653165881405 No Hit TATCAACGCAGAGTACTTTTTTTTT 751 0.11941921870254436 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.590136067943334E-4 2 0.0 0.0 0.0 0.0 1.590136067943334E-4 3 0.0 0.0 0.0 0.0 1.590136067943334E-4 4 0.0 0.0 0.0 0.0 1.590136067943334E-4 5 0.0 0.0 0.0 0.0 1.590136067943334E-4 6 0.0 0.0 0.0 0.0 1.590136067943334E-4 7 0.0 0.0 0.0 0.0 1.590136067943334E-4 8 0.0 0.0 0.0 0.0 1.590136067943334E-4 9 0.0 0.0 0.0 0.0 1.590136067943334E-4 10 0.0 0.0 0.0 0.0 1.590136067943334E-4 11 0.0 0.0 0.0 0.0 1.590136067943334E-4 12 0.0 0.0 0.0 1.590136067943334E-4 1.590136067943334E-4 13 0.0 0.0 0.0 4.7704082038300017E-4 1.590136067943334E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGG 35 0.002113394 16.348303 1 ATGTCGA 45 6.911157E-4 14.733125 17 TAGACAG 65 3.2196294E-6 14.677428 5 TCTAGAC 40 0.005120072 14.313932 2 TGTCGAG 40 0.005381429 14.206943 18 TAGGACC 430 0.0 13.533954 4 GGCGTGC 50 0.0014942099 13.304244 8 AAAGGCG 75 1.4123543E-5 12.720437 5 TATGTCG 60 4.1916448E-4 12.628393 16 TTAGGAC 625 0.0 12.211619 3 CGCCACC 55 0.0031165795 12.063967 14 AGGACCT 720 0.0 11.925411 5 GAAATGT 200 0.0 11.924455 6 GACGTGG 435 0.0 11.798749 7 AGGACGT 975 0.0 11.741942 5 TAGGACT 130 2.4319888E-9 11.741942 4 TGTACTG 130 2.4319888E-9 11.741942 5 GGCGAGG 460 0.0 11.735236 19 GGACGTG 935 0.0 11.733154 6 CCTACAC 90 7.0977167E-6 11.660401 3 >>END_MODULE