FastQCFastQC Report
Thu 26 May 2016
SRR1926658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926658_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643555
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA31630.4914886839508667No Hit
GTCCTACAGTGGACATTTCTAAATT28870.4486019065969497No Hit
CTTTAGGACGTGAAATATGGCGAGG27280.4238953935561063No Hit
GTCCTAAAGTGTGTATTTCTCATTT25270.39266263178749294No Hit
GTCCTACAGTGTGCATTTCTCATTT17590.273325512193985No Hit
CTGTAGGACCTGGAATATGGCGAGA15170.23572188857207232No Hit
CTGAAGGACCTGGAATATGGCGAGA14960.23245876420818734No Hit
GAATATGGCAAGAAAACTGAAAATC9500.1476175307471778No Hit
GTCCTTCAGTGTGCATTTCTCATTT9080.14109128201940782No Hit
CCCATGTACTCTGCGTTGATACCAC8620.133943485793755No Hit
GGAATATGGCGAGAAAACTGAAAAT8050.12508643394892432No Hit
GTACATGGGAAGCAGTGGTATCAAC7840.12182330958503935No Hit
ACAGTGGACATTTCTAAATTTTCCA7210.11203393649338442No Hit
GATATACACTGTTCTACAAATCCCG7120.11063545462314799No Hit
GCCATATTCCACGTCCTACAGTGGA6910.10737233025926299No Hit
CCATATTTCACGTCCTAAAGTGTGT6770.105196914016673No Hit
CTGTAGGACATGGAATATGGCAAGA6760.1050415271422023No Hit
ACCTGGAATATGGCGAGAAAACTGA6720.10441997964431944No Hit
CCATATTCCAGGTCCTTCAGTGTGC6700.10410920589537802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAGT400.005142724614.3044884
TACTAGG500.001454539413.35192
TAGGACC5150.013.1471674
TCCAATA603.9540936E-412.7160962
GCCGGTT604.154135E-412.64194111
ACTGTTC2650.012.5480448
GACGTGG7450.012.4965427
CAAGACT701.0508481E-412.260994
CGGTTTC701.10902685E-412.19044313
AGACTGT959.898849E-712.0458856
GTGTAGG802.7572329E-511.9176111
GTTTAGG657.774906E-411.7342631
GACTGGA658.012717E-411.6922127
CCGGTTT658.139292E-411.67039112
GTCCTAA7550.011.6176991
CTACACT1406.348273E-1011.5798244
GGACCTG9500.011.5439736
AGGACCT9900.011.4628555
GTGCTAA752.0024354E-411.44090751
CGTGGAA8600.011.3787019
Produced by FastQC (version 0.11.5)