FastQCFastQC Report
Thu 26 May 2016
SRR1926655_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926655_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754387
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9620.1275207552622195No Hit
GTCCTAAAGTGTGTATTTCTCATTT8520.11293937992038569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCAGG551.0921773E-519.3557451
CGGGAAA250.00735229218.3574399
CTGAACT608.366662E-616.0393124
GTGTAAC604.640702E-415.52491951
CTCCTAG801.6067037E-514.9704581
GTCAATA550.003964223414.8744412
GGATTAT751.556547E-414.1942121
TAGGACC2900.013.6426354
TTAGTAC806.8613153E-613.6423383
TATAGGA802.1891591E-413.6349042
TAGGAGT550.001259143213.6091154
GTCTAGG700.001490513613.3070751
GGTATAA700.001490513613.3070751
GTTCAGT700.001490513613.3070751
TGTAGGA6950.013.144442
TTAGGAC4400.012.6501673
GTCCTAA4050.012.4856511
ATAATAC704.6506146E-412.4729953
GGATAAA750.002504036312.4199351
GGACGTG6850.012.292466
Produced by FastQC (version 0.11.5)