FastQCFastQC Report
Thu 26 May 2016
SRR1926660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926660_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28902
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC13344.615597536502665No Hit
GTACATGGGAAGCAGTGGTATCAAC11664.034322884229465No Hit
GAGTACATGGGAAGCAGTGGTATCA4981.7230641478098403No Hit
CATGTACTCTGCGTTGATACCACTG4451.539685834890319No Hit
GTATCAACGCAGAGTACTTTTTTTT3661.2663483495951837No Hit
ACGCAGAGTACATGGGAAGCAGTGG3341.1556293682098124No Hit
GTATCAACGCAGAGTACATGGGAAG3201.1071898138537124No Hit
GCGTTGATACCACTGCTTCCCATGT3151.0898899730122484No Hit
GGTATCAACGCAGAGTACTTTTTTT2901.003390768804927No Hit
ACTCTGCGTTGATACCACTGCTTCC2790.9653311189537056No Hit
TATCAACGCAGAGTACATGGGAAGC2580.8926717874195557No Hit
GGTATCAACGCAGAGTACATGGGAA2210.7646529651927202No Hit
TATCAACGCAGAGTACTTTTTTTTT1830.6331741747975919No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1780.6158743339561277No Hit
ATACCACTGCTTCCCATGTACTCTG1490.5155352570756349No Hit
CAGTGGTATCAACGCAGAGTACATG1460.5051553525707564No Hit
GCTTCCCATGTACTCTGCGTTGATA1380.4774756072244135No Hit
GATACCACTGCTTCCCATGTACTCT1320.4567157982146564No Hit
GTGGTATCAACGCAGAGTACATGGG1290.44633589370977783No Hit
CTGCTTCCCATGTACTCTGCGTTGA1290.44633589370977783No Hit
GTACTCTGCGTTGATACCACTGCTT1260.43595598920489936No Hit
CCACTGCTTCCCATGTACTCTGCGT1220.4221161165317279No Hit
GCAGAGTACATGGGAAGCAGTGGTA1200.4151961801951422No Hit
CATGGGAAGCAGTGGTATCAACGCA1190.41173621202684935No Hit
GGGAAGCAGTGGTATCAACGCAGAG1170.4048162756902636No Hit
GCAGTGGTATCAACGCAGAGTACAT1150.39789633935367796No Hit
TACCACTGCTTCCCATGTACTCTGC1150.39789633935367796No Hit
ACATGGGAAGCAGTGGTATCAACGC1120.3875164348487994No Hit
AAGCAGTGGTATCAACGCAGAGTAC1100.38059649851221367No Hit
GAGTACTTTTTTTTTTTTTTTTTTT980.33907688049269946No Hit
CCATGTACTCTGCGTTGATACCACT930.3217770396512352No Hit
CTTCCCATGTACTCTGCGTTGATAC880.304477198809771No Hit
GTTGATACCACTGCTTCCCATGTAC870.3010172306414781No Hit
ATGGGAAGCAGTGGTATCAACGCAG750.25949761262196386No Hit
GTACTTTTTTTTTTTTTTTTTTTTT720.24911770811708533No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA700.24219777178049962No Hit
ATCAACGCAGAGTACATGGGAAGCA680.23527783544391392No Hit
GCAGAGTACTTTTTTTTTTTTTTTT520.17991834475122828No Hit
ATCAACGCAGAGTACTTTTTTTTTT520.17991834475122828No Hit
GTACTGGTTCACTATCGGTCAGTCA470.16261850390976404No Hit
GTACATGGTAAGCAGTGGTATCAAC450.15569856757317835No Hit
AAAAAGTACTCTGCGTTGATACCAC420.14531866306829977No Hit
GTGGTATCAACGCAGAGTACTTTTT390.13493875856342122No Hit
ACCATGTACTCTGCGTTGATACCAC380.13147879039512836No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA350.12109888589024981No Hit
CTGCGTTGATACCACTGCTTCCCAT340.11763891772195696No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA330.1141789495536641No Hit
GGAAGCAGTGGTATCAACGCAGAGT330.1141789495536641No Hit
CTATCGGTCAGTCAGGAGTATTTAG330.1141789495536641No Hit
TCCATGTACTCTGCGTTGATACCAC320.11071898138537124No Hit
GTACATGGAAAGCAGTGGTATCAAC320.11071898138537124No Hit
GGATACCACGTGTCCCGCCCTACTC310.10725901321707841No Hit
TGATACCACTGCTTCCCATGTACTC310.10725901321707841No Hit
CCCATATTCAGACAGGATACCACGT290.10033907688049269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG3000.08.84325212
CTGCGTT3050.08.69828111
TGATACC3250.08.45453817
GAAGCAG3650.08.3356169
TTGATAC3300.08.32643916
GGAAGCA3600.08.1872838
GTTGATA3400.08.08154415
CTCTGCG3300.08.0672359
TCTGCGT3300.08.0393210
CGTTGAT3300.08.0393214
GATACCA3450.07.964420318
CAGTGGT3350.07.91933113
CCATGTA2904.5474735E-117.8959512
AGTGGTA3400.07.8028714
GCGTTGA3400.07.8028713
GTGGTAT3400.07.8028715
ATACCAC3550.07.740070319
TGGTATC3450.07.68978516
ACTCTGC3351.8189894E-127.6630138
GGGAAGC3950.07.46182787
Produced by FastQC (version 0.11.5)