##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926658_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 643555 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.14180450777323 31.0 28.0 31.0 16.0 34.0 2 28.715671543224744 31.0 28.0 33.0 23.0 34.0 3 29.073477791330966 31.0 28.0 33.0 24.0 34.0 4 32.79866988835453 35.0 33.0 37.0 27.0 37.0 5 32.42794322163607 35.0 33.0 37.0 25.0 37.0 6 32.55769747729409 35.0 33.0 37.0 25.0 37.0 7 32.41203782116525 35.0 33.0 37.0 23.0 37.0 8 32.37399445268858 35.0 33.0 37.0 23.0 37.0 9 33.60991989806621 37.0 33.0 39.0 22.0 39.0 10 33.46323313469711 37.0 33.0 39.0 19.0 39.0 11 33.675640776623595 37.0 34.0 39.0 23.0 39.0 12 33.487757845094826 37.0 33.0 39.0 22.0 39.0 13 33.62020961689366 37.0 33.0 39.0 23.0 39.0 14 34.25109586593221 38.0 33.0 40.0 18.0 41.0 15 34.41512380449223 38.0 34.0 40.0 22.0 41.0 16 34.395980141557445 38.0 34.0 40.0 21.0 41.0 17 34.32621143492009 38.0 34.0 40.0 19.0 41.0 18 34.3029593430243 38.0 34.0 40.0 19.0 41.0 19 34.32524337469214 39.0 34.0 40.0 18.0 41.0 20 34.31358003589437 39.0 34.0 40.0 18.0 41.0 21 34.217930091445176 39.0 34.0 40.0 18.0 41.0 22 34.15176169868931 39.0 34.0 40.0 18.0 41.0 23 33.7974454397837 39.0 33.0 40.0 10.0 41.0 24 33.890056016968245 39.0 34.0 40.0 10.0 41.0 25 33.68254772319382 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7091249465095615 1101 2 0.7101716678132846 1101 3 0.6206320531509348 1101 4 0.468076507564561 1101 5 0.5265115019394102 1101 6 0.010658462341901043 1101 7 0.19724754385476473 1101 8 0.2962099466782604 1101 9 0.7541799050552029 1101 10 0.7414095450434672 1101 11 0.5613185373100791 1101 12 0.34703463141725166 1101 13 0.4757952062992672 1101 14 0.5931493582494198 1101 15 0.5024426459834359 1101 16 0.6748978399911252 1101 17 0.7115172930368701 1101 18 0.7728630195468327 1101 19 0.5897124850574613 1101 20 0.6740682815517971 1101 21 0.5530197819948484 1101 22 0.32262640268348264 1101 23 0.6644195439709009 1101 24 0.5092793184029887 1101 25 0.5957255691512557 1102 1 0.8594172499321537 1102 2 1.0068529272695024 1102 3 0.7745815065927744 1102 4 0.6984053377241892 1102 5 0.8402475246432886 1102 6 0.36614955894975054 1102 7 0.7075833570670582 1102 8 0.8580054864771824 1102 9 1.1643894861615678 1102 10 1.2213601265168919 1102 11 0.9934038737947404 1102 12 1.0481555008968328 1102 13 1.1294939860555786 1102 14 1.4961834051074874 1102 15 1.3340928845710707 1102 16 1.331479367131024 1102 17 1.2984070592836545 1102 18 1.3878192859413403 1102 19 1.4196572473203233 1102 20 1.5032431174948258 1102 21 1.1218834244625526 1102 22 1.3598648739321106 1102 23 1.5080439273583934 1102 24 1.386802841442588 1102 25 1.5304385030181678 1103 1 0.7640079841011662 1103 2 0.6348579645755237 1103 3 0.45266922540839616 1103 4 0.1979544866089853 1103 5 0.5324644589168344 1103 6 0.047189970492759414 1103 7 0.63738660258376 1103 8 0.6898792848443378 1103 9 0.9090213012728725 1103 10 0.7074586290798592 1103 11 0.5460739822129739 1103 12 0.4693986884600676 1103 13 0.38683368938026064 1103 14 0.7066880832609286 1103 15 0.7628874462368032 1103 16 0.7311474184455236 1103 17 0.3975897197025944 1103 18 0.6427014686109302 1103 19 0.678108031189943 1103 20 0.9122246830332941 1103 21 0.7712924274244131 1103 22 0.6408383455468254 1103 23 1.0584613085094574 1103 24 0.8517678890188449 1103 25 0.8219496642578079 1104 1 0.9740293407753846 1104 2 1.0597803005783177 1104 3 0.8240259736290447 1104 4 0.537677308650018 1104 5 0.7470057936275722 1104 6 0.6395083758610909 1104 7 0.7278113236371766 1104 8 0.8562696812970287 1104 9 1.0046238688098441 1104 10 1.138628865305698 1104 11 0.8830669092021566 1104 12 0.9746816162928624 1104 13 0.9640751411604995 1104 14 1.5030633687195945 1104 15 1.2871530978077672 1104 16 0.9469800697367674 1104 17 1.1335010488040638 1104 18 1.1798329253788324 1104 19 1.0172802861617 1104 20 0.9519491330952334 1104 21 1.2883422463440866 1104 22 1.1771580118913434 1104 23 1.3092275883484916 1104 24 1.1826216971817374 1104 25 1.551187587672743 1105 1 1.0178619335180805 1105 2 0.9156249357590269 1105 3 1.0527852551355075 1105 4 0.6842556351564042 1105 5 0.7718425983287531 1105 6 0.37404571176115553 1105 7 0.5767221488047198 1105 8 0.5706829742959201 1105 9 1.0381197943551541 1105 10 1.1777183006716712 1105 11 0.8867064176622605 1105 12 0.9691416605835599 1105 13 0.8076558848389439 1105 14 1.1762016396454982 1105 15 1.2619797706500435 1105 16 1.2863937245877537 1105 17 1.200907582405648 1105 18 1.236894107332546 1105 19 1.0754878155802743 1105 20 1.228598093132284 1105 21 1.3105795055315141 1105 22 1.4711867284743008 1105 23 1.694245463842421 1105 24 1.5788454086263997 1105 25 1.7752087777319403 1106 1 1.0560032917562907 1106 2 1.0412129675819237 1106 3 1.074562610145307 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1107 16 0.9567873359504446 1107 17 0.7676842244895781 1107 18 0.8842582912861943 1107 19 1.060299707301212 1107 20 0.9209026890984191 1107 21 1.1801450666936546 1107 22 1.0210587832176188 1107 23 1.138797727323393 1107 24 1.0411978762447447 1107 25 1.2349836515836685 1108 1 0.9476028596231387 1108 2 0.7384011480650372 1108 3 0.5977201361830176 1108 4 0.4331439165487865 1108 5 -0.6886451279052821 1108 6 -0.04621406309129128 1108 7 0.47472441990704084 1108 8 0.6197373826176857 1108 9 1.0927552263552656 1108 10 0.9565472810588815 1108 11 0.7611291791544588 1108 12 0.9676591354542339 1108 13 0.6870932963430576 1108 14 1.2329154106567017 1108 15 0.9020072481843542 1108 16 0.8757833307898295 1108 17 1.084563356192639 1108 18 0.9730158496325245 1108 19 1.0840044493019363 1108 20 1.0271204747055194 1108 21 0.9993172212511823 1108 22 1.218353458227142 1108 23 1.4959936518672592 1108 24 1.1832348045412147 1108 25 1.1836763444280507 1109 1 0.8544842645268709 1109 2 -0.30289674714865455 1109 3 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1110 16 0.746268580307337 1110 17 1.0725010457432376 1110 18 1.3932667456898713 1110 19 1.1955291253427447 1110 20 1.3401891264838426 1110 21 1.3015642573691864 1110 22 1.4686291681802714 1110 23 1.5268042391294614 1110 24 1.2188703159831817 1110 25 1.3941808936422788 1111 1 0.34379952234031563 1111 2 0.43096070250996377 1111 3 0.5968448240017246 1111 4 0.5283047323485022 1111 5 0.7467427434362364 1111 6 0.5495158151315422 1111 7 0.5777513647755157 1111 8 0.6620662824473698 1111 9 1.102990319584407 1111 10 1.123745048319286 1111 11 0.6310128188470543 1111 12 0.6873825689264876 1111 13 0.5438377628588995 1111 14 0.7719149850461875 1111 15 0.6928382983326671 1111 16 0.6902076578981493 1111 17 0.6508582159419305 1111 18 0.7712202551028255 1111 19 0.7174837166678287 1111 20 0.6281343475640497 1111 21 0.8421209359621997 1111 22 0.8604719619178383 1111 23 1.2034904561382547 1111 24 0.9824970309879077 1111 25 1.0466166195199094 1112 1 0.21644029264360398 1112 2 -0.11776366654999038 1112 3 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0.8813466317062932 2202 23 1.0379710321331928 2202 24 0.9004470961660402 2202 25 0.7389266753879724 2203 1 0.2610762300225069 2203 2 0.22269010389099364 2203 3 0.1680681941971116 2203 4 -0.16584406574089883 2203 5 0.23036249318962376 2203 6 0.18972141193336967 2203 7 0.19047483015460642 2203 8 0.25462810859000484 2203 9 0.5273279275313811 2203 10 0.47075887591563514 2203 11 0.24022314022185043 2203 12 0.3992528421902506 2203 13 0.12808399444951135 2203 14 0.4880252596944459 2203 15 0.363952639921024 2203 16 0.45293198858365 2203 17 0.47302909665079085 2203 18 0.3011165913955054 2203 19 0.6605205960268137 2203 20 0.46582882517856916 2203 21 0.5785226458076522 2203 22 0.5944940791495483 2203 23 0.8728309195963675 2203 24 0.6943413450595202 2203 25 0.8958196723209042 2204 1 0.3769945873254237 2204 2 0.39438292408911835 2204 3 0.2166794655155826 2204 4 -0.15051645065690167 2204 5 -5.437370568017741E-4 2204 6 -0.03924233608839245 2204 7 0.2394998209651149 2204 8 0.20915126227064462 2204 9 0.6312845623462593 2204 10 0.6147570979984138 2204 11 0.1873008810365846 2204 12 0.32243789784244115 2204 13 0.049004358830259775 2204 14 0.5694387961655849 2204 15 0.6598359002766969 2204 16 0.45142860269717033 2204 17 0.5743526914271584 2204 18 0.7322261440094024 2204 19 0.6552614369078356 2204 20 0.47688928513006346 2204 21 0.8330762517036945 2204 22 0.7803171886898568 2204 23 1.0295944642957622 2204 24 0.8216277604492745 2204 25 0.9491225841986548 2205 1 0.630444743463876 2205 2 0.734706354784521 2205 3 0.6933900903759778 2205 4 0.5467567106102464 2205 5 0.6762576948328132 2205 6 0.39306116961770954 2205 7 0.5821883221965578 2205 8 0.46203058965626553 2205 9 0.8886976715526629 2205 10 0.8505466008810245 2205 11 0.8015892648904526 2205 12 0.7133628886084509 2205 13 0.6187732096833614 2205 14 1.1616772770311456 2205 15 0.9474511062203206 2205 16 0.9329350650334263 2205 17 0.7081331982822192 2205 18 0.9251472835062202 2205 19 0.9458884579847009 2205 20 0.9202611749528131 2205 21 0.9303463471386735 2205 22 1.0612050828865307 2205 23 1.3522368028541507 2205 24 1.010971365780314 2205 25 1.2632382595065863 2206 1 0.6266988858279596 2206 2 0.6222948075998964 2206 3 0.7802174800526274 2206 4 0.6069805594521043 2206 5 0.705804767705466 2206 6 0.42081805349970125 2206 7 0.5049444252438136 2206 8 0.5277417724991054 2206 9 0.7728289606749925 2206 10 0.8581937667374859 2206 11 0.8212498238535062 2206 12 0.5752676095782334 2206 13 0.655132886518075 2206 14 1.0516598121221392 2206 15 0.8027194483147682 2206 16 0.7356790185787716 2206 17 0.6698071073701257 2206 18 0.8209395155617116 2206 19 0.6588127771899437 2206 20 0.8075759774302824 2206 21 0.8167456387997873 2206 22 0.6648368956695805 2206 23 1.357135287175133 2206 24 0.9995790586500775 2206 25 1.0808187893566767 2207 1 -0.6169538429048025 2207 2 0.3008330806874078 2207 3 0.37743737450888304 2207 4 0.5385702300979176 2207 5 0.5548307251702624 2207 6 0.6612346612637694 2207 7 0.9294337214876975 2207 8 0.7839739104375809 2207 9 1.0549464229032424 2207 10 0.9250957037464431 2207 11 0.8079424513855287 2207 12 0.7926998157222727 2207 13 0.7511446097142311 2207 14 -4.517851570774873 2207 15 -1.4979548832196699 2207 16 -0.3753160685800694 2207 17 0.0727956188127763 2207 18 0.5495986786444291 2207 19 0.7791693698846558 2207 20 0.7097410669646607 2207 21 0.8449343583568591 2207 22 0.8329636181151869 2207 23 1.1075743240775466 2207 24 1.026387635645854 2207 25 0.9252452432064331 2208 1 0.45084012178921284 2208 2 0.8818614693569131 2208 3 0.806467119682015 2208 4 0.7826550371154113 2208 5 0.8100187130184864 2208 6 0.7210024096805938 2208 7 0.7824985541311946 2208 8 0.7458293406379468 2208 9 1.1006262568308784 2208 10 0.9857729906824346 2208 11 0.8907955928407603 2208 12 0.8665419987282306 2208 13 0.8676073988048785 2208 14 1.2423445546761727 2208 15 1.027229117416013 2208 16 0.8140077086865745 2208 17 0.8863331276988689 2208 18 1.1206547791505628 2208 19 0.4925725054513066 2208 20 0.7821884350496475 2208 21 0.8932775367470143 2208 22 0.8892641943454436 2208 23 1.305791288035131 2208 24 1.2023017502363587 2208 25 1.1392145345751814 2209 1 -0.005137947956377786 2209 2 0.011974349057592093 2209 3 0.4395785465898143 2209 4 0.7047338857194063 2209 5 0.5661296671920937 2209 6 0.6453980881982773 2209 7 0.8177201338705018 2209 8 0.9891166592550249 2209 9 0.9612885747273197 2209 10 0.5325304376871998 2209 11 0.6838677309252645 2209 12 0.7767895211975215 2209 13 0.8283445182394473 2209 14 1.004890747148167 2209 15 0.8903992325440839 2209 16 0.7201231307747875 2209 17 1.0516661458760765 2209 18 0.9202205266759123 2209 19 0.783840903170649 2209 20 0.7068990294840702 2209 21 0.8576430352221607 2209 22 0.9893418784091139 2209 23 0.901917029564828 2209 24 0.930321051627466 2209 25 1.0301779610909563 2210 1 0.0634100796080439 2210 2 -0.22310341470869588 2210 3 0.6593503503780127 2210 4 1.0426098076117185 2210 5 1.2392702788572976 2210 6 1.236953618278399 2210 7 1.4975767071696922 2210 8 1.2004752005315993 2210 9 1.5638892982296895 2210 10 1.5141435422511265 2210 11 1.23274235357038 2210 12 1.3831223959730181 2210 13 1.1442972358315089 2210 14 1.5422122598795127 2210 15 1.320894283940774 2210 16 1.258446146556821 2210 17 1.299115417915516 2210 18 1.2950676740640432 2210 19 1.4913749447210805 2210 20 1.3976543124524667 2210 21 1.4998867204986297 2210 22 1.682963147850053 2210 23 1.8048317378074117 2210 24 1.7831367241152662 2210 25 1.6468984446147203 2211 1 -0.9016898978791126 2211 2 0.02926043375940779 2211 3 0.5744001856249383 2211 4 0.7604062096501565 2211 5 0.9946477782944783 2211 6 0.8715446008616468 2211 7 0.78475871326863 2211 8 0.8271611054455406 2211 9 0.8485590757618695 2211 10 0.863251215037188 2211 11 0.5187987175416424 2211 12 0.9199922922092796 2211 13 0.951403107590167 2211 14 0.8582209375570073 2211 15 0.545014748114653 2211 16 0.718168624606804 2211 17 0.8186248426100562 2211 18 0.7969556829054483 2211 19 0.8614809597432895 2211 20 0.939681696255505 2211 21 1.1349177268226924 2211 22 1.098064148648234 2211 23 1.2899732617221886 2211 24 1.127271105319089 2211 25 1.4206699597290253 2212 1 0.7805047704635726 2212 2 0.11001541495930311 2212 3 0.35730863299016846 2212 4 0.1181714050205116 2212 5 0.5358260650042936 2212 6 0.6013864080236928 2212 7 0.5393209079737247 2212 8 0.6922561350883214 2212 9 1.0004989962978996 2212 10 0.47184431321304743 2212 11 0.5010167252087072 2212 12 0.5310709365437063 2212 13 0.5121459964472024 2212 14 0.5299873384861726 2212 15 0.4417805058340889 2212 16 -0.08706563237569753 2212 17 0.36378827301292205 2212 18 0.46262721223764913 2212 19 0.5259377415628776 2212 20 0.5726276335590939 2212 21 0.6708005597388578 2212 22 0.7490704962093702 2212 23 0.7673230906284019 2212 24 0.48000640062586797 2212 25 0.28551965080458075 2213 1 0.02601099015665298 2213 2 -0.2548081067890635 2213 3 -0.7474720252540088 2213 4 -1.6831582819605764 2213 5 -2.2873881425281084 2213 6 -0.7033790867486438 2213 7 -0.5148586040401213 2213 8 -0.5182843999686675 2213 9 -0.9624113651586867 2213 10 -0.84778565536012 2213 11 -1.1596418606800825 2213 12 -1.2037295516773767 2213 13 -1.3524730320013916 2213 14 -8.468088395580704 2213 15 -3.9971486113421726 2213 16 -2.455878642207395 2213 17 -1.838329060223593 2213 18 -5.782410221248632 2213 19 -3.2734452546323745 2213 20 -5.410925338748349 2213 21 -2.514398823898045 2213 22 -1.990098566890964 2213 23 -4.862688196547467 2213 24 -2.570622509707828 2213 25 -2.5779311496024846 2214 1 0.24134417627635685 2214 2 0.18589761405664973 2214 3 -1.5698060175013495 2214 4 -2.293990189716162 2214 5 -0.5501312112937207 2214 6 -0.5626353209353745 2214 7 -0.6878794010688907 2214 8 -0.7174484748773473 2214 9 -5.601650625436815 2214 10 -1.6844237572621061 2214 11 -0.9529831932117219 2214 12 -1.3770416720591 2214 13 -1.6911982861444343 2214 14 -3.204762680109173 2214 15 -2.651355772841729 2214 16 -1.870510257841481 2214 17 -1.9676287401669725 2214 18 -1.5140362312361049 2214 19 -4.570299760566591 2214 20 -2.299254047268029 2214 21 -4.437779451826245 2214 22 -6.434322782113739 2214 23 -6.623582226680146 2214 24 -6.086058656683786 2214 25 -4.026751356535183 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23694.0 3 16.0 4 469.0 5 2802.0 6 3242.0 7 2583.0 8 1980.0 9 1559.0 10 1445.0 11 1298.0 12 1214.0 13 1272.0 14 1202.0 15 1219.0 16 1280.0 17 1363.0 18 1589.0 19 1790.0 20 2342.0 21 3054.0 22 3841.0 23 4837.0 24 5837.0 25 6786.0 26 7769.0 27 8781.0 28 9955.0 29 12119.0 30 14785.0 31 18838.0 32 24121.0 33 30936.0 34 40421.0 35 53251.0 36 72651.0 37 106372.0 38 166842.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.665404845517635 20.38940194654316 9.311400248216913 30.633792959722296 2 12.433649845267166 21.489600803274026 41.72408594161653 24.352663409842283 3 18.248175182481752 25.76894141931633 29.00869197577687 26.974191422425047 4 11.890717313350711 18.442269401687085 37.853826557178806 31.8131867277834 5 12.329716682752757 39.79434671043327 35.51979832549234 12.35613828132164 6 31.209326702896966 38.66700775561393 17.940394706465923 12.18327083502318 7 27.586582630393465 31.641555926720947 22.37099970268718 18.40086174019841 8 23.6213681706664 41.12326736988929 19.615770203120913 15.6395942563234 9 26.37792211027113 15.37431656557857 19.980882517778635 38.266878806371665 10 15.446229590742519 30.14897631236227 33.847741422128564 20.557052674766652 11 33.69554914309777 21.943969207771403 26.12864177617829 18.23183987295253 12 25.017636985769204 24.45294265726442 31.844626701930277 18.684793655036096 13 30.22181476330694 23.025848606568204 25.217425084103144 21.534911546021707 14 19.727551618566046 24.679108921821527 26.86435578443583 28.728983675176593 15 21.435741567760925 35.1882239232249 22.265679629301584 21.110354879712588 16 20.353573887059607 28.35661331099864 30.8967699128108 20.393042889130953 17 20.181560230788477 31.064141232850528 28.730150590568005 20.02414794579299 18 20.301583749782452 27.919494791278186 32.33898336689789 19.43993809204147 19 24.00154767491368 26.80383375858136 26.612549841037 22.582068725467956 20 24.096533441897975 28.670854899937687 27.880083723228534 19.3525279349358 21 21.70633699736153 27.031764234236753 26.60320656852416 24.658692199877557 22 23.303214177498425 30.831086698106613 25.952871160973036 19.912827963421933 23 20.937636934895906 30.29831218514686 28.013033871283525 20.75101700867371 24 24.138531152105813 27.269125105663566 28.789592760180994 19.802750982049623 25 21.758513258385065 29.205584604268473 28.512714531003564 20.52318760634289 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 94.0 1 94.0 2 87.0 3 80.0 4 80.0 5 80.0 6 137.0 7 194.0 8 194.0 9 194.0 10 355.0 11 516.0 12 516.0 13 516.0 14 1023.5 15 1531.0 16 1531.0 17 1531.0 18 3394.5 19 5258.0 20 5258.0 21 5258.0 22 9707.5 23 14157.0 24 14157.0 25 14157.0 26 23055.0 27 31953.0 28 31953.0 29 31953.0 30 45656.0 31 59359.0 32 59359.0 33 59359.0 34 66519.0 35 73679.0 36 73679.0 37 73679.0 38 77601.5 39 81524.0 40 81524.0 41 81524.0 42 83726.5 43 85929.0 44 85929.0 45 85929.0 46 87454.5 47 88980.0 48 88980.0 49 88980.0 50 82169.5 51 75359.0 52 75359.0 53 75359.0 54 63297.5 55 51236.0 56 51236.0 57 51236.0 58 42767.0 59 34298.0 60 34298.0 61 34298.0 62 27416.5 63 20535.0 64 20535.0 65 20535.0 66 15802.5 67 11070.0 68 11070.0 69 11070.0 70 8017.0 71 4964.0 72 4964.0 73 4964.0 74 3422.0 75 1880.0 76 1880.0 77 1880.0 78 1273.5 79 667.0 80 667.0 81 667.0 82 437.0 83 207.0 84 207.0 85 207.0 86 135.5 87 64.0 88 64.0 89 64.0 90 41.0 91 18.0 92 18.0 93 18.0 94 10.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08841513157383596 2 0.02921273240049413 3 0.03154353551755484 4 0.03045582739625984 5 0.022064936174841312 6 0.38396096681713293 7 0.17620871564978907 8 0.18320112500097116 9 0.188173504984034 10 0.015072526823659206 11 0.0024861899915314153 12 0.0032631243638849825 13 0.0 14 0.06588403477558251 15 0.002020029368119275 16 0.0018646424936485614 17 0.0034185112383556962 18 0.0029523506149435556 19 0.0013984818702364211 20 9.323212468242807E-4 21 1.5538687447071346E-4 22 0.0 23 1.5538687447071346E-4 24 4.6616062341214035E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 643555.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.00412052536346 #Duplication Level Percentage of deduplicated Percentage of total 1 92.17547978013386 70.9789175447263 2 5.549191625269069 8.546212414611139 3 0.9825065784528617 2.269711649524399 4 0.41942005224949225 1.2918828901669657 5 0.2099556166430603 0.8083723804479611 6 0.13073585656472075 0.6040319793537833 7 0.09287554751059413 0.5006259898055436 8 0.06281186561076152 0.386941797593121 9 0.042740687473341206 0.29620881445806524 >10 0.2700569402366212 3.8649448392268 >50 0.03272254282460161 1.7510039249525575 >100 0.025812059309634816 3.956176036201993 >500 0.0042681357910257485 2.2288117307250253 >1k 0.001422711930341916 2.516158008206322 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 3163 0.4914886839508667 No Hit GTCCTACAGTGGACATTTCTAAATT 2887 0.4486019065969497 No Hit CTTTAGGACGTGAAATATGGCGAGG 2728 0.4238953935561063 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2527 0.39266263178749294 No Hit GTCCTACAGTGTGCATTTCTCATTT 1759 0.273325512193985 No Hit CTGTAGGACCTGGAATATGGCGAGA 1517 0.23572188857207232 No Hit CTGAAGGACCTGGAATATGGCGAGA 1496 0.23245876420818734 No Hit GAATATGGCAAGAAAACTGAAAATC 950 0.1476175307471778 No Hit GTCCTTCAGTGTGCATTTCTCATTT 908 0.14109128201940782 No Hit CCCATGTACTCTGCGTTGATACCAC 862 0.133943485793755 No Hit GGAATATGGCGAGAAAACTGAAAAT 805 0.12508643394892432 No Hit GTACATGGGAAGCAGTGGTATCAAC 784 0.12182330958503935 No Hit ACAGTGGACATTTCTAAATTTTCCA 721 0.11203393649338442 No Hit GATATACACTGTTCTACAAATCCCG 712 0.11063545462314799 No Hit GCCATATTCCACGTCCTACAGTGGA 691 0.10737233025926299 No Hit CCATATTTCACGTCCTAAAGTGTGT 677 0.105196914016673 No Hit CTGTAGGACATGGAATATGGCAAGA 676 0.1050415271422023 No Hit ACCTGGAATATGGCGAGAAAACTGA 672 0.10441997964431944 No Hit CCATATTCCAGGTCCTTCAGTGTGC 670 0.10410920589537802 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.5538687447071346E-4 0.0 5 1.5538687447071346E-4 0.0 0.0 1.5538687447071346E-4 0.0 6 1.5538687447071346E-4 0.0 0.0 1.5538687447071346E-4 1.5538687447071346E-4 7 1.5538687447071346E-4 0.0 0.0 1.5538687447071346E-4 1.5538687447071346E-4 8 1.5538687447071346E-4 0.0 0.0 1.5538687447071346E-4 1.5538687447071346E-4 9 1.5538687447071346E-4 0.0 0.0 1.5538687447071346E-4 1.5538687447071346E-4 10 1.5538687447071346E-4 0.0 0.0 1.5538687447071346E-4 1.5538687447071346E-4 11 1.5538687447071346E-4 0.0 0.0 1.5538687447071346E-4 1.5538687447071346E-4 12 1.5538687447071346E-4 0.0 0.0 1.5538687447071346E-4 1.5538687447071346E-4 13 1.5538687447071346E-4 0.0 0.0 1.5538687447071346E-4 1.5538687447071346E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGAGT 40 0.0051427246 14.304488 4 TACTAGG 50 0.0014545394 13.3519 2 TAGGACC 515 0.0 13.147167 4 TCCAATA 60 3.9540936E-4 12.716096 2 GCCGGTT 60 4.154135E-4 12.641941 11 ACTGTTC 265 0.0 12.548044 8 GACGTGG 745 0.0 12.496542 7 CAAGACT 70 1.0508481E-4 12.26099 4 CGGTTTC 70 1.10902685E-4 12.190443 13 AGACTGT 95 9.898849E-7 12.045885 6 GTGTAGG 80 2.7572329E-5 11.917611 1 GTTTAGG 65 7.774906E-4 11.734263 1 GACTGGA 65 8.012717E-4 11.692212 7 CCGGTTT 65 8.139292E-4 11.670391 12 GTCCTAA 755 0.0 11.617699 1 CTACACT 140 6.348273E-10 11.579824 4 GGACCTG 950 0.0 11.543973 6 AGGACCT 990 0.0 11.462855 5 GTGCTAA 75 2.0024354E-4 11.4409075 1 CGTGGAA 860 0.0 11.378701 9 >>END_MODULE