FastQCFastQC Report
Thu 26 May 2016
SRR1926657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926657_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756452
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT23590.311850586686267No Hit
CTGTAGGACGTGGAATATGGCAAGA23520.3109252140254768No Hit
CTTTAGGACGTGAAATATGGCGAGG21610.28567575999534667No Hit
GTCCTAAAGTGTGTATTTCTCATTT20340.2688868560067261No Hit
GTATCAACGCAGAGTACTTTTTTTT14350.18970139546197248No Hit
GTCCTACAGTGTGCATTTCTCATTT14210.18785065014039226No Hit
CTGTAGGACCTGGAATATGGCGAGA12010.1587675093727031No Hit
CTGAAGGACCTGGAATATGGCGAGA11260.14885280229280906No Hit
GTACATGGGAAGCAGTGGTATCAAC8830.1167291513539524No Hit
GGTATCAACGCAGAGTACTTTTTTT8790.11620036697635805No Hit
CCCATGTACTCTGCGTTGATACCAC8440.11157350367240751No Hit
TATCAACGCAGAGTACTTTTTTTTT7890.10430271848048522No Hit
GAATATGGCAAGAAAACTGAAAATC7720.10205538487570924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG602.4623427E-514.3117225
TAGGACC3800.013.8095564
TAAGGGT706.9340113E-613.6302124
GGCGAGG5500.013.26048719
TCGTCAA701.0450685E-412.2688242
GTATTAA701.04962724E-412.2631071
GTCTAGG802.747261E-511.9224651
TGGCGAG13750.011.64167318
TGTAGGA12850.011.5845712
TCTAGAA751.9871356E-411.4509022
AGGACCT9100.011.4284085
ACGAAAT1251.879198E-811.37741212
CGAAATC1251.881017E-811.37665813
GTAGGAC12000.011.2903583
TCTGTAC1104.685262E-711.2759023
TCTAGGA1701.0913936E-1111.2263752
GTCTAGA855.102523E-511.2211441
AGAACGT600.00568498611.1313395
GGACCTG9000.011.1305986
GTATAAC600.00569909511.1276351
Produced by FastQC (version 0.11.5)