FastQCFastQC Report
Thu 26 May 2016
SRR1926656_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926656_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679131
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA18330.26990374463836875No Hit
GTCCTACAGTGGACATTTCTAAATT18180.2676950396904279No Hit
CTTTAGGACGTGAAATATGGCGAGG17820.2623941478153699No Hit
GTCCTAAAGTGTGTATTTCTCATTT16670.2454607432144903No Hit
GTCCTACAGTGTGCATTTCTCATTT13390.19716372835285093No Hit
CTGTAGGACCTGGAATATGGCGAGA11950.175960160852619No Hit
GTATCAACGCAGAGTACTTTTTTTT10470.15416760536626956No Hit
CCCATGTACTCTGCGTTGATACCAC10320.1519589004183287No Hit
CTGAAGGACCTGGAATATGGCGAGA9900.1457745265640944No Hit
GTACATGGGAAGCAGTGGTATCAAC8730.12854662797015598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAG400.005134884314.30794051
TAGGACC6150.013.4986724
CGAAATC655.5584504E-513.124619513
CTAGACA751.40995835E-512.7228854
GGCGAGG4700.012.697329519
GTAGGAC12150.012.5658133
CTGTAGG12300.012.407971
TGTAGGA12550.012.3961672
ATAAGAC701.0450229E-412.2684973
GTCCTAC10450.012.140071
TATACTT1302.4265319E-911.7442035
GGACCTG9600.011.728916
AGGACCT9800.011.6842835
TGGCGAG13250.011.58160818
GTCCTAA4900.011.4852861
CTATACT1001.8242317E-611.4505974
GCCATAT2600.011.3729791
GGCGAGA8550.011.18986919
TCAGTAC600.00567945811.13252453
ACTATAC951.2919176E-511.0488213
Produced by FastQC (version 0.11.5)