FastQCFastQC Report
Thu 26 May 2016
SRR1926655_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926655_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754387
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGGACGTGAAATATGGCGAGG19740.2616694084070908No Hit
CTGTAGGACGTGGAATATGGCAAGA19590.2596810390422953No Hit
GTCCTAAAGTGTGTATTTCTCATTT19090.2530531411596435No Hit
GTCCTACAGTGGACATTTCTAAATT18530.2456298955310736No Hit
GTCCTACAGTGTGCATTTCTCATTT15240.2020183274632251No Hit
CTGTAGGACCTGGAATATGGCGAGA12090.16026257080251913No Hit
CTGAAGGACCTGGAATATGGCGAGA9960.1320277258224227No Hit
GTATCAACGCAGAGTACTTTTTTTT9140.12115797329487385No Hit
CCCATGTACTCTGCGTTGATACCAC8630.11439751745456908No Hit
GTACATGGGAAGCAGTGGTATCAAC8500.11267426400507963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC456.8832946E-414.74161313
CTATACT602.4557437E-514.3157824
TAGGACC4950.013.8819694
TTATACT655.2148258E-513.2145674
CTAAGAC801.8877854E-613.1271853
ACGAAAT604.1730425E-412.63566912
GGACCTG7750.012.43779856
AGGACCT8750.012.4342795
TCCTATA1001.3477074E-712.4119852
CCCTTAT701.0462004E-412.2673911
TAGCTCC550.002961930112.1467244
CCGGTTT550.00312270112.06131914
CGGTTTC550.003136689512.05412315
TAGAAAT2800.011.9298174
TCTAGGA1302.406523E-911.7509922
GTCCTAT1156.6389475E-811.6155011
TGGCGAG13800.011.46064118
CTACACC1001.8212322E-611.4526254
CCCTTAG751.9892765E-411.4495661
GTAGGAC13250.011.31234553
Produced by FastQC (version 0.11.5)