FastQCFastQC Report
Thu 26 May 2016
SRR1926656_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926656_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679131
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9940.14636351455021196No Hit
GTCCTAAAGTGTGTATTTCTCATTT7980.11750310323045186No Hit
CTGTAGGACGTGGAATATGGCAAGA6850.10086419262263098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2700.016.6688374
GTCCTAA3800.015.8189071
GACCTAC604.5058434E-415.5845521
CTAAGAC753.4160385E-614.5921463
TGTAGGA6700.014.3063342
TAATTCA450.005180796614.2875744
CTGTAGG6200.014.0045531
CAGATGT851.0162403E-613.8673524
TCCTAAA4250.013.8543022
CTTTAGG3600.013.7292491
TTTAGGA3700.013.6932072
TCTAGGA700.00120077213.6932072
GTCCAAG600.00808431313.3581871
GTCCTAC6450.013.2546361
ACTATAC500.00904949713.132933
GTAGGAC6900.013.00604253
AGGACCT4900.012.9813575
GGACCTG4400.012.7885226
ACCTACA750.00201915812.7803262
TCCTACA7100.012.7288952
Produced by FastQC (version 0.11.5)