FastQCFastQC Report
Thu 26 May 2016
SRR1926657_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926657_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756452
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11390.15057135151999068No Hit
GTCCTAAAGTGTGTATTTCTCATTT9280.12267797560188883No Hit
CTGTAGGACGTGGAATATGGCAAGA8730.11540719040996654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAAA200.00808930427.3437542
GTCCTAT752.0192601E-819.5642071
GTATAGC552.347921E-416.9772031
GATATAC1201.0913936E-915.5624371
TAGGACC2850.015.4330254
TGTAGGA6750.014.9884272
TCTTGCA852.2062144E-514.4761062
CTGTAGG6450.014.2698761
CCTATCC550.001051275413.9380843
GTGTAGA1151.7066304E-713.9191991
GTCCTAA4550.013.7789871
TCCTATA1202.1233427E-713.6718772
TAGACAG851.3957815E-613.4829235
ATAAAGC901.5146616E-613.3849873
GTAGGAC7250.013.2926753
AGACAGG550.001534867213.2564486
TAAGACA901.909888E-613.1117984
TCCTAAA5450.013.0447262
TCCAACG857.119888E-813.03332718
TCTAGGA1051.1086775E-513.0208352
Produced by FastQC (version 0.11.5)