FastQCFastQC Report
Thu 26 May 2016
SRR1926659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926659_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668114
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18260.27330665126011433No Hit
CTGTAGGACGTGGAATATGGCAAGA17670.2644758229882924No Hit
CTTTAGGACGTGAAATATGGCGAGG17360.25983589626919956No Hit
GTCCTAAAGTGTGTATTTCTCATTT16380.24516774083464796No Hit
GTCCTACAGTGTGCATTTCTCATTT13210.19772074825553723No Hit
GTATCAACGCAGAGTACTTTTTTTT11350.16988118794098012No Hit
CTGTAGGACCTGGAATATGGCGAGA10560.1580568585600661No Hit
CCCATGTACTCTGCGTTGATACCAC9480.14189195257096843No Hit
CTGAAGGACCTGGAATATGGCGAGA9000.13470754990914724No Hit
GTACATGGGAAGCAGTGGTATCAAC8770.13126502363369125No Hit
GGTATCAACGCAGAGTACTTTTTTT7220.108065390038227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4000.014.3128444
AGTGCGC400.00526841214.2526488
TGTGCGC400.005355654314.21741110
CTCGAAC707.4961317E-613.52721618
TCGAACT707.501929E-613.52620419
TCTAGAC1009.411451E-913.3606713
TAAGCCT603.9375987E-412.7225284
CCTCGAA854.0765335E-612.25498317
GGACCTG7600.012.1775546
GTCCTAT959.831092E-712.0529211
TGTCGAG550.003141120312.0515218
GTCGAGG550.003142881412.05061919
AGGACCT8050.011.9718215
CTGTAGG11050.011.8302241
ATAATAT1052.5551526E-711.81555753
TTAACCT657.7223475E-411.7438724
GGCGAGG4850.011.71341319
CTTCTAG907.086601E-611.6623171
TGTAGGA10900.011.64901352
CAGTACT751.9874763E-411.4502754
Produced by FastQC (version 0.11.5)