##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926658_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 643555 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.807317167918825 27.0 10.0 31.0 2.0 31.0 2 16.86803925072449 22.0 2.0 31.0 2.0 31.0 3 20.459882993683525 25.0 15.0 31.0 2.0 31.0 4 22.85406841684083 28.0 7.0 35.0 2.0 37.0 5 23.425320291195003 30.0 10.0 35.0 2.0 37.0 6 24.233064773018622 31.0 10.0 35.0 2.0 37.0 7 23.925265128854566 31.0 10.0 35.0 2.0 37.0 8 23.99868853477947 31.0 12.0 35.0 2.0 37.0 9 26.778214760199205 32.0 17.0 37.0 2.0 39.0 10 27.481270443085673 32.0 25.0 37.0 2.0 39.0 11 24.590118948652407 30.0 10.0 37.0 2.0 39.0 12 25.76830263147672 31.0 17.0 37.0 2.0 39.0 13 22.391871712596437 28.0 2.0 37.0 2.0 39.0 14 25.74281763019478 31.0 17.0 38.0 2.0 40.0 15 26.85547466805479 31.0 17.0 38.0 2.0 40.0 16 27.227407136919144 32.0 19.0 39.0 2.0 40.0 17 26.52814133990102 31.0 17.0 38.0 2.0 40.0 18 26.56994662460862 31.0 17.0 38.0 2.0 40.0 19 26.957831110006136 31.0 18.0 39.0 2.0 40.0 20 27.232244330321418 31.0 22.0 39.0 2.0 40.0 21 25.030519536014793 30.0 10.0 38.0 2.0 40.0 22 26.001483944651195 31.0 11.0 38.0 2.0 40.0 23 25.988285383533654 31.0 2.0 38.0 2.0 40.0 24 25.063860897669972 31.0 2.0 39.0 2.0 40.0 25 24.86719083838988 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.642700737508864 1101 2 10.303156675760306 1101 3 6.943368292217311 1101 4 8.718594451061907 1101 5 8.254110550305217 1101 6 7.852843721357942 1101 7 7.602619186428555 1101 8 6.003523632520576 1101 9 5.826847604895203 1101 10 5.333595617313566 1101 11 8.460178025671937 1101 12 5.656037471667027 1101 13 10.195861349749688 1101 14 7.589092811613263 1101 15 6.673907664865769 1101 16 6.307042375468313 1101 17 2.4779265339980228 1101 18 4.742251704624682 1101 19 5.289920990906552 1101 20 3.5192519126171646 1101 21 0.5034629464388374 1101 22 4.530506301558528 1101 23 5.693774555384856 1101 24 7.022309874207064 1101 25 7.520563229867516 1102 1 6.146138548053276 1102 2 10.970924516116977 1102 3 7.157694293345344 1102 4 8.407862036268579 1102 5 8.371090111447216 1102 6 7.527549851224816 1102 7 7.501373875369367 1102 8 7.350706686979432 1102 9 6.465107571412357 1102 10 5.663674669050824 1102 11 8.41032082014637 1102 12 6.017397018305761 1102 13 10.440066758929717 1102 14 7.8507513761483025 1102 15 5.580764485428176 1102 16 6.154686490455916 1102 17 6.94551353119585 1102 18 6.934272224056368 1102 19 5.812897919974851 1102 20 6.2704314218339015 1102 21 4.942790961908987 1102 22 5.963075080150993 1102 23 6.4438887014380875 1102 24 7.952837452788792 1102 25 7.406721566110452 1103 1 4.804983329948318 1103 2 9.141598408627956 1103 3 6.1372822001429554 1103 4 7.884712741888784 1103 5 7.013323704287394 1103 6 6.828855943980937 1103 7 6.500822155869063 1103 8 6.129169147128781 1103 9 5.244273053639656 1103 10 4.393038678599698 1103 11 5.6229620740932305 1103 12 4.153688546278399 1103 13 8.299667136727667 1103 14 6.306528230827528 1103 15 5.310579346092254 1103 16 5.137386034102889 1103 17 3.0696152676603816 1103 18 1.8737695327044577 1103 19 3.80865227794758 1103 20 4.098025955378752 1103 21 6.459007847276652 1103 22 2.0657988322103904 1103 23 4.147876670590701 1103 24 5.485375148526948 1103 25 1.5000783131355995 1104 1 5.738681438632032 1104 2 10.298526303582129 1104 3 6.8786355591200845 1104 4 7.473129817164029 1104 5 7.6959018085902535 1104 6 7.274995092407604 1104 7 6.9897360735237655 1104 8 6.951765189261959 1104 9 5.736961019753142 1104 10 4.7800873283043614 1104 11 7.8157160490542665 1104 12 4.955969847570309 1104 13 9.101092247026084 1104 14 6.9181194436587 1104 15 6.113669477769545 1104 16 5.649618391166353 1104 17 6.098668780707488 1104 18 3.868288282325203 1104 19 4.345208747332649 1104 20 3.7267350680037588 1104 21 2.837813531141631 1104 22 4.931994754166027 1104 23 5.2089133943022325 1104 24 6.353746698417648 1104 25 5.38755616835126 1105 1 3.4411434803463443 1105 2 9.437452181797184 1105 3 6.050255498618412 1105 4 5.438249445469548 1105 5 1.940680282732302 1105 6 4.7408775049479495 1105 7 5.370387117828937 1105 8 2.737482163020445 1105 9 3.533125387452774 1105 10 3.188655986122537 1105 11 6.361924562710566 1105 12 3.8720961484286214 1105 13 4.597483134142699 1105 14 3.3694148741903334 1105 15 3.814810759304958 1105 16 4.2996704978693 1105 17 4.957878640453611 1105 18 2.294114390678196 1105 19 2.0958904766962227 1105 20 1.5845004141514814 1105 21 2.43528490864022 1105 22 3.6648118451645963 1105 23 1.3114521486625499 1105 24 4.399754022304229 1105 25 4.906117733868204 1106 1 6.355700150472021 1106 2 11.252481583388331 1106 3 7.657977740057362 1106 4 9.134902852595111 1106 5 8.483240527169063 1106 6 7.7479454168584745 1106 7 7.685866190814064 1106 8 7.305575671610324 1106 9 6.4690443313015 1106 10 5.705971217299751 1106 11 8.264755186485498 1106 12 5.411860359197675 1106 13 4.706695632701447 1106 14 5.66506228569731 1106 15 6.114211197446313 1106 16 6.205769886777411 1106 17 6.667578283022507 1106 18 6.61520570856128 1106 19 6.428638660715592 1106 20 5.90784008900447 1106 21 6.288879397283136 1106 22 6.600609723638019 1106 23 6.968076154196972 1106 24 7.766486986676419 1106 25 6.5723174340872745 1107 1 4.025859053727981 1107 2 -13.931191885999425 1107 3 -5.537810371624971 1107 4 1.843097708145475 1107 5 4.54819742595911 1107 6 4.9400530487152885 1107 7 4.874416674620818 1107 8 5.459084785179414 1107 9 3.778596269124975 1107 10 2.9790212770182976 1107 11 5.685022275509404 1107 12 3.0219610152361724 1107 13 4.583792272884239 1107 14 4.157515246525406 1107 15 4.04362647500772 1107 16 3.6335493955274742 1107 17 4.488541655648909 1107 18 -1.0170384104603727 1107 19 1.7679243750013072 1107 20 2.269879434004558 1107 21 -0.7600443706973401 1107 22 2.2507953704158155 1107 23 -1.2215864793042677 1107 24 2.58853221698228 1107 25 3.421692553091617 1108 1 5.20953870822763 1108 2 10.37401903211472 1108 3 0.3832250350051183 1108 4 5.758210612423607 1108 5 7.079126400128665 1108 6 7.150297672897366 1108 7 7.108786972029776 1108 8 6.95889220914345 1108 9 5.324775581618006 1108 10 4.853354364106863 1108 11 6.884642595543415 1108 12 2.264958298125105 1108 13 3.762168193934375 1108 14 5.055155295605989 1108 15 4.790461598351094 1108 16 4.745780183700148 1108 17 0.01503401469482668 1108 18 3.39306015058623 1108 19 4.440380950683689 1108 20 4.501130631027731 1108 21 4.309150408913595 1108 22 1.1334944656241426 1108 23 4.2941847614115645 1108 24 5.028773663133602 1108 25 5.686085770205839 1109 1 3.899811255404458 1109 2 -13.931191885999425 1109 3 -5.568312367266222 1109 4 2.290176815041125 1109 5 2.1584407162365125 1109 6 3.268951366922426 1109 7 3.984208143398913 1109 8 4.11694613260126 1109 9 3.6134191111862073 1109 10 2.738532061801042 1109 11 1.0600572310090328 1109 12 1.379419998857351 1109 13 2.7335589665908593 1109 14 3.48154637252005 1109 15 3.536994752285146 1109 16 3.5519996593403853 1109 17 4.080933952265678 1109 18 4.017926333596037 1109 19 2.5499407237751477 1109 20 1.2201836912955244 1109 21 0.9719203248258133 1109 22 3.043641568528564 1109 23 3.523304239981691 1109 24 4.666774758460882 1109 25 5.080644743212559 1110 1 3.2254609580208466 1110 2 8.929919225111686 1110 3 6.172927268255737 1110 4 7.559423736217376 1110 5 8.405566984633769 1110 6 4.104667675654468 1110 7 6.650901667370277 1110 8 7.462614293510697 1110 9 5.588009659368211 1110 10 5.422350779877473 1110 11 3.314129775030594 1110 12 5.985181623187142 1110 13 10.489008557871514 1110 14 7.740294529132321 1110 15 6.994486963666784 1110 16 6.381360553151481 1110 17 7.015861144554215 1110 18 6.986456897208786 1110 19 5.768820432348974 1110 20 6.2762202367625015 1110 21 4.008075140096391 1110 22 6.170163889390341 1110 23 6.731736457027779 1110 24 7.708398697597346 1110 25 8.245018772687231 1111 1 4.9079078826381455 1111 2 9.451861953365093 1111 3 7.4778865067414415 1111 4 7.961512269530463 1111 5 8.534102575430556 1111 6 7.4433615356449145 1111 7 7.547606738754336 1111 8 7.524919145981535 1111 9 6.108834095397782 1111 10 5.211173077390658 1111 11 7.100506698034515 1111 12 6.598011297949167 1111 13 8.31535454198449 1111 14 6.2449717428605815 1111 15 6.057851583874498 1111 16 5.697050335408761 1111 17 6.945195199162306 1111 18 6.746973905690087 1111 19 5.711437172883038 1111 20 5.16164748133475 1111 21 7.900614691577378 1111 22 7.310976968505958 1111 23 7.287383753671001 1111 24 7.06062436801907 1111 25 7.7645003090024645 1112 1 -2.2048218844288954 1112 2 7.793445795159997 1112 3 5.782996225712491 1112 4 -0.43310587263411193 1112 5 4.072895954557406 1112 6 2.90566502644144 1112 7 5.542546906578632 1112 8 5.9109254202054125 1112 9 4.643789229260683 1112 10 3.381492512774468 1112 11 -0.6300614358155947 1112 12 3.944099341747215 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5.638972599562059 2204 5 0.7720150102150818 2204 6 3.6238691254273228 2204 7 4.981489617061182 2204 8 4.923124373576009 2204 9 3.1566055939428814 2204 10 2.686200625715408 2204 11 4.587615909722164 2204 12 4.486853902775589 2204 13 2.4818903525220755 2204 14 3.6857022932102375 2204 15 3.8124365617939517 2204 16 3.6995907973677014 2204 17 -1.8025306837738775 2204 18 1.7981418251994796 2204 19 2.9275447373826715 2204 20 3.1749224612113345 2204 21 4.673285230367014 2204 22 4.274273537740019 2204 23 3.174693399887307 2204 24 4.968312540021138 2204 25 4.972655222351847 2205 1 6.334748114835431 2205 2 10.622107606386361 2205 3 7.401067107784147 2205 4 7.985937936679505 2205 5 8.056987030846674 2205 6 7.9306779007149615 2205 7 7.8158171559717005 2205 8 7.413845121522268 2205 9 6.376763002526335 2205 10 5.748993248447238 2205 11 8.044780394538265 2205 12 7.343008706861614 2205 13 10.386216679698926 2205 14 7.841207870834921 2205 15 6.7009804229029974 2205 16 6.224132053979311 2205 17 6.9804646050640144 2205 18 6.8336222630003185 2205 19 5.406672259942084 2205 20 5.597932115662854 2205 21 -0.8721715713276588 2205 22 4.006817638275955 2205 23 5.104096044386548 2205 24 7.2955755256453045 2205 25 7.7946749049944195 2206 1 4.269780515895075 2206 2 8.940950366329108 2206 3 6.01751177382328 2206 4 1.7055516455585291 2206 5 5.876952330264558 2206 6 6.030608270314847 2206 7 6.211834325739499 2206 8 6.005247199832112 2206 9 4.625919486105168 2206 10 3.8543285704018757 2206 11 3.637172485327138 2206 12 4.599707767424302 2206 13 7.967628371588702 2206 14 0.2575009546449891 2206 15 2.305264841596607 2206 16 3.007000539919275 2206 17 4.411179491499119 2206 18 -0.9329396715213178 2206 19 0.9127169236888584 2206 20 2.6691220361609815 2206 21 3.4964984737028644 2206 22 -1.96082285108535 2206 23 -2.9557345216398367 2206 24 1.1079932335443452 2206 25 2.9299427026536335 2207 1 1.0597612930013227 2207 2 -13.931191885999425 2207 3 -6.365350265843794 2207 4 -4.59988323051283 2207 5 0.8888117918194993 2207 6 1.4963881674526895 2207 7 2.543808162783577 2207 8 2.7795224792490423 2207 9 0.07734488043681864 2207 10 -0.0461700006332002 2207 11 -1.4067676395123705 2207 12 -3.102574932991491 2207 13 0.1400412601090366 2207 14 -6.194211544316623 2207 15 -2.235165902791369 2207 16 -0.8633433006662443 2207 17 0.029935876662371186 2207 18 -0.0443406400986639 2207 19 -0.5918002377353524 2207 20 -0.5642830976195761 2207 21 -4.353250346180516 2207 22 -4.782914167846286 2207 23 -2.0879848244113433 2207 24 -0.19211271826580045 2207 25 0.4513232532824212 2208 1 4.94276853099818 2208 2 7.0770521370838395 2208 3 6.522538114908045 2208 4 8.287852614527914 2208 5 3.741447396066661 2208 6 6.387767132849078 2208 7 6.550702067456559 2208 8 6.719845542790452 2208 9 4.76434089180534 2208 10 4.415579009303613 2208 11 6.38932545977206 2208 12 6.037147778369796 2208 13 8.626889843939114 2208 14 3.1520849595648777 2208 15 4.585187469241102 2208 16 4.924380909092353 2208 17 5.965705571860546 2208 18 0.68077723807993 2208 19 2.9888641936328 2208 20 4.072512347025569 2208 21 6.838150754630693 2208 22 5.844646126327703 2208 23 1.7071107622693802 2208 24 3.1352913509612925 2208 25 4.304741401200914 2209 1 3.298370575285304 2209 2 4.824052869245332 2209 3 5.2540423896934385 2209 4 1.057462435868981 2209 5 5.44807331343042 2209 6 5.6996246899663205 2209 7 6.038245953908113 2209 8 4.54753609214217 2209 9 4.372955895927351 2209 10 3.3978031948460163 2209 11 -1.1839151907563057 2209 12 2.7225020984108426 2209 13 2.1437288375917944 2209 14 3.3363136533772497 2209 15 3.758739288402978 2209 16 3.428303305739398 2209 17 1.147092816108465 2209 18 3.13802217780632 2209 19 3.5808614379383705 2209 20 3.652006636742449 2209 21 5.768715037833061 2209 22 4.420480329222912 2209 23 0.25525326280264693 2209 24 -3.6330242164062128 2209 25 -0.2546710536154997 2210 1 -2.3984446768578174 2210 2 -13.931191885999425 2210 3 -7.86780661740147 2210 4 -2.2849560745823183 2210 5 -0.8120133667382277 2210 6 -1.6936459737961478 2210 7 -1.596307334333698 2210 8 -21.032009362403283 2210 9 -13.367025889136402 2210 10 -7.4871768069372635 2210 11 -10.256981923659104 2210 12 -4.936068156470888 2210 13 -5.498696360161272 2210 14 -4.408392232722079 2210 15 -6.053635722742527 2210 16 -5.4227848137913455 2210 17 -4.968861812141245 2210 18 -5.221942538715773 2210 19 -9.918778721537855 2210 20 -7.064795686617252 2210 21 -5.026503859844851 2210 22 -10.037633278480861 2210 23 -8.150395278091793 2210 24 -10.852797612409116 2210 25 -8.219799959318175 2211 1 0.033392575756742815 2211 2 4.793527215124172 2211 3 0.7244858039570801 2211 4 1.712035391003731 2211 5 1.3324628003479937 2211 6 0.17227469913318316 2211 7 -20.88046033979818 2211 8 -13.044181647047477 2211 9 -8.710377437405981 2211 10 -7.075242953761929 2211 11 -9.766908040603088 2211 12 -4.525510656624384 2211 13 -6.920167472091032 2211 14 -4.53500650586426 2211 15 -4.996371237774966 2211 16 -11.668456208154947 2211 17 -7.456924889032834 2211 18 -5.558980257960158 2211 19 -8.409041917133525 2211 20 -6.566315303489603 2211 21 -4.163816849755005 2211 22 -6.008453564598625 2211 23 -7.971960534635869 2211 24 -22.1697375031195 2211 25 -21.956594130538576 2212 1 -1.165210372716647 2212 2 -13.931191885999425 2212 3 -7.81733569247954 2212 4 -19.877852249445706 2212 5 -20.322031581674477 2212 6 -21.087088596746966 2212 7 -9.90695690505824 2212 8 -9.585493170057847 2212 9 -5.1498605908243675 2212 10 -5.367429242000746 2212 11 -5.374989327610621 2212 12 -2.748905664581809 2212 13 -5.792885455867445 2212 14 -3.665497219753547 2212 15 -3.647828751015897 2212 16 -3.9441386536750827 2212 17 -4.021588600610244 2212 18 -3.160952890305154 2212 19 -3.7845780783843637 2212 20 -3.809061735513364 2212 21 -1.733712613024423 2212 22 -3.0409714917725807 2212 23 -3.190863182087103 2212 24 -2.102024058566016 2212 25 -2.7308826486936297 2213 1 -18.93851753660281 2213 2 -13.931191885999425 2213 3 -8.695165094878572 2213 4 -5.611258125397828 2213 5 -20.322031581674477 2213 6 -10.810701219162635 2213 7 -20.88046033979818 2213 8 -10.66095386729991 2213 9 -8.380852591559048 2213 10 -7.181945810229429 2213 11 -6.148080785770649 2213 12 -4.8327276283368406 2213 13 -19.260991442128486 2213 14 -12.360227699720143 2213 15 -6.905599229444789 2213 16 -6.32505147332267 2213 17 -4.925589941830005 2213 18 -4.940519791649685 2213 19 -5.8708133834317735 2213 20 -5.679757899048202 2213 21 -3.7720388220176417 2213 22 -4.544911324824891 2213 23 -4.327409178129653 2213 24 -3.4787690591586724 2213 25 -4.486517960788849 2214 1 -18.93851753660281 2214 2 -13.931191885999425 2214 3 -12.171428227708718 2214 4 -19.877852249445706 2214 5 -20.322031581674477 2214 6 -21.087088596746966 2214 7 -16.453809973050014 2214 8 -14.860860218149003 2214 9 -13.745014764219349 2214 10 -13.697254253817787 2214 11 -14.284215257437792 2214 12 -12.076528027942024 2214 13 -19.260991442128486 2214 14 -16.71168205507078 2214 15 -23.958918054254365 2214 16 -18.25797526898661 2214 17 -23.693816274800625 2214 18 -17.799275854234985 2214 19 -14.739410588323876 2214 20 -13.489588931503487 2214 21 -10.954868835506312 2214 22 -12.047172738980281 2214 23 -11.976836774299613 2214 24 -10.774871977447129 2214 25 -10.522608800465227 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 105494.0 3 3.0 4 667.0 5 2421.0 6 3074.0 7 2972.0 8 2403.0 9 2226.0 10 2114.0 11 1991.0 12 1855.0 13 2110.0 14 3009.0 15 6340.0 16 9431.0 17 9836.0 18 9961.0 19 11364.0 20 13646.0 21 16217.0 22 17130.0 23 19195.0 24 20312.0 25 21038.0 26 17659.0 27 14277.0 28 14854.0 29 17245.0 30 16411.0 31 19166.0 32 25004.0 33 31045.0 34 38163.0 35 43675.0 36 43273.0 37 44671.0 38 33303.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.75878145255463 19.39789877590535 13.031864619972405 25.811455151567614 2 12.636089381221568 20.65299111136489 43.98494451740611 22.725974990007423 3 18.41019003487972 24.989513234542834 29.23253198667487 27.36776474390258 4 11.05159644211347 17.097345759948972 40.17364187249725 31.677415925440304 5 11.540164889866748 38.02422683517037 38.12874447739407 12.306863797568814 6 30.770441094959683 37.89733412004506 18.902715943428277 12.429508841566987 7 26.450346495091814 31.823874508627874 23.307950388241487 18.41782860803882 8 23.04100730982408 39.92589766246285 20.729409323372693 16.303685704340374 9 26.592712893219712 15.061897783875775 20.61496436093922 37.7304249619653 10 14.558849379739842 29.442581989854855 35.73072045602933 20.267848174375974 11 32.91757138425873 21.310792940753924 26.65028651657878 19.121349158408567 12 24.590094520913812 23.881676231670696 33.173518510074196 18.3547107373413 13 28.928182442583466 22.059401849579828 25.34065722968969 23.671758478147012 14 19.191074664349106 23.804614482768375 27.947779431432608 29.05653142144991 15 21.125440259229727 34.67468165258951 23.181548343765865 21.018329744414896 16 19.977322746368294 27.742530361084306 31.435865095820954 20.84428179672645 17 20.067508884003704 29.367478024617515 30.57083501681359 19.994178074565188 18 20.030876655144198 27.00639363494873 33.88610817508917 19.076621534817907 19 23.094285131296008 26.175228767402803 27.461662821176873 23.268823280124312 20 23.1834120342232 28.33481384512288 29.156419966649523 19.325354154004394 21 21.124629864977322 26.373128358632613 26.777131945474835 25.725109830915226 22 22.20430158236106 30.26025799433456 27.886087609921116 19.64935281338326 23 20.53883943711051 29.377312056450734 29.39411287035683 20.689735636081927 24 22.750416393448177 27.284233641201855 29.981683771414826 19.983666193935143 25 21.618173926706294 28.051013439409093 29.811562029896816 20.5192506039878 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 417.0 1 417.0 2 941.5 3 1466.0 4 1466.0 5 1466.0 6 2175.5 7 2885.0 8 2885.0 9 2885.0 10 3398.0 11 3911.0 12 3911.0 13 3911.0 14 5401.0 15 6891.0 16 6891.0 17 6891.0 18 10541.5 19 14192.0 20 14192.0 21 14192.0 22 20783.0 23 27374.0 24 27374.0 25 27374.0 26 37167.5 27 46961.0 28 46961.0 29 46961.0 30 57737.5 31 68514.0 32 68514.0 33 68514.0 34 73763.0 35 79012.0 36 79012.0 37 79012.0 38 80590.0 39 82168.0 40 82168.0 41 82168.0 42 81370.0 43 80572.0 44 80572.0 45 80572.0 46 77863.0 47 75154.0 48 75154.0 49 75154.0 50 67617.5 51 60081.0 52 60081.0 53 60081.0 54 50251.5 55 40422.0 56 40422.0 57 40422.0 58 33110.0 59 25798.0 60 25798.0 61 25798.0 62 20453.5 63 15109.0 64 15109.0 65 15109.0 66 11378.5 67 7648.0 68 7648.0 69 7648.0 70 5398.5 71 3149.0 72 3149.0 73 3149.0 74 2194.0 75 1239.0 76 1239.0 77 1239.0 78 834.0 79 429.0 80 429.0 81 429.0 82 279.5 83 130.0 84 130.0 85 130.0 86 76.0 87 22.0 88 22.0 89 22.0 90 15.5 91 9.0 92 9.0 93 9.0 94 5.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.043104318978177 2 34.68903201746548 3 4.79803590990669 4 12.30275578621874 5 12.284420135031194 6 13.093674977274668 7 8.807017271251096 8 7.148262386276231 9 0.11203393649338442 10 0.9949421572359782 11 4.820877780453885 12 4.585622052505225 13 14.257367280185843 14 1.1271763874105554 15 2.3668528719379074 16 2.4260552711112493 17 2.3147982689902187 18 1.0609815788860315 19 1.0014684059637482 20 0.29290425837729483 21 3.653300805680944 22 1.1525044479492816 23 0.11327703148915011 24 8.292686716753035 25 0.11560783460621081 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 643555.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.83899017174375 #Duplication Level Percentage of deduplicated Percentage of total 1 96.4984655523525 85.72826232793795 2 2.349277526883314 4.174148862429703 3 0.48327289762483483 1.2880042860708847 4 0.19647541487168124 0.6981870980309824 5 0.11198110635848964 0.49741442036014255 6 0.0678758221350255 0.36180116973315346 7 0.05216553181495925 0.3244033217749065 8 0.03399488278460676 0.24160568460730086 9 0.02732628775509293 0.21848758283744482 >10 0.15142397616335734 2.5814399137331705 >50 0.01569564549160317 0.9686089880225882 >100 0.010950323422625616 1.9360653287608938 >500 5.475161709675726E-4 0.36448160609439906 >1k 5.475161709675726E-4 0.6170894096064425 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1503 0.2335464723294823 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1214 0.18863966560744613 No Hit CTGTAGGACGTGGAATATGGCAAGA 1089 0.16921630629860696 No Hit CTTTAGGACGTGAAATATGGCGAGG 935 0.14528672763011707 No Hit GTCCTACAGTGTGCATTTCTCATTT 759 0.11793863772327151 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.5538687447071346E-4 0.0 11 0.0 0.0 0.0 1.5538687447071346E-4 0.0 12 0.0 0.0 0.0 1.5538687447071346E-4 0.0 13 0.0 0.0 0.0 1.5538687447071346E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGGGG 30 5.0552166E-4 20.23012 7 ACGGGGA 35 0.0018774729 16.639595 8 AAGTGTC 55 6.202954E-6 16.551914 7 TGTAGGA 745 0.0 16.005522 2 CTGTAGG 710 0.0 15.641227 1 GGACCTC 40 0.00302208 15.494004 6 TAGGACC 315 0.0 14.882639 4 CCTACAC 75 3.7446007E-6 14.463323 3 GTGCTAA 65 8.943693E-4 14.237526 1 TGTAGAA 200 0.0 14.227635 2 CTAGAGT 45 0.0053806575 14.206155 4 TAGACTC 45 0.006603913 13.773877 5 GGACGGG 60 2.0115048E-4 13.772447 6 TTAGGAC 595 0.0 13.673308 3 GTAGGAC 875 0.0 13.6368475 3 GTCCTAC 1030 0.0 13.605625 1 CAAGACT 55 0.0012932109 13.560421 4 TTTAGGA 560 0.0 13.550131 2 TCCTAGA 70 0.0012997125 13.550131 2 TCGTCAA 70 0.0012997125 13.550131 2 >>END_MODULE