FastQCFastQC Report
Thu 26 May 2016
SRR1926654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926654_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639527
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17150.26816694213066844No Hit
CTGTAGGACGTGGAATATGGCAAGA16800.2626941473933079No Hit
CTTTAGGACGTGAAATATGGCGAGG15640.24455574197805569No Hit
GTCCTAAAGTGTGTATTTCTCATTT15450.2415847962634885No Hit
GTCCTACAGTGTGCATTTCTCATTT13260.20734073776400372No Hit
GTATCAACGCAGAGTACTTTTTTTT11990.18748231114558103No Hit
CTGTAGGACCTGGAATATGGCGAGA10940.17106392693349928No Hit
GTACATGGGAAGCAGTGGTATCAAC10550.16496566994044035No Hit
CCCATGTACTCTGCGTTGATACCAC10150.15871104738345684No Hit
CTGAAGGACCTGGAATATGGCGAGA8250.1290015902377851No Hit
GGTATCAACGCAGAGTACTTTTTTT7880.12321606437257536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT456.5582164E-414.835381
GTACCGT400.005137476614.306676
GGACCTT500.001453706913.3528936
TATAAGT500.001453706913.3528934
GGTCTAC500.001454569713.3518411
TAGGACC3900.013.2061584
GTCCTAT1155.000402E-912.4396041
GGCGAGG3900.012.38959219
TCCAACG1155.515176E-912.35790518
GTCCTAG959.889718E-712.0467741
GTCCTAA4300.011.7549441
TAGAACT907.1194117E-611.6572874
CGTTTCC907.621591E-611.58615614
TTAGAAC751.9922909E-411.4471393
TTGGACA751.9952079E-411.4453364
TCCTATA1354.4019544E-911.3067052
AGACTGC855.10069E-511.2209186
CCGTTTC855.4289354E-511.1524513
TAGTACT600.00569830811.127414
TGGCGAG8650.011.06258818
Produced by FastQC (version 0.11.5)