FastQCFastQC Report
Thu 26 May 2016
SRR1926659_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926659_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668114
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9490.14204162762642303No Hit
GTCCTAAAGTGTGTATTTCTCATTT7260.10866409026004545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAC250.00727105318.39235310
TCGAGAC300.007606831418.2481983
GTATAAT858.526695E-817.2499351
GTTAGGC400.00204859416.4233783
GTATTTG500.002442558815.9953931
TAGGACC2150.014.9716554
GATAATA550.004607146614.5412671
TGTAGGA4900.014.2140342
GTCCTAC5850.013.8991321
TCCTACA6150.013.7676512
ACCTACA600.006984460613.6566212
GTCCTAA4050.013.4940571
CCTCTAT600.00819970113.3294941
GTATTAA904.7955262E-513.32949351
TTTAGGA3900.013.3064512
CTAGGAC500.00902293413.1387023
CTGTAGG5100.013.0681321
TGTAGAA2500.012.5640922
GTAGACC801.00548954E-412.31753353
CCTACAG6550.012.2026113
Produced by FastQC (version 0.11.5)