FastQCFastQC Report
Thu 26 May 2016
SRR1926658_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926658_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643555
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15030.2335464723294823No Hit
GTCCTAAAGTGTGTATTTCTCATTT12140.18863966560744613No Hit
CTGTAGGACGTGGAATATGGCAAGA10890.16921630629860696No Hit
CTTTAGGACGTGAAATATGGCGAGG9350.14528672763011707No Hit
GTCCTACAGTGTGCATTTCTCATTT7590.11793863772327151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGGG305.0552166E-420.230127
ACGGGGA350.001877472916.6395958
AAGTGTC556.202954E-616.5519147
TGTAGGA7450.016.0055222
CTGTAGG7100.015.6412271
GGACCTC400.0030220815.4940046
TAGGACC3150.014.8826394
CCTACAC753.7446007E-614.4633233
GTGCTAA658.943693E-414.2375261
TGTAGAA2000.014.2276352
CTAGAGT450.005380657514.2061554
TAGACTC450.00660391313.7738775
GGACGGG602.0115048E-413.7724476
TTAGGAC5950.013.6733083
GTAGGAC8750.013.63684753
GTCCTAC10300.013.6056251
CAAGACT550.001293210913.5604214
TTTAGGA5600.013.5501312
TCCTAGA700.001299712513.5501312
TCGTCAA700.001299712513.5501312
Produced by FastQC (version 0.11.5)