FastQCFastQC Report
Thu 26 May 2016
SRR1926654_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926654_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639527
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8270.12931432136563428No Hit
GTCCTAAAGTGTGTATTTCTCATTT7630.11930692527446067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG1053.1322998E-916.5140931
TGTAGGA5650.015.9696772
GAACTGT400.002848995815.6315676
GTATAAT801.5701062E-515.0055951
TAGGACC2000.014.9970514
TGTAGAA2650.014.960732
GTAGAAC1800.014.5889913
GTGTTGG658.3622977E-414.3643291
TCCTGAG600.006935166713.6710122
CTGTAGG5600.013.5764891
AGAACAG1550.013.4493985
GTGTAGA1409.580617E-913.3383051
TATACAC2100.013.0258853
GTCCTAA2900.012.8783651
CTTTAGG2700.012.8442951
CCAACGA607.087347E-512.78796519
GTAGGAC6000.012.76536753
TCTACTC600.00203799412.76536753
GAACAGT1151.9370418E-812.6864886
GTGTTAA957.9095604E-512.636291
Produced by FastQC (version 0.11.5)