##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926659_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 668114 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.360592653349578 27.0 7.0 31.0 2.0 31.0 2 16.457164495879447 18.0 2.0 30.0 2.0 31.0 3 20.019628386772318 24.0 15.0 31.0 2.0 31.0 4 22.320048674328035 28.0 2.0 35.0 2.0 37.0 5 22.922047734368686 30.0 2.0 35.0 2.0 37.0 6 23.64377636151914 31.0 2.0 35.0 2.0 37.0 7 23.35738960716285 30.0 7.0 35.0 2.0 37.0 8 23.500621151480136 31.0 8.0 35.0 2.0 37.0 9 26.249647515244405 32.0 17.0 37.0 2.0 39.0 10 26.960018799186965 32.0 23.0 37.0 2.0 39.0 11 24.041214523269982 30.0 7.0 37.0 2.0 39.0 12 25.190515391085953 31.0 11.0 37.0 2.0 39.0 13 21.828289782881363 27.0 2.0 35.0 2.0 39.0 14 25.189538012973834 31.0 17.0 38.0 2.0 40.0 15 26.292385730578914 31.0 17.0 38.0 2.0 40.0 16 26.67123125694118 31.0 17.0 39.0 2.0 40.0 17 26.000353233130873 31.0 11.0 38.0 2.0 40.0 18 26.072522054619423 31.0 16.0 38.0 2.0 40.0 19 26.451355606977252 31.0 18.0 39.0 2.0 40.0 20 26.708630563047624 31.0 18.0 39.0 2.0 40.0 21 24.554632891991485 30.0 2.0 38.0 2.0 40.0 22 25.493743582681997 31.0 2.0 38.0 2.0 40.0 23 25.48949430785764 31.0 2.0 38.0 2.0 40.0 24 24.555104368416167 31.0 2.0 39.0 2.0 40.0 25 24.354119207201165 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.830673550519887 1101 2 10.438375111437468 1101 3 7.132386162355015 1101 4 9.031918233710272 1101 5 8.748617743960704 1101 6 8.350512731121555 1101 7 7.955287337863513 1101 8 6.063020552324275 1101 9 6.440195097054868 1101 10 5.901441522397388 1101 11 8.660841291706951 1101 12 6.159207008071533 1101 13 10.805340460317954 1101 14 8.15074863701906 1101 15 7.4386518552681125 1101 16 6.849164449195197 1101 17 3.0323644615532253 1101 18 5.5328796901504695 1101 19 5.933894289991663 1101 20 3.8003840518280896 1101 21 1.5964726665683742 1101 22 5.05729816307851 1101 23 6.330862353288207 1101 24 7.737585347289702 1101 25 7.9959902202303255 1102 1 5.405531695211572 1102 2 10.384623372459997 1102 3 6.719174458908885 1102 4 7.8826019273933134 1102 5 7.763399599911327 1102 6 6.379053858577475 1102 7 6.403631130608055 1102 8 6.500926100848499 1102 9 5.4791299450686 1102 10 4.7735943801596115 1102 11 7.350415747678888 1102 12 4.8532418591508275 1102 13 9.291818405047302 1102 14 6.833296383172872 1102 15 4.570187892428585 1102 16 4.9692068123652255 1102 17 5.8411543084064235 1102 18 5.7644990814463135 1102 19 4.850586138074007 1102 20 5.0700733642513605 1102 21 4.350040763685431 1102 22 5.561119899092606 1102 23 5.785797837771344 1102 24 7.221332750684304 1102 25 6.9941881067431595 1103 1 4.883144190591228 1103 2 9.156359095696354 1103 3 6.01258196059953 1103 4 7.724522836236517 1103 5 7.252258028061302 1103 6 7.121844795951056 1103 7 6.757359244225601 1103 8 6.062338517748742 1103 9 5.029422421291386 1103 10 4.627761568630881 1103 11 5.624273223298278 1103 12 4.254138072784844 1103 13 8.16866948315253 1103 14 6.519721797751373 1103 15 5.689014374752123 1103 16 5.3651741546075655 1103 17 3.125189819960884 1103 18 2.0336379254916324 1103 19 4.045127037308415 1103 20 4.570690801405213 1103 21 6.584784525369109 1103 22 2.406431209615299 1103 23 4.820415303766762 1103 24 6.308507135125648 1103 25 2.2368306618628004 1104 1 5.886465714063327 1104 2 10.6368811379156 1104 3 7.1702413408236545 1104 4 7.6280293674323865 1104 5 7.812671967728225 1104 6 7.531962632569886 1104 7 7.27993804633082 1104 8 7.260981492140893 1104 9 6.002832095502459 1104 10 4.977210021911894 1104 11 8.057777759312813 1104 12 5.034374894763278 1104 13 9.51943596531531 1104 14 7.007046350271498 1104 15 6.473147846337412 1104 16 6.033486593958589 1104 17 6.412997750504392 1104 18 4.13387916105302 1104 19 4.8621681531589225 1104 20 3.855168895221059 1104 21 3.164345404095336 1104 22 5.455143108453559 1104 23 5.911776470887386 1104 24 6.798661217538729 1104 25 6.114321993404502 1105 1 3.369043379890364 1105 2 9.185212193070443 1105 3 5.79937752431589 1105 4 5.451767844004401 1105 5 2.4424815750504933 1105 6 4.739594243944158 1105 7 5.678486029927932 1105 8 2.82281326227125 1105 9 3.591756995850062 1105 10 3.175324148624469 1105 11 6.346163634033058 1105 12 3.8146037442401557 1105 13 4.919262021372827 1105 14 3.1722114250788565 1105 15 4.018291600832878 1105 16 3.9782851829207395 1105 17 4.776807094188392 1105 18 1.941648179544952 1105 19 1.7605406167152324 1105 20 0.8971375279987441 1105 21 2.28736417685921 1105 22 3.5398075284637045 1105 23 1.235088684503154 1105 24 4.296411695383085 1105 25 4.812681131765878 1106 1 6.423850843553719 1106 2 11.077920454067185 1106 3 7.683169708386476 1106 4 9.184060372134127 1106 5 8.56831462955914 1106 6 7.58728652249949 1106 7 7.355335584544953 1106 8 7.162337555792629 1106 9 6.436250681786142 1106 10 5.430089946410284 1106 11 7.691409620535836 1106 12 5.100299090919197 1106 13 4.743453061525688 1106 14 5.579169205760621 1106 15 5.720241690161995 1106 16 5.69102420932807 1106 17 5.938232908935166 1106 18 6.195142957632065 1106 19 5.981283489851055 1106 20 5.460876851117625 1106 21 5.864819570170365 1106 22 6.299441710476252 1106 23 6.69046095228428 1106 24 7.513541421461504 1106 25 6.459335551189749 1107 1 4.0484315260291375 1107 2 -13.886156404746517 1107 3 -5.51656038608046 1107 4 1.9227596845176507 1107 5 4.688910992260766 1107 6 4.764460225988984 1107 7 4.733903799955275 1107 8 5.434832896709022 1107 9 3.5152558871071378 1107 10 2.9549469607409833 1107 11 5.737267855867756 1107 12 3.2398852357684973 1107 13 4.333408719902398 1107 14 3.883365594167536 1107 15 3.7889583536154987 1107 16 3.3645927460545018 1107 17 3.735957990870773 1107 18 -1.2485367544686987 1107 19 1.6098549184224815 1107 20 1.9015940286769073 1107 21 -1.356438703719487 1107 22 1.7654593185991203 1107 23 -1.0617336367524146 1107 24 2.3531082873441598 1107 25 3.2108360974271406 1108 1 5.204459080564156 1108 2 10.504390858935077 1108 3 0.49983201090895335 1108 4 5.9073112854871646 1108 5 7.428431339243801 1108 6 7.641931842266569 1108 7 7.765527731579208 1108 8 7.490745640681467 1108 9 6.059126278738724 1108 10 4.962128999266305 1108 11 7.3156069297292206 1108 12 3.15245061400552 1108 13 3.4697526413209445 1108 14 5.178696631289615 1108 15 5.403934753666526 1108 16 5.345508679209239 1108 17 0.49369494711519124 1108 18 4.038732029815012 1108 19 5.175194885254985 1108 20 5.523994845107577 1108 21 5.213595739449286 1108 22 1.8297787470976559 1108 23 5.014691927732851 1108 24 5.716611285175514 1108 25 6.049093528221885 1109 1 3.705492262735895 1109 2 -13.886156404746517 1109 3 -5.67110240256938 1109 4 2.1488469698969723 1109 5 2.0588603153074345 1109 6 2.8412244647001224 1109 7 3.873688990182945 1109 8 3.9074673419098396 1109 9 3.2995157766767633 1109 10 2.51968487769129 1109 11 1.0144783543171947 1109 12 1.439143990779467 1109 13 2.3551090451425445 1109 14 3.2417046002941525 1109 15 3.4178063983927487 1109 16 3.2753256709467173 1109 17 3.8122305874973534 1109 18 3.8701985908153205 1109 19 2.057445872208124 1109 20 1.2663773279734833 1109 21 0.6380927235553031 1109 22 2.9838769874592685 1109 23 3.316074395922872 1109 24 4.448010337113466 1109 25 4.582774679985192 1110 1 3.0997203072391812 1110 2 9.114718485892153 1110 3 6.550738893817243 1110 4 7.623742254004156 1110 5 8.24740079641986 1110 6 4.372576519144648 1110 7 6.647417495358734 1110 8 7.4691084199609215 1110 9 5.5699971213759305 1110 10 5.082028190971819 1110 11 3.018888422527695 1110 12 5.668655677924768 1110 13 10.098217926443887 1110 14 7.480148688588425 1110 15 6.4733449206792315 1110 16 6.269458483991105 1110 17 6.651860810710598 1110 18 7.08559744029542 1110 19 5.971534708448726 1110 20 6.201277722061388 1110 21 3.4363468134754775 1110 22 5.948156673737422 1110 23 6.5066155119116935 1110 24 7.350449965788201 1110 25 7.619012959934711 1111 1 5.316081419994823 1111 2 9.857369075621067 1111 3 7.699878146147547 1111 4 8.1648456687089 1111 5 8.833978789960142 1111 6 8.131146171622298 1111 7 7.926764155973526 1111 8 7.980215584196973 1111 9 6.565741074434435 1111 10 5.928836813578204 1111 11 7.905277623877524 1111 12 7.050173777635987 1111 13 8.863753813405385 1111 14 6.765468286796544 1111 15 6.292505683478616 1111 16 6.153012513421633 1111 17 7.542242933997823 1111 18 7.258635021124132 1111 19 6.169253414663078 1111 20 5.636315447608855 1111 21 8.210684231824501 1111 22 7.626927090593206 1111 23 7.231814335742921 1111 24 7.367342591052147 1111 25 7.806871056619993 1112 1 -2.129861682035063 1112 2 7.308075995592754 1112 3 5.54848822095288 1112 4 -0.8638456524693652 1112 5 3.5898570351915424 1112 6 2.549284392492538 1112 7 5.244747409272168 1112 8 5.520165969064994 1112 9 4.086483133143545 1112 10 2.9362153107116242 1112 11 -0.7679321180345084 1112 12 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3.431239550940539 2204 7 4.462169012579011 2204 8 4.390950241578562 2204 9 2.5099579434963672 2204 10 2.4659514220090664 2204 11 3.9084438581509637 2204 12 4.172864071032045 2204 13 1.9297330555940455 2204 14 3.530763187786569 2204 15 3.586039752981609 2204 16 3.532345438587509 2204 17 -1.7401839886103971 2204 18 1.652989389763949 2204 19 2.4979236549973685 2204 20 2.904725928996857 2204 21 4.047125804414442 2204 22 3.733868433405423 2204 23 2.3053476124026453 2204 24 3.8038916379517644 2204 25 3.8688611225874894 2205 1 6.476072215346473 2205 2 10.71606771843569 2205 3 7.52452959512928 2205 4 8.282650159976988 2205 5 8.189318236380586 2205 6 7.796747300192223 2205 7 7.82417234711005 2205 8 7.6815197427970645 2205 9 6.321312638074069 2205 10 5.453196615278063 2205 11 7.75025433711771 2205 12 7.0738707446043065 2205 13 10.314151264117996 2205 14 7.85900552310088 2205 15 6.595258573688177 2205 16 6.164873503221482 2205 17 6.866201533090365 2205 18 6.846762025261217 2205 19 5.591586692968626 2205 20 6.020674548955032 2205 21 -0.5636067851390649 2205 22 4.148560515472774 2205 23 5.488328875585545 2205 24 7.552771232516086 2205 25 7.837061302694259 2206 1 4.515457310701983 2206 2 8.889473847354322 2206 3 6.140848218386967 2206 4 2.2477686624678057 2206 5 6.125447658209197 2206 6 6.4284335076093235 2206 7 6.581400747452221 2206 8 6.52177536600443 2206 9 5.246678712647611 2206 10 4.498579798491466 2206 11 4.164474636015715 2206 12 5.133514482338921 2206 13 8.557979573885017 2206 14 0.8611290072250668 2206 15 3.2038330214313433 2206 16 4.049832996000632 2206 17 5.15940839667412 2206 18 -0.597348073868254 2206 19 1.5654104739780372 2206 20 3.139322966405846 2206 21 3.958342593414752 2206 22 -1.7218566334227141 2206 23 -2.491533248904968 2206 24 1.9177136166553694 2206 25 3.5359240813386528 2207 1 1.0537070933736707 2207 2 -13.886156404746517 2207 3 -6.3964380754409085 2207 4 -4.48575785158609 2207 5 0.8585322713648047 2207 6 1.4329394479164819 2207 7 2.4996273484374427 2207 8 2.784633957716977 2207 9 0.398707110213536 2207 10 0.17092839310172536 2207 11 -1.854273552915032 2207 12 -3.050381489843055 2207 13 -0.15203926123523814 2207 14 -5.585400984081801 2207 15 -1.7147920147900422 2207 16 -0.6705089338747676 2207 17 0.46832610168716116 2207 18 0.5670173274646935 2207 19 0.0473070257021746 2207 20 -0.1759479695547448 2207 21 -3.5105722138874818 2207 22 -4.248805343941402 2207 23 -1.4865346977603728 2207 24 0.3186771054991837 2207 25 1.0708125194725255 2208 1 4.699572510179831 2208 2 6.213358158360281 2208 3 6.026921482644127 2208 4 7.949467011710414 2208 5 3.608473684153555 2208 6 5.935109964599885 2208 7 6.659001302237243 2208 8 6.241566777714748 2208 9 5.021321773755549 2208 10 4.425858088448226 2208 11 6.302025967227809 2208 12 5.976683011886664 2208 13 8.56463019529863 2208 14 2.9201592212524226 2208 15 4.619632156619399 2208 16 5.080724187428661 2208 17 5.712648721930439 2208 18 0.6481606150442047 2208 19 3.2356370111948642 2208 20 4.259572619665207 2208 21 6.869974042110734 2208 22 5.931487034141401 2208 23 2.5074224498793996 2208 24 3.791785575536011 2208 25 5.1098818197738325 2209 1 2.754344767525204 2209 2 4.5240101158494515 2209 3 5.061872088057534 2209 4 0.4833617954265108 2209 5 4.910914482975912 2209 6 5.388092217719134 2209 7 6.001383218968435 2209 8 4.40322095037315 2209 9 4.1069560751263445 2209 10 3.1575425246862387 2209 11 -1.0057166306750105 2209 12 2.739396835280097 2209 13 2.0627277308165013 2209 14 3.490875875297448 2209 15 3.997007223627559 2209 16 3.279858257639031 2209 17 1.186778608562559 2209 18 3.1009145440623556 2209 19 3.5797838306826826 2209 20 3.5492265597450228 2209 21 5.923837257538068 2209 22 4.372395054982711 2209 23 -0.11811467902303008 2209 24 -3.2737335143442685 2209 25 -0.38981185720854583 2210 1 -2.4332699285984773 2210 2 -13.886156404746517 2210 3 -7.703893361015988 2210 4 -2.1419864373843502 2210 5 -0.8860634913737009 2210 6 -1.537014658895199 2210 7 -1.597685071013725 2210 8 -20.942090180214056 2210 9 -13.30018170882271 2210 10 -7.539008582348561 2210 11 -10.57793054848288 2210 12 -5.281699661659609 2210 13 -5.563923553820031 2210 14 -4.329775139301368 2210 15 -6.143364453730097 2210 16 -5.647659784156097 2210 17 -5.264412872945851 2210 18 -5.1924638594714665 2210 19 -10.651727174153232 2210 20 -7.570626245731152 2210 21 -5.893451068443795 2210 22 -10.362428705460278 2210 23 -8.409331981889505 2210 24 -11.046493579440934 2210 25 -8.553812633766714 2211 1 -0.05021263825919675 2211 2 4.8070951289958135 2211 3 0.7025381704414109 2211 4 1.600802636793734 2211 5 1.1387847721958941 2211 6 0.18388538161045886 2211 7 -20.79002782397635 2211 8 -13.304929794587412 2211 9 -8.899178894198808 2211 10 -7.036147221150745 2211 11 -9.425523817353364 2211 12 -4.674275382073102 2211 13 -6.7514703410502825 2211 14 -4.616924299987389 2211 15 -4.946901628256754 2211 16 -11.907696518872802 2211 17 -7.404807719220088 2211 18 -5.832477217550267 2211 19 -8.73309960490014 2211 20 -6.694419364882499 2211 21 -4.589747317396199 2211 22 -5.923836321003268 2211 23 -7.850805301284204 2211 24 -22.052874618638747 2211 25 -21.84642885983782 2212 1 -1.239610840960598 2212 2 -13.886156404746517 2212 3 -7.864624018354618 2212 4 -19.78920612744816 2212 5 -20.28715738380761 2212 6 -20.97912951760076 2212 7 -10.030219211536158 2212 8 -9.575583003180563 2212 9 -5.350731926249097 2212 10 -5.636698236167373 2212 11 -5.863067481309685 2212 12 -2.9638157416166884 2212 13 -5.850319248036097 2212 14 -3.6684194628806814 2212 15 -4.020018050097747 2212 16 -4.079401855834838 2212 17 -4.247929466700896 2212 18 -3.305441837689571 2212 19 -3.6661684733903854 2212 20 -3.812625085542205 2212 21 -1.8276692275815911 2212 22 -3.175533606604329 2212 23 -3.3240450095901046 2212 24 -2.2882813172033387 2212 25 -2.887577185196669 2213 1 -18.863534285936673 2213 2 -13.886156404746517 2213 3 -8.67851501986608 2213 4 -5.886791640526631 2213 5 -20.28715738380761 2213 6 -10.94995446729895 2213 7 -20.79002782397635 2213 8 -10.766033842185886 2213 9 -8.438124222610998 2213 10 -7.323848891294304 2213 11 -6.626021280660815 2213 12 -5.27569370797946 2213 13 -19.15462546813179 2213 14 -12.418383361102547 2213 15 -7.181431506915207 2213 16 -6.427193871084231 2213 17 -5.116328376908552 2213 18 -5.164882693349849 2213 19 -6.15430783588112 2213 20 -6.073151882688723 2213 21 -4.325844884393273 2213 22 -5.046640631528998 2213 23 -4.877567099064752 2213 24 -3.801365564312789 2213 25 -4.636167290421319 2214 1 -18.863534285936673 2214 2 -13.886156404746517 2214 3 -11.988455145737072 2214 4 -19.78920612744816 2214 5 -20.28715738380761 2214 6 -20.97912951760076 2214 7 -16.351218562675356 2214 8 -14.616842109651762 2214 9 -13.45196581682901 2214 10 -13.309263029929868 2214 11 -14.17160237472243 2214 12 -11.83691439483488 2214 13 -19.15462546813179 2214 14 -16.32328354500641 2214 15 -23.775998911341766 2214 16 -17.862620099901456 2214 17 -23.51874286383008 2214 18 -17.44553352224827 2214 19 -14.474501323155371 2214 20 -13.041130648336633 2214 21 -10.487866628973212 2214 22 -11.566058128794841 2214 23 -11.519209677800674 2214 24 -10.310868003423751 2214 25 -10.098909565460751 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 119757.0 3 1.0 4 615.0 5 2412.0 6 3277.0 7 3090.0 8 2630.0 9 2244.0 10 2234.0 11 2018.0 12 2047.0 13 2123.0 14 3127.0 15 6803.0 16 9980.0 17 10353.0 18 10863.0 19 12351.0 20 14851.0 21 16932.0 22 18003.0 23 19643.0 24 20563.0 25 21381.0 26 17880.0 27 14059.0 28 14860.0 29 17331.0 30 16683.0 31 19237.0 32 24906.0 33 30863.0 34 37957.0 35 43781.0 36 43421.0 37 45216.0 38 34622.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13201388529092 19.247361967428287 12.90831217726197 25.712311970018824 2 13.163838753728307 21.075257191610984 42.50448337215397 23.25642068250674 3 18.25076219512195 25.341241107723576 29.354198424796746 27.05379827235772 4 11.034293667072735 17.274207039208132 40.240656095013 31.450843198706135 5 11.78178249096693 37.93370392962242 37.677961386331944 12.606552193078707 6 30.62477828238658 38.11470659515505 18.863822358970037 12.396692763488334 7 26.362217211388362 32.384303425262864 23.245863997158693 18.007615366190084 8 23.51410807605952 38.66445490567077 20.905839243422225 16.915597774847484 9 26.346543687662095 15.324880025051279 20.669252701777413 37.65932358550921 10 14.182637523587843 29.694094910495338 35.53704317069243 20.58622439522439 11 32.58025838315983 22.10454433565989 26.09463461889675 19.220562662283523 12 23.61757492277888 24.79585312240542 32.69896964279466 18.887602312021045 13 28.191522578089735 22.10884657175941 25.624780321047965 24.07485052910289 14 19.683779071359673 23.241112372235122 28.24809588870192 28.82701266770329 15 21.64491417222301 33.049091144596524 23.762852578534112 21.543142104646357 16 20.559886670864042 28.15312395837191 29.836394948335247 21.450594422428797 17 20.494890306149976 28.94419187597989 29.672760683800213 20.888157134069917 18 20.306359638121037 27.481608844173433 32.33088421971179 19.881147297993735 19 22.823284147251307 26.73568217008325 27.602098727726087 22.83893495493935 20 22.912948656563184 28.161815804838803 28.900739824597476 20.02449571400053 21 21.52316064766137 26.487018410368346 27.04705463878917 24.942766303181113 22 22.246791233073505 29.161505145288587 28.05468219739281 20.5370214242451 23 21.054815969074713 28.61038484301372 29.037405512312432 21.297393675599142 24 22.33306222443207 27.51184592233553 29.186903793658686 20.968188059573713 25 21.84271959292412 27.950805671012922 29.078775056413452 21.127699679649503 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 697.0 1 697.0 2 1434.0 3 2171.0 4 2171.0 5 2171.0 6 3148.5 7 4126.0 8 4126.0 9 4126.0 10 4852.5 11 5579.0 12 5579.0 13 5579.0 14 7107.5 15 8636.0 16 8636.0 17 8636.0 18 12490.0 19 16344.0 20 16344.0 21 16344.0 22 22996.0 23 29648.0 24 29648.0 25 29648.0 26 38885.0 27 48122.0 28 48122.0 29 48122.0 30 57735.5 31 67349.0 32 67349.0 33 67349.0 34 73001.0 35 78653.0 36 78653.0 37 78653.0 38 80393.5 39 82134.0 40 82134.0 41 82134.0 42 81786.5 43 81439.0 44 81439.0 45 81439.0 46 79166.0 47 76893.0 48 76893.0 49 76893.0 50 69812.0 51 62731.0 52 62731.0 53 62731.0 54 53196.5 55 43662.0 56 43662.0 57 43662.0 58 36204.5 59 28747.0 60 28747.0 61 28747.0 62 22717.5 63 16688.0 64 16688.0 65 16688.0 66 12607.5 67 8527.0 68 8527.0 69 8527.0 70 6142.5 71 3758.0 72 3758.0 73 3758.0 74 2644.0 75 1530.0 76 1530.0 77 1530.0 78 1007.0 79 484.0 80 484.0 81 484.0 82 311.5 83 139.0 84 139.0 85 139.0 86 92.0 87 45.0 88 45.0 89 45.0 90 28.5 91 12.0 92 12.0 93 12.0 94 6.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.333652640118304 2 36.31910123122701 3 5.740637076906037 4 13.748551893838476 5 13.381997683030141 6 14.772329273147996 7 9.393456805275747 8 7.604690217537725 9 0.1022280628754973 10 1.4871713509969857 11 5.751862706065133 12 5.8971971849115565 13 16.110573943967648 14 1.6242737017934064 15 2.9345291372430453 16 3.0069718640830754 17 2.9104314533148536 18 1.545394947568828 19 1.4969002296015352 20 0.2806407289773901 21 4.4814208353664196 22 1.6476230104443255 23 0.10462286376277101 24 9.090065467869255 25 0.10776603992731779 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 668114.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.58414034278796 #Duplication Level Percentage of deduplicated Percentage of total 1 96.83733385011462 88.68763973750309 2 2.2392603561665294 4.101614694463937 3 0.41322892355624663 1.1353564718602347 4 0.16275018250800322 0.5962134222250929 5 0.08595958420429527 0.39362673117869384 6 0.05263891225494075 0.2892533716468917 7 0.03160448807546089 0.2026128909955485 8 0.026297063448437148 0.1926715159571894 9 0.02202936802132735 0.1815786659275352 >10 0.11018274656518925 1.8585303779898095 >50 0.010453333630336625 0.6383380448106385 >100 0.007418212736028541 1.179978008996001 >500 8.429787187212547E-4 0.5425860664453346 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 949 0.14204162762642303 No Hit GTCCTAAAGTGTGTATTTCTCATTT 726 0.10866409026004545 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.4967505545460805E-4 0.0 12 0.0 0.0 0.0 1.4967505545460805E-4 0.0 13 0.0 0.0 0.0 1.4967505545460805E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCAC 25 0.007271053 18.392353 10 TCGAGAC 30 0.0076068314 18.248198 3 GTATAAT 85 8.526695E-8 17.249935 1 GTTAGGC 40 0.002048594 16.423378 3 GTATTTG 50 0.0024425588 15.995393 1 TAGGACC 215 0.0 14.971655 4 GATAATA 55 0.0046071466 14.541267 1 TGTAGGA 490 0.0 14.214034 2 GTCCTAC 585 0.0 13.899132 1 TCCTACA 615 0.0 13.767651 2 ACCTACA 60 0.0069844606 13.656621 2 GTCCTAA 405 0.0 13.494057 1 CCTCTAT 60 0.008199701 13.329494 1 GTATTAA 90 4.7955262E-5 13.3294935 1 TTTAGGA 390 0.0 13.306451 2 CTAGGAC 50 0.009022934 13.138702 3 CTGTAGG 510 0.0 13.068132 1 TGTAGAA 250 0.0 12.564092 2 GTAGACC 80 1.00548954E-4 12.3175335 3 CCTACAG 655 0.0 12.202611 3 >>END_MODULE