FastQCFastQC Report
Thu 26 May 2016
SRR1926660_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926660_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28902
Sequences flagged as poor quality0
Sequence length25
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC4241.4670265033561691No Hit
CCCATGTACTCTGCGTTGATACCAC4231.4635665351878764No Hit
GAGTACATGGGAAGCAGTGGTATCA1960.6781537609853989No Hit
CATGTACTCTGCGTTGATACCACTG1610.5570548750951491No Hit
GCGTTGATACCACTGCTTCCCATGT1500.5189952252439277No Hit
GTATCAACGCAGAGTACATGGGAAG1290.44633589370977783No Hit
ACGCAGAGTACATGGGAAGCAGTGG1280.44287592554148497No Hit
GGTATCAACGCAGAGTACATGGGAA1150.39789633935367796No Hit
ACTCTGCGTTGATACCACTGCTTCC1140.3944363711853851No Hit
GNACATGGGAAGCAGTGGTATCAAC1090.3771365303439208No Hit
CNCATGTACTCTGCGTTGATACCAC990.3425368486609923No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA870.3010172306414781No Hit
GTATCAACGCAGAGTACTTTTTTTT850.2940972943048924No Hit
TATCAACGCAGAGTACATGGGAAGC840.29063732613659954No Hit
CAGTGGTATCAACGCAGAGTACATG690.23873780361220676No Hit
GGTATCAACGCAGAGTACTTTTTTT600.2075980900975711No Hit
GTGGTATCAACGCAGAGTACATGGG570.19721818559269255No Hit
GCTTCCCATGTACTCTGCGTTGATA540.18683828108781397No Hit
ATACCACTGCTTCCCATGTACTCTG520.17991834475122828No Hit
GCAGAGTACATGGGAAGCAGTGGTA510.17645837658293542No Hit
CCACTGCTTCCCATGTACTCTGCGT500.1729984084146426No Hit
GCAGTGGTATCAACGCAGAGTACAT470.16261850390976404No Hit
GATACCACTGCTTCCCATGTACTCT470.16261850390976404No Hit
CTGCTTCCCATGTACTCTGCGTTGA450.15569856757317835No Hit
GGGAAGCAGTGGTATCAACGCAGAG450.15569856757317835No Hit
CATGGGAAGCAGTGGTATCAACGCA440.1522385994048855No Hit
AAGCAGTGGTATCAACGCAGAGTAC440.1522385994048855No Hit
TATCAACGCAGAGTACTTTTTTTTT430.14877863123659263No Hit
GTACTCTGCGTTGATACCACTGCTT410.1418586949000069No Hit
CCCNTGTACTCTGCGTTGATACCAC400.13839872673171405No Hit
CCATGTACTCTGCGTTGATACCACT380.13147879039512836No Hit
GNGTACATGGGAAGCAGTGGTATCA350.12109888589024981No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA340.11763891772195696No Hit
ACATGGGAAGCAGTGGTATCAACGC330.1141789495536641No Hit
GNATCAACGCAGAGTACATGGGAAG320.11071898138537124No Hit
ANTCTGCGTTGATACCACTGCTTCC300.10379904504878555No Hit
TACCACTGCTTCCCATGTACTCTGC300.10379904504878555No Hit
CTTCCCATGTACTCTGCGTTGATAC300.10379904504878555No Hit
ANGCAGAGTACATGGGAAGCAGTGG300.10379904504878555No Hit
GNGTTGATACCACTGCTTCCCATGT290.10033907688049269No Hit
GTTGATACCACTGCTTCCCATGTAC290.10033907688049269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGTA2058.20346E-810.0743322
CCCATGT2101.6217928E-79.6206741
ATGTACT2251.6778358E-88.5881194
CATGTAC2301.7233106E-88.5711463
CATGGGA2851.70985E-108.2867814
GTACTCT2656.7666406E-108.2617026
TGTACTC2409.1749826E-98.2535065
TGGGAAG2451.3267709E-88.0850676
TTGATAC2801.8189894E-127.905684516
TGATACC2850.07.905217617
CAGTGGT2202.5249392E-87.81960513
TACTCTG2358.465213E-87.81637057
CGTTGAT2751.8189894E-127.81311114
TACATGG3352.1907908E-87.80886032
ATGGGAA2708.749339E-97.7225785
GATACCA2657.6397555E-117.686592618
GTTGATA2901.8189894E-127.633074815
AGTGGTA2454.9294613E-107.47058614
ATACCAC2854.7293724E-117.44498219
GTACATG3106.608043E-77.3861951
Produced by FastQC (version 0.11.5)