FastQCFastQC Report
Thu 26 May 2016
SRR1926653_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926653_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660168
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7140.10815428799941833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGA905.977199E-918.2757422
AACGCAC350.001350341717.4791167
GTCCTAA2250.017.2199251
GTCCTAT808.365914E-716.7003571
GTTCTAG604.4999798E-415.5870021
TAGGACC2150.015.4721964
GTGTAGA708.357057E-515.2688991
TGGCGGG406.4185634E-414.877233518
GTCTAGA550.00453689814.5748581
AACTACC350.005736439514.07062416
TCCTAAA2750.013.9560222
TGTAGGA5800.013.94313052
GGGTAGT450.00618710413.9101046
GGACCTG3900.013.9101046
CTGTAGG5300.013.8644491
AATTAGG600.006816624713.7068072
ACTGTTC1500.013.6663518
GTATTAA700.001445709713.3602871
GTGTAGG802.599639E-413.3602861
AGGACCT4150.013.0762435
Produced by FastQC (version 0.11.5)